Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   INQ42_RS03225 Genome accession   NZ_CP063657
Coordinates   722217..723275 (+) Length   352 a.a.
NCBI ID   WP_194035111.1    Uniprot ID   -
Organism   Novilysobacter avium strain H23M41     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 717217..728275
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INQ42_RS03210 (INQ42_03210) - 717375..718667 (+) 1293 WP_194035724.1 citrate synthase -
  INQ42_RS03215 (INQ42_03215) - 718720..719397 (-) 678 WP_194035725.1 hypothetical protein -
  INQ42_RS03220 (INQ42_03220) - 719467..721971 (-) 2505 WP_194035110.1 penicillin-binding protein 1A -
  INQ42_RS03225 (INQ42_03225) pilM 722217..723275 (+) 1059 WP_194035111.1 pilus assembly protein PilM Machinery gene
  INQ42_RS03230 (INQ42_03230) - 723275..724063 (+) 789 WP_194035112.1 PilN domain-containing protein -
  INQ42_RS03235 (INQ42_03235) - 724060..724725 (+) 666 WP_194035113.1 type 4a pilus biogenesis protein PilO -
  INQ42_RS03240 (INQ42_03240) - 724725..725279 (+) 555 WP_194035114.1 pilus assembly protein PilP -
  INQ42_RS03245 (INQ42_03245) - 725299..727245 (+) 1947 WP_407070781.1 type IV pilus secretin PilQ -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37623.23 Da        Isoelectric Point: 4.5746

>NTDB_id=496141 INQ42_RS03225 WP_194035111.1 722217..723275(+) (pilM) [Novilysobacter avium strain H23M41]
MGVIKKSQPALVGVDISSTAVKLLQLSRAGNRYRVEHYAVEPLPPNAVVEMNIVEPEAVGEAIKRAMARSGTRAKHAAAA
VSGSAVITKVIPMPAELDGDDLEAQVELEAVNYVPYPIEEVNLDFEVLGPMPGSPESVQVLLAASRSENVEVRASALELG
GLIPQVMDVEAFAIENAFALIADQLPIPRDGLVALIDAGATMTTLNVIRHGRSIYSREQVFGGKQLTDEIMRRYGLSYEE
AGLAKRQGGLPETYETETLDPFKEALVQQVSRLLQFFYAGSEHNRVDQIVLAGGCAAIAGIAPLVEEQLGVPTMIANPLA
HMTLGPRVQAHALAQDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=496141 INQ42_RS03225 WP_194035111.1 722217..723275(+) (pilM) [Novilysobacter avium strain H23M41]
TTGGGCGTCATCAAGAAGAGCCAGCCGGCACTCGTCGGCGTGGATATCAGTTCGACTGCGGTCAAGCTTCTGCAGCTGTC
GCGGGCTGGCAACCGGTATCGCGTGGAGCATTACGCGGTGGAGCCGTTGCCACCCAACGCGGTGGTGGAAATGAACATCG
TGGAACCCGAGGCGGTTGGCGAGGCGATCAAGCGCGCCATGGCCCGCTCGGGCACCCGCGCCAAGCACGCTGCAGCAGCC
GTGTCCGGATCGGCGGTGATCACCAAGGTGATCCCGATGCCGGCCGAGTTGGACGGCGATGACCTGGAAGCCCAGGTCGA
GCTGGAAGCGGTCAACTACGTTCCCTACCCGATCGAGGAAGTGAACCTGGACTTCGAAGTGCTCGGGCCGATGCCCGGCA
GCCCGGAGTCGGTGCAGGTGCTTCTGGCCGCATCGCGCTCGGAGAACGTCGAGGTCCGTGCCTCCGCGCTCGAGCTTGGG
GGGCTTATCCCCCAGGTCATGGACGTCGAAGCGTTCGCGATCGAGAACGCGTTCGCCCTCATCGCCGATCAGCTGCCCAT
CCCGCGCGACGGTCTGGTCGCGCTGATCGATGCGGGAGCCACGATGACCACGCTCAACGTCATCCGCCATGGCCGCAGCA
TCTACAGCCGCGAACAGGTATTCGGCGGCAAGCAGCTGACCGACGAGATCATGCGCCGTTACGGCCTGAGCTATGAGGAA
GCCGGTCTGGCGAAGCGCCAGGGCGGACTGCCGGAAACCTACGAGACGGAAACCCTGGATCCGTTCAAGGAAGCCCTCGT
ACAGCAGGTCAGCCGCCTGCTGCAGTTCTTCTACGCGGGCAGCGAACACAACCGGGTCGACCAGATCGTGCTGGCCGGGG
GCTGCGCGGCCATCGCGGGCATCGCGCCGCTGGTCGAGGAGCAGCTGGGCGTGCCGACGATGATCGCCAACCCGCTGGCA
CACATGACGCTGGGACCACGGGTGCAGGCGCATGCACTCGCGCAGGATGCTCCGGCCCTGATGATCGCGTGCGGGCTGGC
ATTGAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

52.011

98.864

0.514
  comM Acinetobacter nosocomialis M2

51.724

98.864

0.511
  comM Acinetobacter baylyi ADP1

50.287

98.864

0.497
  pilM Legionella pneumophila strain ERS1305867

44.857

99.432

0.446