Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   INQ41_RS03145 Genome accession   NZ_CP063656
Coordinates   696888..697946 (+) Length   352 a.a.
NCBI ID   WP_193986133.1    Uniprot ID   A0A7S6ZSN9
Organism   Novilysobacter ciconiae strain H21R20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 691888..702946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INQ41_RS03130 (INQ41_03130) - 692092..693384 (+) 1293 WP_193987181.1 citrate synthase -
  INQ41_RS03135 (INQ41_03135) - 693449..694123 (-) 675 WP_407074246.1 hypothetical protein -
  INQ41_RS03140 (INQ41_03140) - 694154..696643 (-) 2490 WP_193986131.1 penicillin-binding protein 1A -
  INQ41_RS03145 (INQ41_03145) pilM 696888..697946 (+) 1059 WP_193986133.1 pilus assembly protein PilM Machinery gene
  INQ41_RS03150 (INQ41_03150) - 697946..698707 (+) 762 WP_193986135.1 PilN domain-containing protein -
  INQ41_RS03155 (INQ41_03155) - 698704..699369 (+) 666 WP_193986136.1 type 4a pilus biogenesis protein PilO -
  INQ41_RS03160 (INQ41_03160) - 699369..699923 (+) 555 WP_193987184.1 pilus assembly protein PilP -
  INQ41_RS03165 (INQ41_03165) - 699943..701895 (+) 1953 WP_228076685.1 type IV pilus secretin PilQ -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37646.26 Da        Isoelectric Point: 4.5214

>NTDB_id=496121 INQ41_RS03145 WP_193986133.1 696888..697946(+) (pilM) [Novilysobacter ciconiae strain H21R20]
MGVIKKSQSALVGVDISSTAVKLLQLSRAGNRYRVEHYAVEPLPPNAVVEMNIVEPEAVGEAIKRAMARSGTRAKHAAAA
VSGSAVITKVIPMPAELDGDDLEAQVELEAVNYVPYPIEEVNLDFEVLGPMPGSPESVQVLLAASRSENVEVRASVLELG
GLIPQVMDVEAFAIENAFALIVDQLPIPRDGLVALIDAGATMTTLNVIRQGRSIYSREQVFGGKQLTDEIMRRYGLSYEE
AGLAKRQGGLPETYETETLDPFKEALVQQVSRLLQFFYAGSEHNRVDQIVLAGGCAAIAGIAPLVEEQLGVPTMVANPLA
HMTLGPRVQAHALAQDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=496121 INQ41_RS03145 WP_193986133.1 696888..697946(+) (pilM) [Novilysobacter ciconiae strain H21R20]
TTGGGCGTCATCAAGAAGAGCCAGTCGGCACTCGTCGGCGTGGATATCAGTTCGACTGCGGTCAAGCTTCTGCAGCTGTC
GCGGGCAGGCAACCGGTATCGCGTGGAGCACTACGCGGTGGAACCGTTGCCGCCCAACGCGGTGGTGGAGATGAACATCG
TGGAACCCGAGGCGGTTGGCGAGGCGATCAAGCGCGCCATGGCGCGCTCGGGCACCCGCGCCAAACACGCCGCAGCAGCC
GTCTCCGGATCGGCGGTGATCACCAAGGTGATCCCGATGCCGGCCGAGCTGGACGGCGATGATCTGGAAGCCCAGGTCGA
GCTGGAAGCGGTCAACTACGTGCCGTACCCGATCGAGGAAGTGAACCTCGACTTCGAAGTGCTCGGGCCGATGCCCGGCA
GCCCGGAGTCGGTGCAGGTGCTGCTGGCCGCCTCGCGCTCGGAGAACGTCGAGGTCCGTGCCTCCGTGCTCGAGCTTGGG
GGGCTCATCCCCCAGGTCATGGACGTGGAAGCGTTCGCGATCGAGAACGCGTTCGCCCTGATTGTCGATCAGCTGCCCAT
CCCGCGCGACGGGCTGGTGGCGCTGATCGACGCCGGCGCGACGATGACCACGCTCAACGTCATCCGCCAGGGCCGCAGCA
TCTACAGCCGCGAGCAGGTCTTCGGCGGCAAACAGCTGACCGACGAGATCATGCGCCGTTATGGCCTGAGCTATGAGGAA
GCCGGTCTGGCGAAGCGCCAGGGCGGACTGCCGGAGACCTACGAGACGGAGACCCTGGATCCGTTCAAGGAGGCCCTGGT
GCAGCAGGTCAGCCGCCTGTTGCAGTTCTTCTATGCCGGCAGCGAGCACAACCGCGTCGACCAGATCGTGTTGGCCGGCG
GTTGCGCGGCCATCGCCGGCATCGCGCCACTGGTCGAGGAGCAACTGGGCGTGCCGACGATGGTCGCCAACCCGCTCGCC
CACATGACGCTGGGGCCGCGGGTGCAGGCGCACGCGCTGGCGCAGGACGCTCCGGCGTTGATGATCGCGTGCGGCCTGGC
ATTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7S6ZSN9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.724

98.864

0.511
  comM Acinetobacter nosocomialis M2

51.437

98.864

0.509
  comM Acinetobacter baylyi ADP1

50

98.864

0.494
  pilM Legionella pneumophila strain ERS1305867

44.571

99.432

0.443