Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   IPV45_RS28390 Genome accession   NZ_CP063389
Coordinates   6100229..6101293 (-) Length   354 a.a.
NCBI ID   WP_003110888.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain ST1076_d97burn2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 6055284..6107604 6100229..6101293 within 0


Gene organization within MGE regions


Location: 6055284..6107604
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IPV45_RS28220 (IPV45_28205) phnC 6055284..6056111 (-) 828 WP_003118432.1 phosphonate ABC transporter ATP-binding protein -
  IPV45_RS28225 (IPV45_28210) - 6056573..6056809 (+) 237 Protein_5579 IS5/IS1182 family transposase -
  IPV45_RS28230 (IPV45_28215) - 6056865..6058015 (+) 1151 WP_085990595.1 IS3 family transposase -
  IPV45_RS28235 (IPV45_28220) - 6058035..6058787 (+) 753 Protein_5581 IS5-like element ISPre1 family transposase -
  IPV45_RS28240 (IPV45_28225) - 6058971..6059441 (-) 471 WP_225775528.1 hypothetical protein -
  IPV45_RS28245 (IPV45_28230) - 6059434..6061305 (-) 1872 WP_015275425.1 tyrosine-type recombinase/integrase -
  IPV45_RS28250 (IPV45_28235) - 6061298..6063358 (-) 2061 WP_201020692.1 hypothetical protein -
  IPV45_RS28255 (IPV45_28240) - 6063351..6064472 (-) 1122 WP_071562096.1 tyrosine-type recombinase/integrase -
  IPV45_RS28260 (IPV45_28245) - 6064743..6065006 (+) 264 Protein_5586 peptide-methionine (S)-S-oxide reductase -
  IPV45_RS28265 (IPV45_28250) - 6065010..6065846 (-) 837 WP_003095790.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
  IPV45_RS28270 (IPV45_28255) - 6066148..6067950 (+) 1803 WP_003095792.1 acyl-CoA dehydrogenase -
  IPV45_RS28275 (IPV45_28260) - 6068243..6069985 (+) 1743 WP_003095795.1 potassium/proton antiporter -
  IPV45_RS28280 (IPV45_28265) mscK 6070077..6073433 (+) 3357 WP_003095799.1 mechanosensitive channel MscK -
  IPV45_RS28285 (IPV45_28270) selO 6073500..6074960 (+) 1461 WP_003116144.1 protein adenylyltransferase SelO -
  IPV45_RS28290 (IPV45_28275) - 6075111..6076085 (+) 975 WP_003095804.1 sulfite exporter TauE/SafE family protein -
  IPV45_RS28295 (IPV45_28280) - 6076222..6077499 (+) 1278 WP_031686158.1 bifunctional O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase -
  IPV45_RS28300 (IPV45_28285) - 6077590..6078042 (+) 453 WP_003114565.1 YiiD C-terminal domain-containing protein -
  IPV45_RS28305 (IPV45_28290) - 6078155..6078970 (+) 816 WP_003095811.1 universal stress protein -
  IPV45_RS28310 (IPV45_28295) - 6078992..6079759 (-) 768 WP_003095812.1 ParA family protein -
  IPV45_RS28315 (IPV45_28300) - 6079850..6080755 (-) 906 WP_003095817.1 LysR family transcriptional regulator -
  IPV45_RS28320 (IPV45_28305) - 6080931..6082247 (+) 1317 WP_003103690.1 MFS transporter -
  IPV45_RS28325 (IPV45_28310) - 6082275..6083204 (-) 930 WP_031686159.1 oxidoreductase -
  IPV45_RS28330 (IPV45_28315) - 6083316..6084389 (+) 1074 WP_003095821.1 AraC family transcriptional regulator -
  IPV45_RS28335 (IPV45_28320) - 6084463..6085443 (+) 981 WP_031636802.1 hypothetical protein -
  IPV45_RS28340 (IPV45_28325) hemE 6085564..6086631 (-) 1068 WP_003114569.1 uroporphyrinogen decarboxylase -
  IPV45_RS28345 (IPV45_28330) - 6086808..6088241 (-) 1434 WP_031636804.1 FAD-dependent oxidoreductase -
  IPV45_RS28350 (IPV45_28335) gltB 6088270..6092715 (-) 4446 WP_031686160.1 glutamate synthase large subunit -
  IPV45_RS28355 (IPV45_28340) - 6092940..6094601 (-) 1662 WP_031686162.1 AAA family ATPase -
  IPV45_RS28360 (IPV45_28345) aroB 6094608..6095714 (-) 1107 WP_003112148.1 3-dehydroquinate synthase -
  IPV45_RS28365 (IPV45_28350) aroK 6095764..6096282 (-) 519 WP_003095833.1 shikimate kinase AroK -
  IPV45_RS28370 (IPV45_28355) pilQ 6096294..6098438 (-) 2145 WP_031686163.1 type 4a pilus secretin PilQ Machinery gene
  IPV45_RS28375 (IPV45_28360) pilP 6098492..6099016 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  IPV45_RS28380 (IPV45_28365) pilO 6099013..6099636 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  IPV45_RS28385 (IPV45_28370) pilN 6099633..6100229 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  IPV45_RS28390 (IPV45_28375) pilM 6100229..6101293 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  IPV45_RS28395 (IPV45_28380) - 6101478..6103949 (+) 2472 WP_031686164.1 penicillin-binding protein 1A -
  IPV45_RS28400 (IPV45_28385) - 6104045..6105313 (-) 1269 WP_003095842.1 malic enzyme -
  IPV45_RS28405 (IPV45_28390) - 6105416..6106855 (-) 1440 WP_003095844.1 M48 family metalloprotease -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37983.48 Da        Isoelectric Point: 4.5405

>NTDB_id=495173 IPV45_RS28390 WP_003110888.1 6100229..6101293(-) (pilM) [Pseudomonas aeruginosa strain ST1076_d97burn2]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=495173 IPV45_RS28390 WP_003110888.1 6100229..6101293(-) (pilM) [Pseudomonas aeruginosa strain ST1076_d97burn2]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTTGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTTTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.899

100

0.562
  comM Acinetobacter nosocomialis M2

55.899

100

0.562
  comM Acinetobacter baylyi ADP1

51.977

100

0.52
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466