Detailed information NTG0494657

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IM687_RS19795
• Genome accession: NZ_CP063358
• Coordinates: 4204972..4206117 (-)
• Length: 381 a.a.
• NCBI ID: WP_019405534.1
• Uniprot ID: -
• Organism: Stutzerimonas stutzeri strain ZDHY95
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4199972..4211117

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IM687_RS19770 (IM687_19770)	-	4200049..4201065 (+)	1017	WP_011914964.1	aspartate carbamoyltransferase catalytic subunit	-
IM687_RS19775 (IM687_19775)	-	4201065..4202339 (+)	1275	WP_125849504.1	dihydroorotase	-
IM687_RS19780 (IM687_19780)	-	4202397..4202792 (-)	396	WP_048330154.1	NINE protein	-
IM687_RS19785 (IM687_19785)	-	4202928..4204142 (+)	1215	WP_014598001.1	putative DNA modification/repair radical SAM protein	-
IM687_RS19790 (IM687_19790)	-	4204142..4204975 (+)	834	WP_048330155.1	TIGR03915 family putative DNA repair protein	-
IM687_RS19795 (IM687_19795)	pilU	4204972..4206117 (-)	1146	WP_019405534.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IM687_RS19800 (IM687_19800)	pilT	4206177..4207211 (-)	1035	WP_011914970.1	type IV pilus twitching motility protein PilT	Machinery gene
IM687_RS19805 (IM687_19805)	-	4207278..4207973 (+)	696	WP_196111674.1	YggS family pyridoxal phosphate-dependent enzyme	-
IM687_RS19810 (IM687_19810)	proC	4207984..4208808 (+)	825	WP_125849498.1	pyrroline-5-carboxylate reductase	-
IM687_RS19815 (IM687_19815)	-	4208818..4209408 (+)	591	WP_014598006.1	YggT family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42566.88 Da
• Isoelectric Point: 6.8858

>NTDB_id=494657 IM687_RS19795 WP_019405534.1 4204972..4206117(-) (pilU) [Stutzerimonas stutzeri strain ZDHY95]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTPMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=494657 IM687_RS19795 WP_019405534.1 4204972..4206117(-) (pilU) [Stutzerimonas stutzeri strain ZDHY95]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTCGAAAAAGGCGGCTCCGATCTGTTCATCACGGCTGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGAGCAAGACGCCCATGTCGCCGGAGATGACCCGGGAAACCGTGCACGGCG
TGATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAACCACGAATGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGTGTCAGCGCGTTCTATCAGCGAAACCTGGCCGGCATGGTGCTGCGTCGCATCGAGACCAACATCCCGACCATCGA
AGAACTCAAGCTGCCGGACGTCCTGAAGAAGCTGTCGATGACCAAACGTGGTCTGGTGCTGTTCGTCGGCGCCACCGGTA
CCGGCAAGTCGACGTCGCTGGCCTCGATGATCGGCTACCGCAACAAGAACTCCAGCGGGCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCACCAGAATTGCATCGTCACCCAGCGCGAAGTGGGCATCGACACCAGCTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGATCCGTACCCGCGAAACCATGGATTACG
CGGTCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGCCGGACCGGCACAACCAGGTCTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTTCCGACCCCGGACGGCAAGGGTCGTCGTGCGGTCATCGAGGTGCTGATCAATACGCCGCTGGCGGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAATCGCTGATGAAGCGCTCCACCGAGCTGGGTATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTACGTGCAGGGCGAGATCACCTACGAAGATGCCCTGCTGCATGCTGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGGGTTCGGAAACCGACGGTGAGCACCTGACCAGCGTATCCCAGGGCCTGAGCCTGGAGGTCAGCGACG
AGGACCCGGGCCGGCGCTTCCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	95.013	100	0.95
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.429	91.864	0.546
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.953	88.976	0.391