Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   KIM20_RS25535 Genome accession   NZ_CP074868
Coordinates   5019443..5020681 (-) Length   412 a.a.
NCBI ID   WP_000815968.1    Uniprot ID   -
Organism   Escherichia coli strain 2013C_3277     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5014443..5025681
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM20_RS25515 (KIM20_25220) dam 5015055..5015891 (-) 837 WP_000742143.1 adenine-specific DNA-methyltransferase -
  KIM20_RS25520 (KIM20_25225) damX 5015998..5017284 (-) 1287 WP_000343193.1 cell division protein DamX -
  KIM20_RS25525 (KIM20_25230) aroB 5017376..5018464 (-) 1089 WP_000439846.1 3-dehydroquinate synthase -
  KIM20_RS25530 (KIM20_25235) aroK 5018521..5019042 (-) 522 WP_000818618.1 shikimate kinase AroK -
  KIM20_RS25535 (KIM20_25240) comE 5019443..5020681 (-) 1239 WP_000815968.1 DNA uptake porin HofQ Machinery gene
  KIM20_RS25540 (KIM20_25245) hofP 5020593..5020997 (-) 405 WP_001300917.1 DNA utilization protein HofP -
  KIM20_RS25545 (KIM20_25250) hofO 5020987..5021427 (-) 441 WP_001055754.1 DNA utilization protein HofO -
  KIM20_RS25550 (KIM20_25255) hofN 5021411..5021950 (-) 540 WP_001069315.1 DNA utilization protein HofN -
  KIM20_RS25555 (KIM20_25260) hofM 5021950..5022729 (-) 780 WP_001341921.1 DNA utilization protein HofM -
  KIM20_RS25560 (KIM20_25265) mrcA 5022849..5025401 (+) 2553 WP_000673804.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44774.27 Da        Isoelectric Point: 6.1173

>NTDB_id=494284 KIM20_RS25535 WP_000815968.1 5019443..5020681(-) (comE) [Escherichia coli strain 2013C_3277]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEDNILSVHSIAWQNNNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=494284 KIM20_RS25535 WP_000815968.1 5019443..5020681(-) (comE) [Escherichia coli strain 2013C_3277]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGGCAGGAAGACAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATAACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGCTTGCTGGATCTTGAGCTTTCTGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.385

100

0.396
  comE Haemophilus influenzae 86-028NP

37.156

100

0.393
  pilQ Vibrio campbellii strain DS40M4

37.767

100

0.386
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.288

100

0.374
  pilQ Vibrio cholerae strain A1552

37.288

100

0.374
  comE Glaesserella parasuis strain SC1401

35.952

100

0.367