Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   IM816_RS16155 Genome accession   NZ_CP063231
Coordinates   3711529..3712587 (-) Length   352 a.a.
NCBI ID   WP_072323749.1    Uniprot ID   A0A1K1RNV8
Organism   Luteibacter flocculans strain EIF3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3706529..3717587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IM816_RS16135 (IM816_16105) - 3707319..3709595 (-) 2277 WP_250338862.1 type IV pilus secretin PilQ -
  IM816_RS16140 (IM816_16110) - 3709615..3710130 (-) 516 WP_250338863.1 pilus assembly protein PilP -
  IM816_RS16145 (IM816_16115) - 3710139..3710786 (-) 648 WP_072323747.1 type 4a pilus biogenesis protein PilO -
  IM816_RS16150 (IM816_16120) - 3710783..3711529 (-) 747 WP_250338864.1 PilN domain-containing protein -
  IM816_RS16155 (IM816_16125) pilM 3711529..3712587 (-) 1059 WP_072323749.1 pilus assembly protein PilM Machinery gene
  IM816_RS16160 (IM816_16130) - 3712828..3715401 (+) 2574 WP_250338865.1 penicillin-binding protein 1A -
  IM816_RS16165 (IM816_16135) - 3715461..3716117 (+) 657 WP_250338866.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37879.51 Da        Isoelectric Point: 4.5287

>NTDB_id=493986 IM816_RS16155 WP_072323749.1 3711529..3712587(-) (pilM) [Luteibacter flocculans strain EIF3]
MGLFTPKAAPLIGVDISSTAVKLLQLSQAGGRYRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRALTRSGAKVKHAAAA
VAGSAVITRVIPMSADLSEDDLEGQIQVEANQYIPYPIEEVSLDFEVLGPVRDNPEMNNVLLAASRTENVDMRIAALDLG
GLTARVIDVEAFAMENAFGMVADQLAVSRDALVAVVDIGATMTTLAVLKNQRTIYSREQVFGGKQLTDEIMRRYGLSYEE
AGRAKRKGGLPESYEMEALEPFKESLVQQVSRLLQFFFAGSEYSRVDQVVLAGGCAAIEGITELLEQQLGVPCVVANPLA
RMALSSRVQAQTLAQDAPALMIAVGLAMRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=493986 IM816_RS16155 WP_072323749.1 3711529..3712587(-) (pilM) [Luteibacter flocculans strain EIF3]
GTGGGGCTCTTTACACCCAAAGCGGCGCCGTTGATCGGCGTCGATATCAGCTCGACCGCGGTAAAGCTGCTTCAACTCAG
CCAAGCCGGCGGCCGTTATCGCGTGGAACACTACGCGGTAGAGCCGTTGCCGCCCAATGCGGTCGTCGAAAAGAACATTG
TCGAGGTCGAGGCCGTGGGTGAAGCCATCCGGCGCGCCCTCACGCGGTCCGGCGCCAAGGTGAAGCATGCGGCGGCGGCG
GTAGCCGGCTCCGCGGTGATCACCCGGGTCATCCCCATGTCGGCCGATCTCTCCGAGGACGACCTCGAGGGGCAGATCCA
GGTCGAGGCGAACCAGTACATCCCCTACCCGATCGAGGAAGTGAGCCTCGATTTCGAGGTTCTGGGCCCGGTTCGCGACA
ACCCCGAAATGAACAACGTGCTCCTGGCCGCCTCGCGGACGGAGAACGTGGACATGCGCATCGCCGCGCTCGACCTGGGT
GGCCTGACCGCCCGGGTCATCGATGTCGAGGCATTCGCCATGGAAAACGCCTTCGGCATGGTGGCCGACCAGTTGGCGGT
TTCCCGTGACGCGCTTGTCGCGGTCGTCGACATCGGCGCCACCATGACCACCCTGGCGGTGCTGAAGAACCAGCGCACCA
TCTATTCCCGCGAACAGGTCTTCGGCGGCAAGCAGCTCACCGACGAGATCATGCGCCGCTACGGACTTTCGTACGAGGAA
GCGGGTCGGGCCAAGCGCAAGGGCGGCCTGCCGGAGTCCTACGAGATGGAGGCGCTCGAGCCGTTCAAGGAATCCCTGGT
CCAGCAGGTCAGCCGCCTGCTCCAGTTCTTCTTTGCCGGCAGCGAATACAGCCGCGTCGACCAGGTGGTGCTCGCCGGCG
GCTGCGCTGCCATCGAGGGCATCACCGAACTGCTCGAACAGCAGCTCGGCGTGCCGTGCGTGGTTGCCAATCCGCTGGCG
CGCATGGCGCTGTCCAGCCGCGTACAGGCGCAGACCCTCGCCCAGGACGCGCCCGCCCTGATGATTGCCGTCGGCCTGGC
CATGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1K1RNV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

53.216

97.159

0.517
  comM Acinetobacter nosocomialis M2

52.924

97.159

0.514
  comM Acinetobacter baylyi ADP1

51.585

98.58

0.509
  pilM Legionella pneumophila strain ERS1305867

41.926

100

0.42