Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   KIM35_RS16260 Genome accession   NZ_CP074815
Coordinates   3191185..3192195 (-) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain 7_33_80A     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3186185..3197195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM35_RS16240 (KIM35_16015) mepM 3187152..3188474 (-) 1323 WP_001184054.1 murein DD-endopeptidase MepM -
  KIM35_RS16245 (KIM35_16020) znuA 3188490..3189422 (-) 933 WP_001300644.1 zinc ABC transporter substrate-binding protein ZnuA -
  KIM35_RS16250 (KIM35_16025) znuC 3189501..3190256 (+) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  KIM35_RS16255 (KIM35_16030) znuB 3190253..3191038 (+) 786 WP_000571465.1 zinc ABC transporter permease subunit ZnuB -
  KIM35_RS16260 (KIM35_16035) ruvB 3191185..3192195 (-) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  KIM35_RS16265 (KIM35_16040) ruvA 3192204..3192815 (-) 612 WP_000580328.1 Holliday junction branch migration protein RuvA -
  KIM35_RS16270 (KIM35_16045) yobI 3192954..3193019 (-) 66 WP_001386853.1 stress response small protein YobI -
  KIM35_RS16275 (KIM35_16050) yebB 3193090..3193692 (+) 603 WP_001024917.1 YebB family permuted papain-like enzyme -
  KIM35_RS16280 (KIM35_16055) ruvC 3193694..3194215 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  KIM35_RS16285 (KIM35_16060) yebC 3194250..3194990 (-) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  KIM35_RS16290 (KIM35_16065) nudB 3195019..3195471 (-) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=493063 KIM35_RS16260 WP_000568519.1 3191185..3192195(-) (ruvB) [Escherichia coli strain 7_33_80A]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=493063 KIM35_RS16260 WP_000568519.1 3191185..3192195(-) (ruvB) [Escherichia coli strain 7_33_80A]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTCGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGTTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGAGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494