Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   INP94_RS00260 Genome accession   NZ_CP063120
Coordinates   54570..55949 (+) Length   459 a.a.
NCBI ID   WP_197543655.1    Uniprot ID   A0A7M1NX55
Organism   Haemophilus parainfluenzae strain M1C137_2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 49570..60949
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP94_RS00230 (INP94_00230) - 49861..50712 (-) 852 WP_049364941.1 thymidylate synthase -
  INP94_RS00235 (INP94_00235) lgt 50726..51532 (-) 807 WP_070582537.1 prolipoprotein diacylglyceryl transferase -
  INP94_RS00240 (INP94_00240) - 51541..52335 (-) 795 WP_049371641.1 sulfite exporter TauE/SafE family protein -
  INP94_RS00245 (INP94_00245) rppH 52335..52928 (-) 594 WP_049371640.1 RNA pyrophosphohydrolase -
  INP94_RS00250 (INP94_00250) ampD 53414..53977 (-) 564 WP_049371639.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  INP94_RS00255 (INP94_00255) pilA 54120..54563 (+) 444 WP_005697917.1 prepilin peptidase-dependent pilin Machinery gene
  INP94_RS00260 (INP94_00260) pilB 54570..55949 (+) 1380 WP_197543655.1 GspE/PulE family protein Machinery gene
  INP94_RS00265 (INP94_00265) pilC 55958..57181 (+) 1224 WP_197543656.1 type II secretion system F family protein Machinery gene
  INP94_RS00270 (INP94_00270) pilD 57178..57855 (+) 678 WP_197543657.1 prepilin peptidase Machinery gene
  INP94_RS00275 (INP94_00275) coaE 57944..58564 (+) 621 WP_197543658.1 dephospho-CoA kinase -
  INP94_RS00280 (INP94_00280) yacG 58557..58763 (+) 207 WP_005695505.1 DNA gyrase inhibitor YacG -
  INP94_RS00285 (INP94_00285) - 58760..59041 (+) 282 WP_197543659.1 GNAT family N-acetyltransferase -
  INP94_RS00290 (INP94_00290) rhlB 59132..60343 (+) 1212 WP_197543660.1 ATP-dependent RNA helicase RhlB -
  INP94_RS00295 (INP94_00295) mreD 60432..60920 (-) 489 WP_197543661.1 rod shape-determining protein MreD -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 52153.56 Da        Isoelectric Point: 6.1906

>NTDB_id=493063 INP94_RS00260 WP_197543655.1 54570..55949(+) (pilB) [Haemophilus parainfluenzae strain M1C137_2]
MAYQAIAKNGEIYQISPQYWQQNQQQQALLLRYFALPLKEDEHHLWLAVDSLNNLAACETFAFLSGKLVEPILFETAQLK
QLLQSLAPKANQIEEQTTFYHHSEDENTANLSNEDEPVIQLLNGIFETALQKNASDIHLETQPNGLLIRLRIDGVLQPQP
VISKSLANRVISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKAVLRLQQNKPVQLSFAELGMTQN
QQIQFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPVEIELEGIIQTQVNPQIGLDFSRLLRTFLR
QDPDIIMLGEIRDEESALMALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQSHEIENSLLLVIAQRLVRKRCLKCGQHF
SDSCDCHQGYRGRIGVYQFLQCENNRYQTDFESLYQSALEKVKDRITDLIEIQRVLGKK

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=493063 INP94_RS00260 WP_197543655.1 54570..55949(+) (pilB) [Haemophilus parainfluenzae strain M1C137_2]
ATGGCTTATCAAGCAATCGCAAAAAATGGCGAGATTTATCAGATCTCGCCACAATATTGGCAACAAAACCAACAGCAACA
AGCTTTGCTGTTGCGTTACTTTGCGCTCCCCCTTAAAGAAGACGAACACCATTTATGGCTTGCAGTGGATTCACTCAATA
ATCTTGCCGCCTGTGAAACCTTTGCTTTTTTAAGTGGAAAATTAGTCGAACCGATTTTATTTGAAACGGCCCAACTTAAA
CAACTTTTACAATCCCTTGCACCTAAAGCCAATCAAATAGAAGAGCAAACCACGTTTTATCATCATTCAGAAGACGAAAA
CACGGCTAATTTATCTAATGAAGATGAACCCGTTATTCAATTATTAAATGGTATTTTTGAAACAGCCCTACAAAAAAATG
CCTCCGATATTCATTTAGAAACTCAGCCAAATGGGCTCTTAATTCGTTTACGTATTGATGGTGTGCTCCAACCACAACCG
GTAATCAGTAAATCGCTCGCCAATCGTGTAATTTCTCGTTTAAAACTCTTAGCCAAACTGGATATCAGTGAAACTCGGCT
ACCACAAGATGGCCGCTTTCAATTCAAAACGACATTCTCCGATATTTTAGATTTCCGCCTTTCTACCTTGCCCACGCATT
GGGGAGAAAAAGCGGTATTACGATTACAACAAAATAAACCTGTTCAGTTAAGTTTTGCTGAACTTGGCATGACACAAAAC
CAACAAATCCAATTTCAACATGCCCTCAGCCAACCGCAAGGTTTAATTCTTGTGACTGGACCAACGGGAAGCGGAAAAAG
CATCTCGCTTTATACCGCACTTCAATGGCTCAATACGCCAGATAAACATATTATGACGGCAGAAGATCCCGTTGAAATTG
AATTAGAAGGTATCATTCAAACTCAAGTAAATCCGCAAATCGGTTTAGATTTTAGTCGATTACTTCGTACATTTTTACGA
CAAGACCCCGACATTATTATGCTTGGTGAAATTCGAGATGAAGAAAGTGCATTAATGGCATTAAGAGCCGCTCAAACCGG
CCATTTAGTGCTTTCTACCTTACATACCAACGATGCCATCTCAGCTATTTCACGCTTACAACAACTAGGTATTCAATCTC
ACGAAATTGAAAATAGTCTCTTGTTAGTTATCGCACAAAGATTGGTGCGTAAACGTTGCTTAAAGTGCGGCCAACATTTT
AGTGATTCTTGCGATTGCCATCAAGGATATCGAGGTCGTATTGGTGTGTATCAATTTTTACAATGCGAAAATAACCGTTA
CCAAACTGATTTTGAGTCACTTTATCAAAGTGCGTTAGAAAAAGTAAAAGATCGCATCACCGATCTCATCGAAATTCAAC
GCGTGTTAGGTAAAAAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(125-391)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(25-88)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7M1NX55

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

78.761

98.475

0.776
  pilB Haemophilus influenzae Rd KW20

77.876

98.475

0.767
  pilB Glaesserella parasuis strain SC1401

59.735

98.475

0.588
  pilB Vibrio campbellii strain DS40M4

41.545

100

0.434
  pilB Vibrio cholerae strain A1552

41.25

100

0.431
  pilB Vibrio parahaemolyticus RIMD 2210633

41.042

100

0.429
  pilB Acinetobacter baylyi ADP1

39.034

100

0.423
  pilB Legionella pneumophila strain ERS1305867

38.855

100

0.414
  pilB Acinetobacter baumannii D1279779

39.014

100

0.414
  pilF Neisseria gonorrhoeae MS11

39.161

93.464

0.366
  pilF Thermus thermophilus HB27

38.928

93.464

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.095

100

0.362