Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   KH969_RS11355 Genome accession   NZ_CP074701
Coordinates   2338238..2339731 (-) Length   497 a.a.
NCBI ID   WP_023141654.1    Uniprot ID   -
Organism   Escherichia coli strain 702/18     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2333238..2344731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KH969_RS11325 (KH969_11335) gspK 2334032..2335009 (-) 978 WP_023141656.1 type II secretion system minor pseudopilin GspK -
  KH969_RS11330 (KH969_11340) gspJ 2335006..2335611 (-) 606 WP_001254778.1 type II secretion system minor pseudopilin GspJ -
  KH969_RS11335 (KH969_11345) gspI 2335608..2335979 (-) 372 WP_000820092.1 type II secretion system minor pseudopilin GspI -
  KH969_RS11340 (KH969_11350) gspH 2335976..2336539 (-) 564 WP_024175821.1 type II secretion system minor pseudopilin GspH -
  KH969_RS11345 (KH969_11355) gspG 2336543..2336998 (-) 456 WP_001087298.1 type II secretion system major pseudopilin GspG -
  KH969_RS11350 (KH969_11360) gspF 2337015..2338238 (-) 1224 WP_001173427.1 type II secretion system inner membrane protein GspF -
  KH969_RS11355 (KH969_11365) pilB/pilB1 2338238..2339731 (-) 1494 WP_023141654.1 type II secretion system ATPase GspE Machinery gene
  KH969_RS11360 (KH969_11370) gspD 2339731..2341791 (-) 2061 WP_000498848.1 type II secretion system secretin GspD -
  KH969_RS11365 (KH969_11375) gspC 2341821..2342780 (-) 960 WP_023141653.1 type II secretion system protein GspC -
  KH969_RS11370 (KH969_11380) gspS2 2342798..2343208 (-) 411 WP_001324279.1 type II secretion system pilot lipoprotein GspS-beta -
  KH969_RS11375 (KH969_11385) pilD 2343274..2344083 (-) 810 WP_001296399.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55163.23 Da        Isoelectric Point: 5.9800

>NTDB_id=492011 KH969_RS11355 WP_023141654.1 2338238..2339731(-) (pilB/pilB1) [Escherichia coli strain 702/18]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQAAPA
TDEEKRLMGISDTHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=492011 KH969_RS11355 WP_023141654.1 2338238..2339731(-) (pilB/pilB1) [Escherichia coli strain 702/18]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGTCTTTTACGCTGAGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAACTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACCCTCGCAGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGCAAGCTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGGCTGGATATCGCCGAAAAGCGCGTACC
ACAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATCGACGTGCGCGTCTCCACCATGCCTTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACACTGGAGCGTCTGGGTTTAAGTCACGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACAGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGATCCCATCGAATACATGATTG
AAGGGATCGGTCAGACACAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCGTCGCTCACGGGGCACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACACGTCTGCAGGATATGGGCGTTGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGGCTGGTTCGCACGCTGTGTCCCGACTGCCGCCAGGCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTTATGGGGATCTCCGATACGCATGCCGTCACGCTGTACCATCCGCAGGGTTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGACGCCACATTACGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAGCGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.926

100

0.435
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.294

85.513

0.404
  pilB Legionella pneumophila strain ERS1305867

41.97

93.964

0.394
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

44.75

80.483

0.36