Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IHV08_RS13035 Genome accession   NZ_CP062497
Coordinates   2507018..2508088 (-) Length   356 a.a.
NCBI ID   WP_181217184.1    Uniprot ID   -
Organism   Bacillus subtilis strain CV16     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2502018..2513088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHV08_RS12990 (IHV08_12990) tapA 2502497..2503258 (-) 762 WP_181217179.1 amyloid fiber anchoring/assembly protein TapA -
  IHV08_RS12995 (IHV08_12995) yqzG 2503530..2503856 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  IHV08_RS13000 (IHV08_13000) spoIITA 2503898..2504077 (-) 180 WP_003230176.1 YqzE family protein -
  IHV08_RS13005 (IHV08_13005) comGG 2504148..2504522 (-) 375 WP_015251714.1 ComG operon protein ComGG Machinery gene
  IHV08_RS13010 (IHV08_13010) comGF 2504523..2504906 (-) 384 WP_015251713.1 ComG operon protein ComGF Machinery gene
  IHV08_RS13015 (IHV08_13015) comGE 2504932..2505279 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  IHV08_RS13020 (IHV08_13020) comGD 2505263..2505694 (-) 432 WP_181217182.1 comG operon protein ComGD Machinery gene
  IHV08_RS13025 (IHV08_13025) comGC 2505684..2505980 (-) 297 WP_014477332.1 comG operon protein ComGC Machinery gene
  IHV08_RS13030 (IHV08_13030) comGB 2505994..2507031 (-) 1038 WP_181217183.1 comG operon protein ComGB Machinery gene
  IHV08_RS13035 (IHV08_13035) comGA 2507018..2508088 (-) 1071 WP_181217184.1 competence protein ComGA Machinery gene
  IHV08_RS13040 (IHV08_13040) corA 2508500..2509453 (-) 954 WP_181217185.1 magnesium transporter CorA -
  IHV08_RS13045 (IHV08_13045) yqhB 2509596..2510924 (+) 1329 WP_181217186.1 hemolysin family protein -
  IHV08_RS13050 (IHV08_13050) rsbRD 2510977..2511813 (-) 837 WP_173614187.1 RsbT co-antagonist protein RsbRD -
  IHV08_RS13060 (IHV08_13060) mgsR 2512037..2512417 (-) 381 WP_014477337.1 transcriptional regulator MgsR -
  IHV08_RS13065 (IHV08_13065) yqgY 2512649..2512894 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40487.68 Da        Isoelectric Point: 9.1812

>NTDB_id=490457 IHV08_RS13035 WP_181217184.1 2507018..2508088(-) (comGA) [Bacillus subtilis strain CV16]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSTGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIQQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=490457 IHV08_RS13035 WP_181217184.1 2507018..2508088(-) (comGA) [Bacillus subtilis strain CV16]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTAAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGACCAACTGGTTCAGGGAAGACTACCACACTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCACAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCCAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTTTACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGGAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

99.438

100

0.994
  pilF Thermus thermophilus HB27

36.827

99.157

0.365
  pilB Glaesserella parasuis strain SC1401

43.878

82.584

0.362