Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   IHV08_RS01965 Genome accession   NZ_CP062497
Coordinates   364077..364526 (-) Length   149 a.a.
NCBI ID   WP_033883649.1    Uniprot ID   -
Organism   Bacillus subtilis strain CV16     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 359077..369526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHV08_RS01925 (IHV08_01925) - 359511..359708 (+) 198 Protein_335 hypothetical protein -
  IHV08_RS01930 (IHV08_01930) - 359745..360065 (+) 321 Protein_336 RDD family protein -
  IHV08_RS01935 (IHV08_01935) yckD 360147..360479 (+) 333 WP_015252849.1 YckD family protein -
  IHV08_RS01940 (IHV08_01940) - 360607..361029 (+) 423 WP_174616579.1 MarR family transcriptional regulator -
  IHV08_RS01945 (IHV08_01945) - 361017..361358 (+) 342 WP_192858187.1 DUF3147 family protein -
  IHV08_RS01950 (IHV08_01950) - 361377..362109 (+) 733 Protein_340 AAA family ATPase -
  IHV08_RS01955 (IHV08_01955) bglC 362182..363615 (+) 1434 WP_192858188.1 6-phospho-beta-glucosidase -
  IHV08_RS01960 (IHV08_01960) nin/comJ 363652..364050 (-) 399 WP_015252845.1 DNA-entry nuclease inhibitor Regulator
  IHV08_RS01965 (IHV08_01965) nucA/comI 364077..364526 (-) 450 WP_033883649.1 DNA-entry nuclease Machinery gene
  IHV08_RS01970 (IHV08_01970) tlpC 364694..366415 (-) 1722 WP_192858189.1 methyl-accepting chemotaxis protein TlpC -
  IHV08_RS01975 (IHV08_01975) hxlB 366526..367083 (-) 558 WP_003246343.1 6-phospho-3-hexuloisomerase -
  IHV08_RS01980 (IHV08_01980) hxlA 367089..367721 (-) 633 WP_192858190.1 3-hexulose-6-phosphate synthase -
  IHV08_RS01985 (IHV08_01985) hxlR 367953..368315 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16454.42 Da        Isoelectric Point: 4.5282

>NTDB_id=490416 IHV08_RS01965 WP_033883649.1 364077..364526(-) (nucA/comI) [Bacillus subtilis strain CV16]
MNITTDIIKTILLVIVIIAAAAIGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=490416 IHV08_RS01965 WP_033883649.1 364077..364526(-) (nucA/comI) [Bacillus subtilis strain CV16]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTATCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCTTCTGACCGTT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98