Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   ILP75_RS02345 Genome accession   NZ_CP062471
Coordinates   493496..493993 (-) Length   165 a.a.
NCBI ID   WP_001829055.1    Uniprot ID   -
Organism   Staphylococcus aureus strain MRSA - AMRF 3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 488496..498993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ILP75_RS02320 (ILP75_02320) gcvPB 488658..490130 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  ILP75_RS02325 (ILP75_02325) gcvPA 490123..491469 (-) 1347 WP_194085842.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  ILP75_RS02330 (ILP75_02330) gcvT 491489..492580 (-) 1092 WP_000093346.1 glycine cleavage system aminomethyltransferase GcvT -
  ILP75_RS02335 (ILP75_02335) - 492739..493263 (-) 525 WP_001015121.1 shikimate kinase -
  ILP75_RS02340 (ILP75_02340) - 493253..493399 (-) 147 WP_001789879.1 hypothetical protein -
  ILP75_RS02345 (ILP75_02345) comGF 493496..493993 (-) 498 WP_001829055.1 competence type IV pilus minor pilin ComGF Machinery gene
  ILP75_RS02350 (ILP75_02350) comGE 493911..494210 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  ILP75_RS02355 (ILP75_02355) comGD 494197..494643 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  ILP75_RS02360 (ILP75_02360) comGC 494621..494932 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  ILP75_RS02365 (ILP75_02365) comGB 494946..496016 (-) 1071 WP_000776406.1 competence type IV pilus assembly protein ComGB Machinery gene
  ILP75_RS02370 (ILP75_02370) comGA 495988..496962 (-) 975 WP_000697227.1 competence type IV pilus ATPase ComGA Machinery gene
  ILP75_RS02375 (ILP75_02375) - 497014..497637 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  ILP75_RS02380 (ILP75_02380) - 497634..497963 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ILP75_RS02385 (ILP75_02385) - 497963..498949 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19183.92 Da        Isoelectric Point: 10.2192

>NTDB_id=490317 ILP75_RS02345 WP_001829055.1 493496..493993(-) (comGF) [Staphylococcus aureus strain MRSA - AMRF 3]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=490317 ILP75_RS02345 WP_001829055.1 493496..493993(-) (comGF) [Staphylococcus aureus strain MRSA - AMRF 3]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

98.182

100

0.982