Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   KFU76_RS16360 Genome accession   NZ_CP074028
Coordinates   3411379..3412617 (+) Length   412 a.a.
NCBI ID   WP_106661443.1    Uniprot ID   -
Organism   Escherichia coli strain PK13     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3406379..3417617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU76_RS16335 (KFU76_16200) mrcA 3406659..3409211 (-) 2553 WP_001367029.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  KFU76_RS16340 (KFU76_16205) hofM 3409331..3410110 (+) 780 WP_001315880.1 DNA utilization protein HofM -
  KFU76_RS16345 (KFU76_16210) hofN 3410110..3410649 (+) 540 WP_001069315.1 DNA utilization protein HofN -
  KFU76_RS16350 (KFU76_16215) hofO 3410633..3411073 (+) 441 WP_001055760.1 DNA utilization protein HofO -
  KFU76_RS16355 (KFU76_16220) hofP 3411063..3411467 (+) 405 WP_001264141.1 DNA utilization protein HofP -
  KFU76_RS16360 (KFU76_16225) comE 3411379..3412617 (+) 1239 WP_106661443.1 DNA uptake porin HofQ Machinery gene
  KFU76_RS16365 (KFU76_16230) aroK 3413018..3413539 (+) 522 WP_000818618.1 shikimate kinase AroK -
  KFU76_RS16370 (KFU76_16235) aroB 3413596..3414684 (+) 1089 WP_000439846.1 3-dehydroquinate synthase -
  KFU76_RS16375 (KFU76_16240) damX 3414776..3416062 (+) 1287 WP_000343193.1 cell division protein DamX -
  KFU76_RS16380 (KFU76_16245) dam 3416169..3417005 (+) 837 WP_000742143.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44664.17 Da        Isoelectric Point: 5.8991

>NTDB_id=490148 KFU76_RS16360 WP_106661443.1 3411379..3412617(+) (comE) [Escherichia coli strain PK13]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNDNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKCTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=490148 KFU76_RS16360 WP_106661443.1 3411379..3412617(+) (comE) [Escherichia coli strain PK13]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTGGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGGCTG
ATAACGCGCCAGGAGGGCAACATTCTCTCAGTGCATTCTATTGCCTGGCAGAATGACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAATGCACCAATCGCCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTTCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACACGCTGGTGGTGTTG
GGCAAGTCACCACGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGCTTGCTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

36.927

100

0.391
  pilQ Vibrio campbellii strain DS40M4

38.005

100

0.388
  comE Haemophilus influenzae 86-028NP

36.697

100

0.388
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.53

100

0.376
  pilQ Vibrio cholerae strain A1552

37.53

100

0.376
  comE Glaesserella parasuis strain SC1401

35.714

100

0.364