Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   IF751_RS13405 Genome accession   NZ_CP062407
Coordinates   2740890..2741387 (+) Length   165 a.a.
NCBI ID   WP_020977296.1    Uniprot ID   -
Organism   Staphylococcus aureus strain NAS_NP_181     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2735890..2746387
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF751_RS13365 (IF751_13275) - 2735934..2736920 (+) 987 WP_000161315.1 ROK family glucokinase -
  IF751_RS13370 (IF751_13280) - 2736920..2737249 (+) 330 WP_020977297.1 MTH1187 family thiamine-binding protein -
  IF751_RS13375 (IF751_13285) - 2737246..2737869 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  IF751_RS13380 (IF751_13290) comGA 2737921..2738895 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  IF751_RS13385 (IF751_13295) comGB 2738867..2739937 (+) 1071 WP_000776407.1 competence type IV pilus assembly protein ComGB Machinery gene
  IF751_RS13390 (IF751_13300) comGC 2739951..2740262 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  IF751_RS13395 (IF751_13305) comGD 2740240..2740686 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  IF751_RS13400 (IF751_13310) comGE 2740673..2740972 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  IF751_RS13405 (IF751_13315) comGF 2740890..2741387 (+) 498 WP_020977296.1 competence type IV pilus minor pilin ComGF Machinery gene
  IF751_RS13410 (IF751_13320) - 2741484..2741630 (+) 147 WP_001789879.1 hypothetical protein -
  IF751_RS13415 (IF751_13325) - 2741620..2742144 (+) 525 WP_020977295.1 shikimate kinase -
  IF751_RS13420 (IF751_13330) gcvT 2742303..2743394 (+) 1092 WP_020977294.1 glycine cleavage system aminomethyltransferase GcvT -
  IF751_RS13425 (IF751_13335) gcvPA 2743414..2744760 (+) 1347 WP_020977293.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  IF751_RS13430 (IF751_13340) gcvPB 2744753..2746225 (+) 1473 WP_000202177.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19183.92 Da        Isoelectric Point: 10.2192

>NTDB_id=489547 IF751_RS13405 WP_020977296.1 2740890..2741387(+) (comGF) [Staphylococcus aureus strain NAS_NP_181]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNNIEIREQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=489547 IF751_RS13405 WP_020977296.1 2740890..2741387(+) (comGF) [Staphylococcus aureus strain NAS_NP_181]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACAATATTGAAATTAGGGAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(69-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

96.97

100

0.97
  comGF Staphylococcus aureus N315

96.97

100

0.97