Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IF751_RS13380 Genome accession   NZ_CP062407
Coordinates   2737921..2738895 (+) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain NAS_NP_181     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2732921..2743895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF751_RS13345 (IF751_13255) rpmG 2733381..2733530 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  IF751_RS13350 (IF751_13260) - 2733739..2734278 (+) 540 WP_042908703.1 5-formyltetrahydrofolate cyclo-ligase -
  IF751_RS13355 (IF751_13265) - 2734290..2735753 (+) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  IF751_RS13360 (IF751_13270) - 2735734..2735937 (+) 204 WP_000087561.1 YqgQ family protein -
  IF751_RS13365 (IF751_13275) - 2735934..2736920 (+) 987 WP_000161315.1 ROK family glucokinase -
  IF751_RS13370 (IF751_13280) - 2736920..2737249 (+) 330 WP_020977297.1 MTH1187 family thiamine-binding protein -
  IF751_RS13375 (IF751_13285) - 2737246..2737869 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  IF751_RS13380 (IF751_13290) comGA 2737921..2738895 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  IF751_RS13385 (IF751_13295) comGB 2738867..2739937 (+) 1071 WP_000776407.1 competence type IV pilus assembly protein ComGB Machinery gene
  IF751_RS13390 (IF751_13300) comGC 2739951..2740262 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  IF751_RS13395 (IF751_13305) comGD 2740240..2740686 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  IF751_RS13400 (IF751_13310) comGE 2740673..2740972 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  IF751_RS13405 (IF751_13315) comGF 2740890..2741387 (+) 498 WP_020977296.1 competence type IV pilus minor pilin ComGF Machinery gene
  IF751_RS13410 (IF751_13320) - 2741484..2741630 (+) 147 WP_001789879.1 hypothetical protein -
  IF751_RS13415 (IF751_13325) - 2741620..2742144 (+) 525 WP_020977295.1 shikimate kinase -
  IF751_RS13420 (IF751_13330) gcvT 2742303..2743394 (+) 1092 WP_020977294.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=489542 IF751_RS13380 WP_000697220.1 2737921..2738895(+) (comGA) [Staphylococcus aureus strain NAS_NP_181]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=489542 IF751_RS13380 WP_000697220.1 2737921..2738895(+) (comGA) [Staphylococcus aureus strain NAS_NP_181]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCCTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTAATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAGGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1