Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   KD154_RS13690 Genome accession   NZ_CP073589
Coordinates   2784823..2786061 (-) Length   412 a.a.
NCBI ID   WP_072158970.1    Uniprot ID   -
Organism   Escherichia coli strain MFDS1006657     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2779823..2791061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KD154_RS13670 dam 2780435..2781271 (-) 837 WP_000742143.1 adenine-specific DNA-methyltransferase -
  KD154_RS13675 damX 2781378..2782664 (-) 1287 WP_000343193.1 cell division protein DamX -
  KD154_RS13680 aroB 2782756..2783844 (-) 1089 WP_000439846.1 3-dehydroquinate synthase -
  KD154_RS13685 aroK 2783901..2784422 (-) 522 WP_000818618.1 shikimate kinase AroK -
  KD154_RS13690 comE 2784823..2786061 (-) 1239 WP_072158970.1 DNA uptake porin HofQ Machinery gene
  KD154_RS13695 hofP 2785973..2786377 (-) 405 WP_001264141.1 DNA utilization protein HofP -
  KD154_RS13700 hofO 2786367..2786807 (-) 441 WP_001055753.1 DNA utilization protein HofO -
  KD154_RS13705 hofN 2786791..2787330 (-) 540 WP_001052913.1 DNA utilization protein HofN -
  KD154_RS13710 hofM 2787330..2788109 (-) 780 WP_001349954.1 DNA utilization protein HofM -
  KD154_RS13715 mrcA 2788229..2790781 (+) 2553 WP_000673804.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44804.36 Da        Isoelectric Point: 6.1173

>NTDB_id=487354 KD154_RS13690 WP_072158970.1 2784823..2786061(-) (comE) [Escherichia coli strain MFDS1006657]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEDNILSVHSIAWQNNNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGEMLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=487354 KD154_RS13690 WP_072158970.1 2784823..2786061(-) (comE) [Escherichia coli strain MFDS1006657]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGGCAGGAAGACAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATAACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGCTTGCTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAATGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  comE Haemophilus influenzae 86-028NP

36.927

100

0.391
  pilQ Vibrio campbellii strain DS40M4

37.53

100

0.383
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.046

100

0.371
  pilQ Vibrio cholerae strain A1552

37.046

100

0.371
  comE Glaesserella parasuis strain SC1401

35.714

100

0.364