Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   JNUCC41_RS25940 Genome accession   NZ_CP062163
Coordinates   5369881..5370957 (-) Length   358 a.a.
NCBI ID   WP_192208340.1    Uniprot ID   -
Organism   Brevibacillus sp. JNUCC-41     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5364881..5375957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNUCC41_RS25900 (JNUCC41_25900) - 5365746..5366546 (+) 801 WP_192205487.1 YqhG family protein -
  JNUCC41_RS25905 (JNUCC41_25905) - 5366590..5366775 (-) 186 WP_063233067.1 YqzE family protein -
  JNUCC41_RS25910 (JNUCC41_25910) comGG 5366892..5367278 (-) 387 WP_192205490.1 competence type IV pilus minor pilin ComGG -
  JNUCC41_RS25915 (JNUCC41_25915) comGF 5367271..5367750 (-) 480 WP_192208338.1 competence type IV pilus minor pilin ComGF -
  JNUCC41_RS25920 (JNUCC41_25920) - 5367698..5368036 (-) 339 WP_192205492.1 hypothetical protein -
  JNUCC41_RS25925 (JNUCC41_25925) comGD 5368023..5368466 (-) 444 WP_192205494.1 competence type IV pilus minor pilin ComGD -
  JNUCC41_RS25930 (JNUCC41_25930) comGC 5368474..5368773 (-) 300 WP_286182430.1 competence type IV pilus major pilin ComGC Machinery gene
  JNUCC41_RS25935 (JNUCC41_25935) comGB 5368863..5369897 (-) 1035 WP_192205496.1 competence type IV pilus assembly protein ComGB -
  JNUCC41_RS25940 (JNUCC41_25940) comGA 5369881..5370957 (-) 1077 WP_192208340.1 competence type IV pilus ATPase ComGA Machinery gene
  JNUCC41_RS25945 (JNUCC41_25945) - 5371059..5371439 (-) 381 WP_192205498.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  JNUCC41_RS25950 (JNUCC41_25950) - 5371655..5372350 (+) 696 WP_192205500.1 helix-turn-helix domain-containing protein -
  JNUCC41_RS25955 (JNUCC41_25955) - 5372436..5372678 (+) 243 WP_076365317.1 DUF2626 domain-containing protein -
  JNUCC41_RS25960 (JNUCC41_25960) - 5372734..5373852 (-) 1119 WP_192205502.1 SAM-dependent methyltransferase -
  JNUCC41_RS25965 (JNUCC41_25965) - 5374228..5374854 (-) 627 WP_192205504.1 MBL fold metallo-hydrolase -
  JNUCC41_RS25970 (JNUCC41_25970) - 5375092..5375265 (+) 174 WP_192205506.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39739.31 Da        Isoelectric Point: 8.1659

>NTDB_id=486686 JNUCC41_RS25940 WP_192208340.1 5369881..5370957(-) (comGA) [Brevibacillus sp. JNUCC-41]
MISIEKTAEKILTRAVQESASDIHIFFRREGPLIQFRIDNKLVPKETLSFFEAERLIAHLKFLASMDIGEKRRPQSGAIT
IHLANQVVGLRLSTLPTAHLESLVIRLIPQQNILPLEQLSLFPSTVQKLIALLKHSHGMLIFTGPTGSGKTTTLYSLLHH
ANEMINRNIITLEDPIENVSEKVLQVQINEKAGITYSVGLKAVLRHDPDVIMVGEVRDAETAKIAVRAALTGHLVLTTMH
TRDAQGAISRLLEFGVSLLEVEQSLIGVTAQRLVELRCPPCKGDCALPCKMTARNKRASVYELLYGKSLAEVLGIMGDEK
GKATVSYRQLKDEIGKAVAMGYVDSEEYERLVYDETKK

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=486686 JNUCC41_RS25940 WP_192208340.1 5369881..5370957(-) (comGA) [Brevibacillus sp. JNUCC-41]
TTGATATCGATTGAAAAGACCGCAGAAAAAATATTGACCCGTGCTGTCCAGGAATCGGCATCGGATATCCACATTTTTTT
TCGAAGGGAGGGACCTCTCATCCAATTCAGGATAGACAATAAGCTTGTTCCAAAGGAAACCTTATCATTCTTTGAAGCAG
AGAGGCTGATCGCTCATTTGAAGTTCCTTGCCTCGATGGATATAGGGGAGAAAAGGAGGCCCCAAAGTGGTGCCATCACC
ATCCATTTGGCCAACCAGGTGGTCGGACTCCGCCTTTCCACTTTACCCACTGCCCATCTCGAAAGTTTGGTCATCCGCTT
AATCCCCCAACAGAATATCCTTCCTCTAGAACAGTTATCCTTATTTCCAAGCACCGTTCAAAAATTGATTGCGCTCCTGA
AGCATTCCCATGGCATGCTCATATTTACCGGCCCGACCGGCAGTGGAAAAACCACGACACTATATTCCCTGCTTCACCAT
GCCAATGAGATGATCAATCGAAATATTATTACACTTGAAGATCCCATTGAAAATGTATCCGAAAAAGTATTGCAGGTCCA
AATCAATGAAAAGGCGGGCATTACGTATTCTGTCGGTCTAAAAGCTGTTCTGAGGCATGACCCAGACGTGATCATGGTTG
GGGAAGTCAGGGATGCAGAAACCGCCAAGATCGCAGTGCGTGCCGCATTGACCGGTCATTTGGTACTTACAACCATGCAT
ACAAGGGACGCCCAGGGCGCCATCTCCAGGTTACTGGAATTTGGTGTCAGCTTGCTTGAGGTTGAGCAGAGTTTGATTGG
CGTGACAGCACAGCGGCTGGTTGAATTGCGATGTCCTCCATGTAAAGGGGACTGTGCTTTACCTTGCAAAATGACTGCCA
GGAATAAAAGGGCAAGTGTATATGAATTGCTATATGGTAAAAGCCTAGCTGAGGTTCTCGGGATAATGGGAGATGAAAAA
GGAAAGGCAACGGTCAGCTACCGCCAATTGAAGGATGAAATCGGAAAAGCGGTTGCGATGGGCTATGTGGATTCCGAGGA
ATATGAACGGCTGGTATATGATGAAACCAAAAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.303

99.721

0.561
  pilB Haemophilus influenzae 86-028NP

39.368

97.207

0.383
  pilB Haemophilus influenzae Rd KW20

37.931

97.207

0.369