Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   IEM32_RS25765 Genome accession   NZ_CP062160
Coordinates   5217191..5218315 (-) Length   374 a.a.
NCBI ID   WP_000228544.1    Uniprot ID   A0A9P2PX49
Organism   Escherichia coli strain 20R2R     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 5212191..5223315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IEM32_RS25735 (IEM32_25735) yrdA 5213991..5214545 (+) 555 WP_001286216.1 gamma carbonic anhydrase family protein -
  IEM32_RS25740 (IEM32_25740) yrdB 5214521..5214778 (-) 258 WP_001070590.1 DUF1488 domain-containing protein -
  IEM32_RS25745 (IEM32_25745) aroE 5214775..5215593 (-) 819 WP_000451254.1 shikimate dehydrogenase -
  IEM32_RS25750 (IEM32_25750) tsaC 5215598..5216170 (-) 573 WP_001301437.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  IEM32_RS25755 (IEM32_25755) yrdD 5216175..5216717 (-) 543 WP_001129722.1 type I DNA topoisomerase -
  IEM32_RS25760 (IEM32_25760) smg 5216746..5217219 (-) 474 WP_000460672.1 DUF494 family protein Smg -
  IEM32_RS25765 (IEM32_25765) dprA 5217191..5218315 (-) 1125 WP_000228544.1 DNA-protecting protein DprA Machinery gene
  IEM32_RS25770 (IEM32_25770) def 5218445..5218954 (+) 510 WP_000114984.1 peptide deformylase -
  IEM32_RS25775 (IEM32_25775) fmt 5218969..5219916 (+) 948 WP_000004459.1 methionyl-tRNA formyltransferase -
  IEM32_RS25780 (IEM32_25780) rsmB 5219962..5221251 (+) 1290 WP_001301795.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  IEM32_RS25785 (IEM32_25785) trkA 5221273..5222649 (+) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  IEM32_RS25790 (IEM32_25790) mscL 5222779..5223189 (+) 411 WP_000022442.1 large-conductance mechanosensitive channel protein MscL -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41005.96 Da        Isoelectric Point: 6.2655

>NTDB_id=486601 IEM32_RS25765 WP_000228544.1 5217191..5218315(-) (dprA) [Escherichia coli strain 20R2R]
MVDTEIWLRLMSISSLYGDDMVRIAHWLARQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=486601 IEM32_RS25765 WP_000228544.1 5217191..5218315(-) (dprA) [Escherichia coli strain 20R2R]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAGACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGGAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGTGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGAAGCATGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTAGCGCATAAAGCAGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCT
TGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGTAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGAGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.78

98.663

0.481
  dprA Glaesserella parasuis strain SC1401

45.401

90.107

0.409
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Haemophilus influenzae Rd KW20

44.214

90.107

0.398
  dprA Legionella pneumophila strain ERS1305867

44.545

88.235

0.393