Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H7691_RS14545 Genome accession   NZ_CP062156
Coordinates   3121364..3123094 (-) Length   576 a.a.
NCBI ID   WP_056931341.1    Uniprot ID   -
Organism   Stenotrophomonas sp. CW117     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3123480..3135827 3121364..3123094 flank 386


Gene organization within MGE regions


Location: 3121364..3135827
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7691_RS14545 (H7691_14545) pilB 3121364..3123094 (-) 1731 WP_056931341.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H7691_RS14550 (H7691_14550) - 3123229..3123480 (+) 252 WP_056931239.1 type II toxin-antitoxin system CcdA family antitoxin -
  H7691_RS14555 (H7691_14555) - 3123480..3123791 (+) 312 WP_056931238.1 CcdB family protein -
  H7691_RS14560 (H7691_14560) - 3123886..3124584 (+) 699 WP_056931237.1 hypothetical protein -
  H7691_RS14565 (H7691_14565) galE 3124606..3125583 (-) 978 WP_056931236.1 UDP-glucose 4-epimerase GalE -
  H7691_RS14570 (H7691_14570) - 3125608..3127500 (-) 1893 WP_152984953.1 hypothetical protein -
  H7691_RS14575 (H7691_14575) - 3127497..3129323 (-) 1827 WP_152984952.1 hypothetical protein -
  H7691_RS14580 (H7691_14580) - 3129282..3130988 (+) 1707 WP_152984951.1 hypothetical protein -
  H7691_RS14585 (H7691_14585) - 3131052..3132584 (-) 1533 WP_056931232.1 NAD(P)/FAD-dependent oxidoreductase -
  H7691_RS14590 (H7691_14590) - 3132587..3133027 (-) 441 WP_056931231.1 GtrA family protein -
  H7691_RS14595 (H7691_14595) - 3133024..3134892 (-) 1869 WP_152984950.1 hypothetical protein -
  H7691_RS19110 (H7691_14600) - 3135007..3135339 (-) 333 WP_082594978.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  H7691_RS18990 - 3135735..3135827 (-) 93 Protein_2861 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62521.76 Da        Isoelectric Point: 5.8299

>NTDB_id=486545 H7691_RS14545 WP_056931341.1 3121364..3123094(-) (pilB) [Stenotrophomonas sp. CW117]
MNTAVTANLVGITGIARRLVQDGAMDEAAARNAMAQAGTAKVPLAQWLAYKKLVNAAQLAAANAVEFGMPLLDTSVFDAG
QNAMKLVSEELLRKHNVLPLFKRGGKLFIGTSNPTQSLDEIKFHTNLVVEPILVDEDQIRRTLDQWVASNESLAGGLGDD
EEGMGDLEVAAGDEDMSGGGDAGIDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRFRIDGLLKTVARAPVK
LRERIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPEQQRLF
LEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGQCKRRAELPAN
ALLAEGFTQAQIDKGIQLYEPVGCEECTEGYKGRTGIYQVMPMTDEIATIVLQGGNALDIAEAAQKIGVNDLRQSALLKA
AQGITSLTEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=486545 H7691_RS14545 WP_056931341.1 3121364..3123094(-) (pilB) [Stenotrophomonas sp. CW117]
ATGAATACAGCCGTCACCGCCAACCTCGTAGGCATCACCGGGATCGCACGCCGCCTGGTGCAGGACGGGGCAATGGACGA
GGCGGCGGCGCGCAACGCGATGGCGCAGGCGGGAACGGCCAAGGTGCCGCTGGCGCAGTGGCTGGCCTATAAGAAGCTGG
TCAACGCCGCGCAGCTGGCGGCGGCCAACGCCGTCGAGTTCGGCATGCCGCTGCTGGATACCTCGGTGTTCGATGCCGGC
CAGAACGCGATGAAGCTGGTCAGCGAGGAACTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAACGTGGCGGCAAGCT
GTTCATCGGCACCAGCAATCCGACCCAGTCGCTGGACGAGATCAAGTTCCACACCAACCTGGTGGTCGAGCCGATCCTGG
TGGACGAGGACCAGATCCGGCGCACGCTGGACCAGTGGGTGGCGAGCAACGAGAGCCTGGCCGGCGGCCTGGGCGACGAC
GAAGAGGGCATGGGCGACCTGGAGGTCGCCGCCGGCGACGAGGACATGAGCGGCGGCGGCGATGCCGGTATCGACGCCAA
GGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGACGCCATCCGCCGCGGCGCCTCGGACATCCACT
TCGAGCCCTACGAGGACGACTATCGGGTGCGCTTCCGCATCGACGGCCTGCTCAAGACCGTGGCCAGGGCGCCGGTGAAG
CTGCGCGAGCGCATCGCCGCGCGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGACGGCCG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACGCTGCCCACGCTGTTCGGCGAGAAGATCG
TGCTGCGCATCCTCGACGGCAGCGCGGCCAAGCTCGGCATCGACAAGCTCGGCTACGAGCCCGAGCAGCAGCGGCTGTTC
CTGGAAGCCATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCCACCGGCTCGGGCAAGACGGTGTCGCTGTATAC
CGCGCTGGGCATCCTCAACGACGAGACCCGCAACATCTCCACTGCCGAGGACCCGGTGGAAATCCGCCTGCCCGGCGTGA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGACCCGGACGTG
ATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGCCATCAAGGCGGCGCAGACCGGCCACATGGTGCTGTC
CACGCTGCATACCAACGACGCACCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCCCCGTACAACATCACCTCCT
CGGTCACCCTGGTCATCGCCCAGCGCCTGGCGCGGCGGTTGTGCGGCCAGTGCAAGCGCCGCGCCGAGCTGCCGGCCAAC
GCGCTGCTGGCCGAAGGCTTCACCCAGGCGCAGATCGACAAGGGCATCCAGCTCTACGAACCGGTCGGCTGCGAGGAGTG
CACCGAAGGCTACAAGGGGCGTACCGGCATCTACCAGGTGATGCCCATGACCGACGAGATCGCCACGATCGTGCTGCAGG
GCGGCAACGCGCTGGACATCGCCGAGGCCGCGCAGAAGATCGGCGTCAACGACCTGCGCCAGTCGGCCCTGCTCAAGGCC
GCGCAGGGCATCACCAGCCTGACCGAGATCAACCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.81

98.611

0.55
  pilB Acinetobacter baumannii D1279779

55.36

98.785

0.547
  pilB Legionella pneumophila strain ERS1305867

53.065

99.132

0.526
  pilB Vibrio cholerae strain A1552

49.215

99.479

0.49
  pilF Neisseria gonorrhoeae MS11

49.474

98.958

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

46.975

97.569

0.458
  pilB Vibrio campbellii strain DS40M4

45.122

99.653

0.45