Detailed information NTG0048635

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NCGM2_RS28690
• Genome accession: NC_017549
• Coordinates: 6191736..6192884 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa NCGM2.S1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6186736..6197884

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NCGM2_RS28670 (NCGM2_5798)	-	6187473..6188657 (-)	1185	WP_003084564.1	cystathionine gamma-synthase	-
NCGM2_RS28675 (NCGM2_5799)	-	6188654..6190027 (-)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
NCGM2_RS28680 (NCGM2_5800)	-	6190270..6190674 (-)	405	WP_003084560.1	NINE protein	-
NCGM2_RS28685 (NCGM2_5801)	czcD	6190829..6191728 (+)	900	WP_003084557.1	CDF family zinc transporter CzcD	-
NCGM2_RS28690 (NCGM2_5802)	pilU	6191736..6192884 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
NCGM2_RS28695 (NCGM2_5803)	pilT	6193062..6194096 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
NCGM2_RS28700 (NCGM2_5804)	-	6194311..6195003 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
NCGM2_RS28705 (NCGM2_5805)	proC	6195015..6195836 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
NCGM2_RS28710 (NCGM2_5806)	-	6195847..6196440 (+)	594	WP_003084544.1	YggT family protein	-
NCGM2_RS28715	-	6196437..6196733 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=48635 NCGM2_RS28690 WP_003100959.1 6191736..6192884(-) (pilU) [Pseudomonas aeruginosa NCGM2.S1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=48635 NCGM2_RS28690 WP_003100959.1 6191736..6192884(-) (pilU) [Pseudomonas aeruginosa NCGM2.S1]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382