NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	BAMOH1_RS11665	Genome accession	NZ_CP061853
Coordinates	2453537..2454607 (-)	Length	356 a.a.
NCBI ID	WP_014418378.1	Uniprot ID	A0AAP4DGV9
Organism	Bacillus amyloliquefaciens strain MOH1-5b
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2448537..2459607

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BAMOH1_RS11615 (BAMOH1_11615)	sipW	2448596..2449180 (-)	585	WP_012117977.1	signal peptidase I SipW	-
BAMOH1_RS11620 (BAMOH1_11620)	tapA	2449152..2449823 (-)	672	WP_014418371.1	amyloid fiber anchoring/assembly protein TapA	-
BAMOH1_RS11625 (BAMOH1_11625)	-	2450082..2450411 (+)	330	WP_012117979.1	DUF3889 domain-containing protein	-
BAMOH1_RS11630 (BAMOH1_11630)	-	2450452..2450631 (-)	180	WP_003153093.1	YqzE family protein	-
BAMOH1_RS11635 (BAMOH1_11635)	comGG	2450688..2451065 (-)	378	WP_017418138.1	competence type IV pilus minor pilin ComGG	Machinery gene
BAMOH1_RS11640 (BAMOH1_11640)	comGF	2451066..2451461 (-)	396	WP_025649850.1	competence type IV pilus minor pilin ComGF	-
BAMOH1_RS11645 (BAMOH1_11645)	comGE	2451475..2451789 (-)	315	WP_017418140.1	competence type IV pilus minor pilin ComGE	Machinery gene
BAMOH1_RS11650 (BAMOH1_11650)	comGD	2451773..2452210 (-)	438	WP_095061019.1	competence type IV pilus minor pilin ComGD	Machinery gene
BAMOH1_RS11655 (BAMOH1_11655)	comGC	2452200..2452466 (-)	267	WP_050496152.1	competence type IV pilus major pilin ComGC	Machinery gene
BAMOH1_RS11660 (BAMOH1_11660)	comGB	2452513..2453550 (-)	1038	WP_014418377.1	competence type IV pilus assembly protein ComGB	Machinery gene
BAMOH1_RS11665 (BAMOH1_11665)	comGA	2453537..2454607 (-)	1071	WP_014418378.1	competence type IV pilus ATPase ComGA	Machinery gene
BAMOH1_RS11670 (BAMOH1_11670)	-	2454800..2455750 (-)	951	WP_014418379.1	magnesium transporter CorA family protein	-
BAMOH1_RS11675 (BAMOH1_11675)	-	2455896..2457197 (+)	1302	WP_021494315.1	hemolysin family protein	-
BAMOH1_RS11680 (BAMOH1_11680)	-	2457235..2458065 (-)	831	WP_021494316.1	STAS domain-containing protein	-
BAMOH1_RS11690 (BAMOH1_11690)	-	2458272..2458652 (-)	381	WP_003153079.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
BAMOH1_RS11695 (BAMOH1_11695)	-	2458889..2459134 (+)	246	WP_003153078.1	DUF2626 domain-containing protein	-

Sequence

Protein

Download Length: 356 a.a. Molecular weight: 40529.81 Da Isoelectric Point: 9.3485

                                >NTDB_id=485294 BAMOH1_RS11665 WP_014418378.1 2453537..2454607(-) (comGA) [Bacillus amyloliquefaciens strain MOH1-5b]

MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS

LPLPTETVHLRMSTLPTMNDESLVIRLLPKQQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY

AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH

TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE

HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide

Download Length: 1071 bp

                                >NTDB_id=485294 BAMOH1_RS11665 WP_014418378.1 2453537..2454607(-) (comGA) [Bacillus amyloliquefaciens strain MOH1-5b]

GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC

GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG

TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT

CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT

TTTGCCTAAGCAGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCATTATTGTCCTTTCTGA

AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT

GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGATCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA

GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGACCCCGATATGATCATCTTAG

GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCCGCCATGACGGGGCACCTCGTTCTTTCAAGCCTGCAT

ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC

CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA

GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG

CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCTCTCGGCTATGTCACGACCGACAATTA

CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Bacillus subtilis subsp. subtilis str. 168	82.303	100	0.823
pilB	Glaesserella parasuis strain SC1401	38.793	97.753	0.379
pilF	Thermus thermophilus HB27	37.892	98.596	0.374

Detailed information NTG0485294