Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   H2514_RS10775 Genome accession   NZ_CP061847
Coordinates   2435359..2436417 (-) Length   352 a.a.
NCBI ID   WP_036194276.1    Uniprot ID   A0A0A0EQW5
Organism   Lysobacter sp. CW239     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2430359..2441417
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2514_RS10755 (H2514_10755) - 2431399..2433231 (-) 1833 WP_232220004.1 type IV pilus secretin PilQ family protein -
  H2514_RS10760 (H2514_10760) - 2433383..2433952 (-) 570 WP_084105628.1 pilus assembly protein PilP -
  H2514_RS10765 (H2514_10765) pilO 2433954..2434619 (-) 666 WP_036194272.1 type 4a pilus biogenesis protein PilO -
  H2514_RS10770 (H2514_10770) - 2434616..2435359 (-) 744 WP_036194274.1 PilN domain-containing protein -
  H2514_RS10775 (H2514_10775) pilM 2435359..2436417 (-) 1059 WP_036194276.1 pilus assembly protein PilM Machinery gene
  H2514_RS10780 (H2514_10780) - 2436678..2439182 (+) 2505 WP_036194279.1 penicillin-binding protein 1A -
  H2514_RS10785 (H2514_10785) - 2439303..2439995 (+) 693 WP_224743870.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37683.24 Da        Isoelectric Point: 4.5444

>NTDB_id=485086 H2514_RS10775 WP_036194276.1 2435359..2436417(-) (pilM) [Lysobacter sp. CW239]
MGVIKKSQPALVGVDISSTAVKLLQLSRAGNRYRVEHYAVEPLPPNAVVEKNIVEPEAVGEAIKRAMARSGTRAKHAAAA
VSGSAVITKVIPMPAELEGDELEAQVELEAVNYVPYPIEEVNLDFEVLGEMPGSPESVQVLLAASRSENVEVRASALELG
GLIPRVMDVEAFAIENAFGLLADQFTVPRDGLVALIDSGATMTTLNVLRNGRSVYSREQVFGGKQLTDEVMRRYSLSYEE
AGLAKRQGGLPDTYEAEVLEPFKEALVQQVSRLLQFFYAGSEFNRVDQIVLAGGCAAIAGIAAMVEEQLGVPTMIANPLA
HMTLGPRVQAHALAQDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=485086 H2514_RS10775 WP_036194276.1 2435359..2436417(-) (pilM) [Lysobacter sp. CW239]
TTGGGCGTCATCAAGAAGAGCCAGCCGGCACTCGTCGGTGTGGATATCAGTTCGACTGCGGTCAAGCTTTTGCAGCTTTC
ACGGGCGGGCAACCGCTATCGCGTGGAGCATTACGCGGTTGAGCCGCTGCCGCCCAACGCGGTGGTGGAGAAGAACATCG
TCGAGCCGGAAGCCGTGGGCGAGGCGATCAAGCGGGCGATGGCACGTTCGGGAACCCGCGCAAAACACGCCGCCGCGGCG
GTGTCCGGATCGGCGGTGATCACCAAGGTGATCCCGATGCCGGCCGAGCTGGAAGGCGACGAACTGGAAGCGCAGGTCGA
GCTGGAAGCGGTCAACTACGTGCCGTATCCGATCGAGGAAGTGAACCTCGACTTCGAAGTGCTCGGCGAGATGCCCGGCA
GTCCGGAATCGGTGCAGGTGCTGCTTGCCGCCTCGCGCTCGGAAAACGTCGAGGTCCGCGCCTCCGCGCTGGAACTTGGC
GGGCTGATCCCTAGGGTGATGGATGTGGAAGCGTTCGCGATCGAGAACGCGTTCGGCCTGCTGGCCGACCAGTTCACGGT
CCCGCGCGATGGCCTGGTGGCGCTGATCGACTCCGGCGCGACGATGACGACGCTCAACGTGCTCCGCAACGGTCGCAGCG
TGTACAGCCGCGAACAGGTGTTCGGCGGCAAGCAGTTGACCGACGAGGTCATGCGCCGCTACAGCCTCAGCTACGAGGAG
GCGGGTCTGGCCAAGCGCCAGGGCGGCTTGCCCGATACCTACGAGGCCGAAGTGTTGGAGCCGTTCAAGGAAGCCCTGGT
CCAGCAGGTCAGTCGCCTGCTGCAGTTCTTCTACGCCGGCAGCGAATTCAACCGGGTCGACCAGATCGTGCTCGCCGGTG
GCTGCGCTGCGATCGCCGGCATTGCCGCAATGGTCGAGGAGCAACTCGGGGTGCCGACGATGATCGCCAACCCGCTGGCC
CACATGACCCTGGGGCCGCGCGTGCAGGCCCATGCATTGGCCCAGGACGCGCCCGCGTTGATGATCGCCTGCGGGCTGGC
CCTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A0EQW5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.724

98.864

0.511
  comM Acinetobacter nosocomialis M2

51.437

98.864

0.509
  comM Acinetobacter baylyi ADP1

50.716

99.148

0.503
  pilM Legionella pneumophila strain ERS1305867

43.626

100

0.437