Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   J9247_RS27750 Genome accession   NZ_CP072783
Coordinates   5956216..5956839 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain LICME WGH-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5951216..5961839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J9247_RS27730 (J9247_27730) aroB 5951811..5952917 (-) 1107 WP_033977028.1 3-dehydroquinate synthase -
  J9247_RS27735 (J9247_27735) aroK 5952967..5953485 (-) 519 WP_003095833.1 shikimate kinase AroK -
  J9247_RS27740 (J9247_27740) pilQ 5953497..5955641 (-) 2145 WP_121358471.1 type 4a pilus secretin PilQ Machinery gene
  J9247_RS27745 (J9247_27745) comL/pilP 5955695..5956219 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  J9247_RS27750 (J9247_27750) pilO 5956216..5956839 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  J9247_RS27755 (J9247_27755) pilN 5956836..5957432 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  J9247_RS27760 (J9247_27760) pilM 5957432..5958496 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  J9247_RS27765 (J9247_27765) - 5958681..5961152 (+) 2472 WP_033967771.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=484943 J9247_RS27750 WP_003103268.1 5956216..5956839(-) (pilO) [Pseudomonas aeruginosa strain LICME WGH-6]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=484943 J9247_RS27750 WP_003103268.1 5956216..5956839(-) (pilO) [Pseudomonas aeruginosa strain LICME WGH-6]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGAGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401