Detailed information NTG0484901

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IC779_RS18230
• Genome accession: NZ_CP061828
• Coordinates: 3791690..3792748 (-)
• Length: 352 a.a.
• NCBI ID: WP_004705187.1
• Uniprot ID: N8R2L8
• Organism: Acinetobacter seifertii strain AS42
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3786690..3797748

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC779_RS18205 (IC779_18205)	aroK	3787002..3787571 (-)	570	WP_004705195.1	shikimate kinase AroK	-
IC779_RS18210 (IC779_18210)	pilQ	3787583..3789709 (-)	2127	WP_151792203.1	type IV pilus secretin PilQ family protein	Machinery gene
IC779_RS18215 (IC779_18215)	pilP	3789771..3790298 (-)	528	WP_077168924.1	pilus assembly protein PilP	Machinery gene
IC779_RS18220 (IC779_18220)	pilO	3790309..3791049 (-)	741	WP_077168923.1	type 4a pilus biogenesis protein PilO	Machinery gene
IC779_RS18225 (IC779_18225)	pilN	3791046..3791690 (-)	645	WP_004705190.1	PilN domain-containing protein	Machinery gene
IC779_RS18230 (IC779_18230)	pilM	3791690..3792748 (-)	1059	WP_004705187.1	pilus assembly protein PilM	Machinery gene
IC779_RS18235 (IC779_18235)	ponA	3792910..3795465 (+)	2556	WP_134262504.1	penicillin-binding protein PBP1a	-
IC779_RS18240 (IC779_18240)	-	3795483..3796307 (-)	825	WP_143221083.1	putative RNA methyltransferase	-
IC779_RS18245 (IC779_18245)	-	3796310..3796642 (-)	333	WP_077168921.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38980.49 Da
• Isoelectric Point: 4.4582

>NTDB_id=484901 IC779_RS18230 WP_004705187.1 3791690..3792748(-) (pilM) [Acinetobacter seifertii strain AS42]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAINLANPQTKNAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANSNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGVLDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLDE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=484901 IC779_RS18230 WP_004705187.1 3791690..3792748(-) (pilM) [Acinetobacter seifertii strain AS42]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTTGAAAGCTATGCTTTGATGCCTTTACCTGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTAGCGGAAGCATTGGAAAGGGCGATTAACTTAGCAAATCCCCAAACTAAAAATGCTGCAATT
GCTGTTCCTACATCGACGGTTATTCATAAAACTATCGAGATGGATGCGGATATGAGCGATGATGAACGTGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTGGATGAGGTGAGCCTAGATTTTGAGGTTCTACCTGACCGACTCG
CGAATTCAAATCGTGTAAATGTACTCTTAGTCGCAACAAGAACGGAAAACGTAGAAACGCGCGTTGAGGTTCTTGAGTTG
GCAGATTTAAATCCTAAATTGGCTGATGTTGAAAGTTATGCTGTTGAGCGCGCATTTAGTGTGTTTGCTGATAGCTTACC
TATGGGTGCAAATACGATAGGGGTTTTAGATATTGGCCATACCATGACAACATTGTCTGTCATGCAAAACGGCAAAATTA
TTTATACCCGTGAGCAAGTTTTTGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGACGAA
GCAAGTCGAGCGAAAAAAGATCGTTCTTTACCGGATGACTATGAAATTGAAGTATTAGATCCGTTTCTAGATGCCGTAGT
TCAGCAAGCGGCTCGCTCTTTACAATTCTTCTTTTCTTCATCTCAGTTTAACGAAATAGACCATATTCTGCTTGCAGGCG
GAAATGCGAATATTCCAGGTCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACTATTGCCAATCCATTTTTA
CAGATGGGTTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCTTCTTTAATGGTGGCGTGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N8R2L8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	98.295	100	0.983
comM	Acinetobacter nosocomialis M2	98.011	100	0.98
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.373	100	0.426