Detailed information NTG0484868

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: J9247_RS02055
• Genome accession: NZ_CP072783
• Coordinates: 434876..436024 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain LICME WGH-6
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 429876..441024

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J9247_RS02030 (J9247_02030)	-	431027..431323 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
J9247_RS02035 (J9247_02035)	-	431320..431913 (-)	594	WP_003084544.1	YggT family protein	-
J9247_RS02040 (J9247_02040)	proC	431924..432745 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
J9247_RS02045 (J9247_02045)	-	432757..433449 (-)	693	WP_033972290.1	YggS family pyridoxal phosphate-dependent enzyme	-
J9247_RS02050 (J9247_02050)	pilT	433664..434698 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
J9247_RS02055 (J9247_02055)	pilU	434876..436024 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
J9247_RS02060 (J9247_02060)	czcD	436032..436931 (-)	900	WP_024918014.1	CDF family zinc transporter CzcD	-
J9247_RS02065 (J9247_02065)	-	437085..437489 (+)	405	WP_003084560.1	NINE protein	-
J9247_RS02070 (J9247_02070)	-	437732..439105 (+)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
J9247_RS02075 (J9247_02075)	-	439102..440286 (+)	1185	WP_033972291.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=484868 J9247_RS02055 WP_003100959.1 434876..436024(+) (pilU) [Pseudomonas aeruginosa strain LICME WGH-6]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=484868 J9247_RS02055 WP_003100959.1 434876..436024(+) (pilU) [Pseudomonas aeruginosa strain LICME WGH-6]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGTTGTCGCCGGAACAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGACTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTTTTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAAGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGATTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTCTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAAGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCACTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382