Detailed information NTG0484128

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IC766_RS14015
• Genome accession: NZ_CP061681
• Coordinates: 2996831..2997949 (+)
• Length: 372 a.a.
• NCBI ID: WP_004703285.1
• Uniprot ID: N8S8R6
• Organism: Acinetobacter seifertii strain AS9
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2991831..3002949

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC766_RS14000 (IC766_14000)	-	2993998..2994840 (-)	843	WP_019456821.1	undecaprenyl-diphosphate phosphatase	-
IC766_RS14005 (IC766_14005)	-	2994947..2995639 (-)	693	WP_077170049.1	YggS family pyridoxal phosphate-dependent enzyme	-
IC766_RS14010 (IC766_14010)	pilT	2995766..2996803 (+)	1038	WP_019456823.1	type IV pilus twitching motility protein PilT	Machinery gene
IC766_RS14015 (IC766_14015)	pilU	2996831..2997949 (+)	1119	WP_004703285.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IC766_RS14020 (IC766_14020)	fur	2998019..2998456 (-)	438	WP_004703287.1	ferric iron uptake transcriptional regulator	-
IC766_RS14025 (IC766_14025)	-	2998568..2998966 (+)	399	WP_004703289.1	outer membrane protein assembly factor BamE	-
IC766_RS14030 (IC766_14030)	-	2999022..2999345 (-)	324	WP_019456825.1	RnfH family protein	-
IC766_RS14035 (IC766_14035)	-	2999345..3000412 (-)	1068	WP_143221137.1	hypothetical protein	-
IC766_RS14040 (IC766_14040)	-	3000565..3001020 (-)	456	WP_004703295.1	bacteriohemerythrin	-
IC766_RS14045 (IC766_14045)	-	3001271..3002119 (-)	849	WP_077170048.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41966.15 Da
• Isoelectric Point: 6.6944

>NTDB_id=484128 IC766_RS14015 WP_004703285.1 2996831..2997949(+) (pilU) [Acinetobacter seifertii strain AS9]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPSVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKTGQITYKDALKHADSPNDLRLTIKLANEGPDQIAEEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=484128 IC766_RS14015 WP_004703285.1 2996831..2997949(+) (pilU) [Acinetobacter seifertii strain AS9]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGTGTTGCACCATC
GATGAAAATTAATGGTCAAATTGTTCCCATTTCAAAGAATAGCCTTTCTGGCGAAGTAATTGGACAGCTCTTACATTCGA
TTATGAGTGAGAAGCAGCGTAAAGAATTCGCGGACACTCGTGAATGTAACTTTGCCATTATGAATCGTGACAAAACGGCA
CGTTTTCGTGTGAGTGCTTTTCAGCAGCGTGATATGCCGGGCATGGTTCTACGTCGAATTGAAACTAAAATTCCTTCGAT
TGATGACTTACAGTTACCTTCTGTGCTTAAAGATTTATCAATGACCAAACGTGGCATTATTATTTTTGTAGGCGCAACAG
GTACAGGTAAATCGACTTCGTTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGTCATATTATTACTATTGAA
GACCCTATTGAGTTTATTCACGAACATGCGGGCTGTATTATTACTCAGCGTGAGGTAGGTATTGATACTGACTCGTTCGA
AATTGCTTTAAAAAATACGTTACGACAAGCGCCCGATGTCATCTTAATTGGTGAGATCCGCTCTCGTGAAGTAATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCCACAATGCACGCCAACAACGCTAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTCTATATGGACTTATCTCTTAACTTAAAGGCCATGATTGCTCA
GCAACTTATTCCAACGCCAGATGGAAATTCACGCCGTGCAGCGATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTAATGAAACGCTCACGTGAACTCGGTATGCAAACCTTTGACCAA
GCTTTATTTGACTTATATAAAACAGGTCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTGACGATTAAGCTTGCAAATGAAGGTCCAGATCAAATTGCAGAAGAAAAACAGCATTTAACTTTTGATCGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (36-270)

3D structure

• AlphaFold DB: N8S8R6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.478	98.925	0.677
pilU	Vibrio cholerae strain A1552	55.271	94.355	0.522
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.972	95.43	0.401