Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IC767_RS15335 Genome accession   NZ_CP061678
Coordinates   3181856..3182974 (+) Length   372 a.a.
NCBI ID   WP_004703285.1    Uniprot ID   N8S8R6
Organism   Acinetobacter seifertii strain AS11     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 3127178..3215601 3181856..3182974 within 0


Gene organization within MGE regions


Location: 3127178..3215601
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC767_RS15140 (IC767_15140) - 3128201..3130114 (-) 1914 WP_190977891.1 alpha/beta fold hydrolase -
  IC767_RS15145 (IC767_15145) - 3130261..3130968 (-) 708 WP_049066035.1 YoaK family protein -
  IC767_RS15150 (IC767_15150) - 3131172..3132062 (-) 891 WP_077170071.1 LysR family transcriptional regulator -
  IC767_RS15155 (IC767_15155) - 3132250..3133194 (+) 945 WP_077170070.1 dihydrodipicolinate synthase family protein -
  IC767_RS15160 (IC767_15160) - 3133368..3134753 (+) 1386 WP_077170069.1 MFS transporter -
  IC767_RS15165 (IC767_15165) - 3134831..3136177 (+) 1347 WP_151792979.1 OprD family outer membrane porin -
  IC767_RS15170 (IC767_15170) - 3136825..3137109 (+) 285 WP_151792978.1 hypothetical protein -
  IC767_RS15175 (IC767_15175) - 3137322..3137939 (+) 618 WP_014206002.1 hypothetical protein -
  IC767_RS15180 (IC767_15180) - 3138027..3138572 (+) 546 WP_190977890.1 hypothetical protein -
  IC767_RS15185 (IC767_15185) - 3138607..3139766 (+) 1160 WP_085945698.1 IS3 family transposase -
  IC767_RS15190 (IC767_15190) - 3140062..3140931 (-) 870 WP_190977889.1 hypothetical protein -
  IC767_RS15195 (IC767_15195) - 3140982..3143888 (-) 2907 WP_190977888.1 type I restriction endonuclease subunit R -
  IC767_RS15200 (IC767_15200) - 3144014..3144925 (-) 912 WP_151792537.1 DUF5655 domain-containing protein -
  IC767_RS15205 (IC767_15205) - 3144965..3146077 (-) 1113 WP_151739591.1 PD-(D/E)XK nuclease family protein -
  IC767_RS15210 (IC767_15210) - 3146188..3146646 (-) 459 WP_151739592.1 hypothetical protein -
  IC767_RS15215 (IC767_15215) - 3146718..3147881 (-) 1164 WP_190977887.1 restriction endonuclease subunit S -
  IC767_RS15220 (IC767_15220) - 3147929..3149500 (-) 1572 WP_151792539.1 type I restriction-modification system subunit M -
  IC767_RS15225 (IC767_15225) - 3149708..3150157 (+) 450 WP_151792540.1 hypothetical protein -
  IC767_RS15230 (IC767_15230) - 3150203..3150793 (-) 591 WP_151792541.1 hypothetical protein -
  IC767_RS15235 (IC767_15235) - 3151046..3151477 (-) 432 WP_223849254.1 hypothetical protein -
  IC767_RS15240 (IC767_15240) - 3151804..3158100 (-) 6297 WP_151792543.1 DEAD/DEAH box helicase -
  IC767_RS15245 (IC767_15245) - 3158102..3161470 (-) 3369 WP_151792544.1 STY4851/ECs_5259 family protein -
  IC767_RS15250 (IC767_15250) - 3162250..3162525 (+) 276 WP_151792545.1 hypothetical protein -
  IC767_RS15260 (IC767_15260) ggt 3163101..3165083 (-) 1983 WP_151792546.1 gamma-glutamyltransferase -
  IC767_RS15265 (IC767_15265) - 3165389..3166912 (-) 1524 WP_151792547.1 DHA2 family efflux MFS transporter permease subunit -
  IC767_RS15270 (IC767_15270) - 3166919..3168070 (-) 1152 WP_019456813.1 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit -
  IC767_RS15275 (IC767_15275) - 3168341..3168838 (-) 498 WP_004703260.1 thioesterase family protein -
  IC767_RS15280 (IC767_15280) hemB 3168981..3169994 (+) 1014 WP_151792548.1 porphobilinogen synthase -
  IC767_RS15285 (IC767_15285) - 3170020..3171135 (+) 1116 WP_151792549.1 NAD(P)/FAD-dependent oxidoreductase -
  IC767_RS15290 (IC767_15290) - 3171127..3172032 (-) 906 WP_151792550.1 TIGR01777 family oxidoreductase -
  IC767_RS15295 (IC767_15295) - 3172116..3172568 (-) 453 WP_049066316.1 ABZJ_00895 family protein -
  IC767_RS15300 (IC767_15300) - 3172943..3173320 (+) 378 WP_151792551.1 VOC family protein -
  IC767_RS15305 (IC767_15305) - 3173395..3173868 (-) 474 WP_215902048.1 OsmC family protein -
  IC767_RS15310 (IC767_15310) - 3174033..3175301 (+) 1269 WP_151792552.1 exonuclease SbcCD subunit D -
  IC767_RS15315 (IC767_15315) - 3175311..3178907 (+) 3597 WP_151792553.1 SbcC/MukB-like Walker B domain-containing protein -
  IC767_RS15320 (IC767_15320) - 3179023..3179865 (-) 843 WP_019456821.1 undecaprenyl-diphosphate phosphatase -
  IC767_RS15325 (IC767_15325) - 3179972..3180664 (-) 693 WP_077170049.1 YggS family pyridoxal phosphate-dependent enzyme -
  IC767_RS15330 (IC767_15330) pilT 3180791..3181828 (+) 1038 WP_019456823.1 type IV pilus twitching motility protein PilT Machinery gene
  IC767_RS15335 (IC767_15335) pilU 3181856..3182974 (+) 1119 WP_004703285.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IC767_RS15340 (IC767_15340) fur 3183044..3183481 (-) 438 WP_004703287.1 ferric iron uptake transcriptional regulator -
  IC767_RS15345 (IC767_15345) - 3183593..3183991 (+) 399 WP_004703289.1 outer membrane protein assembly factor BamE -
  IC767_RS15350 (IC767_15350) - 3184047..3184370 (-) 324 WP_019456825.1 RnfH family protein -
  IC767_RS15355 (IC767_15355) - 3184370..3185437 (-) 1068 WP_143221137.1 hypothetical protein -
  IC767_RS15360 (IC767_15360) - 3185590..3186045 (-) 456 WP_004703295.1 bacteriohemerythrin -
  IC767_RS15365 (IC767_15365) - 3186296..3187144 (-) 849 WP_077170048.1 class II glutamine amidotransferase -
  IC767_RS15370 (IC767_15370) - 3187253..3188035 (-) 783 WP_077170047.1 GNAT family N-acetyltransferase -
  IC767_RS15375 (IC767_15375) argB 3188154..3189062 (-) 909 WP_004703301.1 acetylglutamate kinase -
  IC767_RS15380 (IC767_15380) - 3189078..3190496 (-) 1419 WP_077170046.1 phosphomannomutase/phosphoglucomutase -
  IC767_RS15385 (IC767_15385) dut 3190692..3191144 (-) 453 WP_000868152.1 dUTP diphosphatase -
  IC767_RS15390 (IC767_15390) - 3191169..3193208 (-) 2040 WP_039254369.1 UvrD-helicase domain-containing protein -
  IC767_RS15395 (IC767_15395) - 3193402..3194055 (-) 654 WP_077170045.1 OmpA family protein -
  IC767_RS15400 (IC767_15400) - 3194301..3195227 (+) 927 WP_151792554.1 acyltransferase -
  IC767_RS15405 (IC767_15405) - 3195242..3196288 (+) 1047 WP_151792555.1 preprotein translocase subunit SecA -
  IC767_RS15410 (IC767_15410) minC 3196410..3197132 (+) 723 WP_190977886.1 septum site-determining protein MinC -
  IC767_RS15415 (IC767_15415) minD 3197203..3198015 (+) 813 WP_001074362.1 septum site-determining protein MinD -
  IC767_RS15420 (IC767_15420) minE 3198018..3198290 (+) 273 WP_000896934.1 cell division topological specificity factor MinE -
  IC767_RS15425 (IC767_15425) - 3198374..3198661 (+) 288 WP_004703320.1 PA4642 family protein -
  IC767_RS15430 (IC767_15430) rhtC 3198710..3199330 (-) 621 WP_004703321.1 threonine export protein RhtC -
  IC767_RS15435 (IC767_15435) - 3199481..3202513 (-) 3033 WP_151792557.1 DNA translocase FtsK -
  IC767_RS15440 (IC767_15440) trxB 3202783..3203730 (+) 948 WP_004703323.1 thioredoxin-disulfide reductase -
  IC767_RS15445 (IC767_15445) aat 3203850..3204581 (+) 732 WP_004703324.1 leucyl/phenylalanyl-tRNA--protein transferase -
  IC767_RS15450 (IC767_15450) - 3204600..3205415 (+) 816 WP_004703325.1 arginyltransferase -
  IC767_RS15455 (IC767_15455) rimI 3205454..3205909 (-) 456 WP_077170040.1 ribosomal protein S18-alanine N-acetyltransferase -
  IC767_RS15460 (IC767_15460) - 3206018..3206893 (-) 876 WP_019456837.1 metal-dependent hydrolase -
  IC767_RS15465 (IC767_15465) - 3207132..3208013 (-) 882 WP_077170039.1 metal-dependent hydrolase -
  IC767_RS15470 (IC767_15470) tuf 3208216..3209406 (-) 1191 WP_002157016.1 elongation factor Tu -
  IC767_RS15475 (IC767_15475) fusA 3209501..3211639 (-) 2139 WP_039255563.1 elongation factor G -
  IC767_RS15480 (IC767_15480) rpsG 3211817..3212287 (-) 471 WP_001138055.1 30S ribosomal protein S7 -
  IC767_RS15485 (IC767_15485) rpsL 3212447..3212821 (-) 375 WP_002050319.1 30S ribosomal protein S12 -
  IC767_RS15490 (IC767_15490) - 3213022..3214338 (-) 1317 WP_004703334.1 EcsC family protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41966.15 Da        Isoelectric Point: 6.6944

>NTDB_id=484089 IC767_RS15335 WP_004703285.1 3181856..3182974(+) (pilU) [Acinetobacter seifertii strain AS11]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPSVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKTGQITYKDALKHADSPNDLRLTIKLANEGPDQIAEEKQHLTFDRQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=484089 IC767_RS15335 WP_004703285.1 3181856..3182974(+) (pilU) [Acinetobacter seifertii strain AS11]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGTGTTGCACCATC
GATGAAAATTAATGGTCAAATTGTTCCCATTTCAAAGAATAGCCTTTCTGGCGAAGTAATTGGACAGCTCTTACATTCGA
TTATGAGTGAGAAGCAGCGTAAAGAATTCGCGGACACTCGTGAATGTAACTTTGCCATTATGAATCGTGACAAAACGGCA
CGTTTTCGTGTGAGTGCTTTTCAGCAGCGTGATATGCCGGGCATGGTTCTACGTCGAATTGAAACTAAAATTCCTTCGAT
TGATGACTTACAGTTACCTTCTGTGCTTAAAGATTTATCAATGACCAAACGTGGCATTATTATTTTTGTAGGCGCAACAG
GTACAGGTAAATCGACTTCGTTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGTCATATTATTACTATTGAA
GACCCTATTGAGTTTATTCACGAACATGCGGGCTGTATTATTACTCAGCGTGAGGTAGGTATTGATACTGACTCGTTCGA
AATTGCTTTAAAAAATACGTTACGACAAGCGCCCGATGTCATCTTAATTGGTGAGATCCGCTCTCGTGAAGTAATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCCACAATGCACGCCAACAACGCTAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTCTATATGGACTTATCTCTTAACTTAAAGGCCATGATTGCTCA
GCAACTTATTCCAACGCCAGATGGAAATTCACGCCGTGCAGCGATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTAATGAAACGCTCACGTGAACTCGGTATGCAAACCTTTGACCAA
GCTTTATTTGACTTATATAAAACAGGTCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTGACGATTAAGCTTGCAAATGAAGGTCCAGATCAAATTGCAGAAGAAAAACAGCATTTAACTTTTGATCGACAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(36-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N8S8R6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.132

100

0.763
  pilU Pseudomonas stutzeri DSM 10701

68.478

98.925

0.677
  pilU Vibrio cholerae strain A1552

55.271

94.355

0.522
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.972

95.43

0.401