Detailed information NTG0483934

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IC772_RS14395
• Genome accession: NZ_CP061664
• Coordinates: 3134659..3135777 (+)
• Length: 372 a.a.
• NCBI ID: WP_004703285.1
• Uniprot ID: N8S8R6
• Organism: Acinetobacter seifertii strain AS28
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3129659..3140777

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC772_RS14380 (IC772_14370)	-	3131826..3132668 (-)	843	WP_068933939.1	undecaprenyl-diphosphate phosphatase	-
IC772_RS14385 (IC772_14375)	-	3132775..3133467 (-)	693	WP_191015573.1	YggS family pyridoxal phosphate-dependent enzyme	-
IC772_RS14390 (IC772_14380)	pilT	3133594..3134631 (+)	1038	WP_019456823.1	type IV pilus twitching motility protein PilT	Machinery gene
IC772_RS14395 (IC772_14385)	pilU	3134659..3135777 (+)	1119	WP_004703285.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IC772_RS14400 (IC772_14390)	fur	3135847..3136284 (-)	438	WP_191015574.1	ferric iron uptake transcriptional regulator	-
IC772_RS14405 (IC772_14395)	-	3136396..3136794 (+)	399	WP_004703289.1	outer membrane protein assembly factor BamE	-
IC772_RS14410 (IC772_14400)	-	3136850..3137173 (-)	324	WP_019456825.1	RnfH family protein	-
IC772_RS14415 (IC772_14405)	-	3137173..3138240 (-)	1068	WP_039254389.1	membrane protein	-
IC772_RS14420 (IC772_14410)	-	3138393..3138848 (-)	456	WP_004703295.1	bacteriohemerythrin	-
IC772_RS14425 (IC772_14415)	-	3139099..3139947 (-)	849	WP_019456827.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41966.15 Da
• Isoelectric Point: 6.6944

>NTDB_id=483934 IC772_RS14395 WP_004703285.1 3134659..3135777(+) (pilU) [Acinetobacter seifertii strain AS28]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPSVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKTGQITYKDALKHADSPNDLRLTIKLANEGPDQIAEEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=483934 IC772_RS14395 WP_004703285.1 3134659..3135777(+) (pilU) [Acinetobacter seifertii strain AS28]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCACCATC
TATGAAAATTAATGGTCAAATTGTTCCCATTTCAAAGAATAGCCTTTCTGGCGAAGTGATTGGACAGCTCTTACATTCGA
TTATGAGTGAGAAGCAGCGTAAAGAATTTGCGGACACTCGTGAATGTAACTTTGCCATTATGAATCGTGACAAAACGGCA
CGTTTTCGTGTGAGTGCTTTTCAGCAACGTGACATGCCGGGCATGGTTCTACGTCGAATTGAAACTAAAATTCCTTCGAT
TGATGACTTACAGTTACCTTCTGTGCTTAAAGATTTATCAATGACCAAACGTGGCATTATTATTTTTGTAGGTGCAACAG
GTACAGGTAAATCGACTTCGTTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGTCATATCATTACTATTGAA
GACCCGATTGAGTTTATTCACGAACATGCGGGCTGTATTATTACTCAGCGTGAGGTCGGTATTGATACTGACTCGTTTGA
AATTGCTTTGAAAAATACTTTACGACAAGCACCCGATGTCATCTTAATTGGTGAGATCCGTTCTCGTGAAGTAATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCACCTTGTATTAGCGACCATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCATTTCTTTGAAAGTGACCGCCACAGTCAGCTCTATATGGACTTATCTCTCAACTTAAAGGCCATGATTGCTCA
GCAGCTTATTCCAACGCCAGATGGAAATTCACGCCGTGCAGCGATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTAATGAAACGCTCACGTGAACTCGGTATGCAAACCTTTGACCAA
GCTTTATTTGACTTATATAAAACAGGTCAAATCACCTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTGACGATTAAGCTTGCAAATGAAGGTCCAGATCAAATTGCAGAAGAAAAACAGCATTTGACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (36-270)

3D structure

• AlphaFold DB: N8S8R6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.478	98.925	0.677
pilU	Vibrio cholerae strain A1552	55.271	94.355	0.522
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.972	95.43	0.401