Detailed information NTG0483910

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IC773_RS18200
• Genome accession: NZ_CP061660
• Coordinates: 3787222..3788280 (-)
• Length: 352 a.a.
• NCBI ID: WP_004705187.1
• Uniprot ID: N8R2L8
• Organism: Acinetobacter seifertii strain AS31
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3782222..3793280

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC773_RS18175 (IC773_18170)	aroK	3782534..3783103 (-)	570	WP_004705195.1	shikimate kinase AroK	-
IC773_RS18180 (IC773_18175)	pilQ	3783115..3785241 (-)	2127	WP_151792203.1	type IV pilus secretin PilQ family protein	Machinery gene
IC773_RS18185 (IC773_18180)	pilP	3785303..3785830 (-)	528	WP_077168924.1	pilus assembly protein PilP	Machinery gene
IC773_RS18190 (IC773_18185)	pilO	3785841..3786581 (-)	741	WP_077168923.1	type 4a pilus biogenesis protein PilO	Machinery gene
IC773_RS18195 (IC773_18190)	pilN	3786578..3787222 (-)	645	WP_004705190.1	PilN domain-containing protein	Machinery gene
IC773_RS18200 (IC773_18195)	pilM	3787222..3788280 (-)	1059	WP_004705187.1	pilus assembly protein PilM	Machinery gene
IC773_RS18205 (IC773_18200)	ponA	3788442..3790998 (+)	2557	Protein_3575	penicillin-binding protein PBP1a	-
IC773_RS18210 (IC773_18205)	-	3791016..3791840 (-)	825	WP_143221083.1	putative RNA methyltransferase	-
IC773_RS18215 (IC773_18210)	-	3791843..3792175 (-)	333	WP_077168921.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38980.49 Da
• Isoelectric Point: 4.4582

>NTDB_id=483910 IC773_RS18200 WP_004705187.1 3787222..3788280(-) (pilM) [Acinetobacter seifertii strain AS31]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAINLANPQTKNAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANSNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGVLDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLDE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=483910 IC773_RS18200 WP_004705187.1 3787222..3788280(-) (pilM) [Acinetobacter seifertii strain AS31]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTTGAAAGCTATGCTTTGATGCCTTTACCTGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTAGCGGAAGCATTGGAAAGGGCGATTAACTTAGCAAATCCCCAAACTAAAAATGCTGCAATT
GCTGTTCCTACATCGACGGTTATTCATAAAACTATCGAGATGGATGCGGATATGAGCGATGATGAACGTGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTGGATGAGGTGAGCCTAGATTTTGAGGTTCTACCTGACCGACTCG
CGAATTCAAATCGTGTAAATGTACTCTTAGTCGCAACAAGAACGGAAAACGTAGAAACGCGCGTTGAGGTTCTTGAGTTG
GCAGATTTAAATCCTAAATTGGCTGATGTTGAAAGTTATGCTGTTGAGCGCGCATTTAGTGTGTTTGCTGATAGCTTACC
TATGGGTGCAAATACGATAGGGGTTTTAGATATTGGCCATACCATGACAACATTGTCTGTCATGCAAAACGGCAAAATTA
TTTATACCCGTGAGCAAGTTTTTGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGACGAA
GCAAGTCGAGCGAAAAAAGATCGTTCTTTACCGGATGACTATGAAATTGAAGTATTAGATCCGTTTCTAGATGCCGTAGT
TCAGCAAGCGGCTCGCTCTTTACAATTCTTCTTTTCTTCATCTCAGTTTAACGAAATAGACCATATTCTGCTTGCAGGCG
GAAATGCGAATATTCCAGGTCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACTATTGCCAATCCATTTTTA
CAGATGGGTTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCTTCTTTAATGGTGGCGTGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N8R2L8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	98.295	100	0.983
comM	Acinetobacter nosocomialis M2	98.011	100	0.98
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.373	100	0.426