Detailed information NTG0483809

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IC776_RS04420
• Genome accession: NZ_CP061646
• Coordinates: 918787..919905 (-)
• Length: 372 a.a.
• NCBI ID: WP_004703285.1
• Uniprot ID: N8S8R6
• Organism: Acinetobacter seifertii strain AS39
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 913787..924905

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC776_RS04390 (IC776_04390)	-	914616..915464 (+)	849	WP_019456827.1	class II glutamine amidotransferase	-
IC776_RS04395 (IC776_04395)	-	915715..916170 (+)	456	WP_004703295.1	bacteriohemerythrin	-
IC776_RS04400 (IC776_04400)	-	916323..917390 (+)	1068	WP_191012727.1	hypothetical protein	-
IC776_RS04405 (IC776_04405)	-	917390..917713 (+)	324	WP_019456825.1	RnfH family protein	-
IC776_RS04410 (IC776_04410)	-	917769..918167 (-)	399	WP_004703289.1	outer membrane protein assembly factor BamE	-
IC776_RS04415 (IC776_04415)	fur	918279..918716 (+)	438	WP_004703287.1	ferric iron uptake transcriptional regulator	-
IC776_RS04420 (IC776_04420)	pilU	918787..919905 (-)	1119	WP_004703285.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IC776_RS04425 (IC776_04425)	pilT	919933..920970 (-)	1038	WP_019456823.1	type IV pilus twitching motility protein PilT	Machinery gene
IC776_RS04430 (IC776_04430)	-	921097..921789 (+)	693	WP_191012728.1	YggS family pyridoxal phosphate-dependent enzyme	-
IC776_RS04435 (IC776_04435)	-	921896..922738 (+)	843	WP_019456821.1	undecaprenyl-diphosphate phosphatase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41966.15 Da
• Isoelectric Point: 6.6944

>NTDB_id=483809 IC776_RS04420 WP_004703285.1 918787..919905(-) (pilU) [Acinetobacter seifertii strain AS39]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPSVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKTGQITYKDALKHADSPNDLRLTIKLANEGPDQIAEEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=483809 IC776_RS04420 WP_004703285.1 918787..919905(-) (pilU) [Acinetobacter seifertii strain AS39]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCACCATC
GATGAAAATTAATGGTCAAATTGTTCCCATTTCAAAGAATAGCCTTTCTGGCGAAGTGATTGGACAGCTCTTACATTCGA
TTATGAGTGAGAAGCAGCGTAAAGAATTTGCGGACACTCGTGAATGTAACTTTGCCATTATGAATCGTGACAAAACGGCA
CGTTTTCGTGTGAGTGCTTTTCAGCAACGCGATATGCCAGGCATGGTTCTACGTCGAATTGAAACTAAAATTCCTTCGAT
TGATGACTTACAGTTACCCTCTGTGCTTAAAGATTTATCAATGACCAAACGCGGCATTATTATTTTTGTAGGCGCAACAG
GTACTGGTAAATCGACTTCGTTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGTCATATCATTACTATTGAA
GACCCGATTGAGTTTATTCACGAACATGCGGGCTGTATTATTACTCAGCGTGAGGTCGGTATTGATACTGACTCGTTTGA
AATTGCTTTGAAAAATACTTTACGACAAGCACCTGATGTCATCTTAATTGGTGAGATCCGTTCTCGTGAAGTAATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCCACAATGCACGCCAACAACGCTAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTCTATATGGACTTATCTCTCAACTTAAAGGCCATGATTGCTCA
GCAACTTATTCCAACGCCAGATGGAAATTCACGCCGTGCGGCGATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTAATGAAACGCTCACGTGAACTCGGTATGCAAACCTTTGACCAA
GCTTTATTTGACTTATATAAAACAGGTCAAATTACCTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTGACGATTAAGCTTGCAAATGAAGGTCCAGATCAAATTGCAGAAGAAAAACAGCATTTGACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (36-270)

3D structure

• AlphaFold DB: N8S8R6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.478	98.925	0.677
pilU	Vibrio cholerae strain A1552	55.271	94.355	0.522
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.972	95.43	0.401