Detailed information NTG0483708

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IC780_RS18245
• Genome accession: NZ_CP061629
• Coordinates: 3793205..3794263 (-)
• Length: 352 a.a.
• NCBI ID: WP_004705187.1
• Uniprot ID: N8R2L8
• Organism: Acinetobacter seifertii strain AS47
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3788205..3799263

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC780_RS18220 (IC780_18215)	aroK	3788517..3789086 (-)	570	WP_004705195.1	shikimate kinase AroK	-
IC780_RS18225 (IC780_18220)	pilQ	3789098..3791224 (-)	2127	WP_151792203.1	type IV pilus secretin PilQ family protein	Machinery gene
IC780_RS18230 (IC780_18225)	pilP	3791286..3791813 (-)	528	WP_077168924.1	pilus assembly protein PilP	Machinery gene
IC780_RS18235 (IC780_18230)	pilO	3791824..3792564 (-)	741	WP_077168923.1	type 4a pilus biogenesis protein PilO	Machinery gene
IC780_RS18240 (IC780_18235)	pilN	3792561..3793205 (-)	645	WP_004705190.1	PilN domain-containing protein	Machinery gene
IC780_RS18245 (IC780_18240)	pilM	3793205..3794263 (-)	1059	WP_004705187.1	pilus assembly protein PilM	Machinery gene
IC780_RS18250 (IC780_18245)	ponA	3794425..3796980 (+)	2556	WP_134262504.1	penicillin-binding protein PBP1a	-
IC780_RS18255 (IC780_18250)	-	3796998..3797822 (-)	825	WP_143221083.1	putative RNA methyltransferase	-
IC780_RS18260 (IC780_18255)	-	3797825..3798157 (-)	333	WP_077168921.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38980.49 Da
• Isoelectric Point: 4.4582

>NTDB_id=483708 IC780_RS18245 WP_004705187.1 3793205..3794263(-) (pilM) [Acinetobacter seifertii strain AS47]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAINLANPQTKNAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANSNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGVLDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLDE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=483708 IC780_RS18245 WP_004705187.1 3793205..3794263(-) (pilM) [Acinetobacter seifertii strain AS47]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTTGAAAGCTATGCTTTGATGCCTTTACCTGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTAGCGGAAGCATTGGAAAGGGCGATTAACTTAGCAAATCCCCAAACTAAAAATGCTGCAATT
GCTGTTCCTACATCGACGGTTATTCATAAAACTATCGAGATGGATGCGGATATGAGCGATGATGAACGTGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTGGATGAGGTGAGCCTAGATTTTGAGGTTCTACCTGACCGACTCG
CGAATTCAAATCGTGTAAATGTACTCTTAGTCGCAACAAGAACGGAAAACGTAGAAACGCGCGTTGAGGTTCTTGAGTTG
GCAGATTTAAATCCTAAATTGGCTGATGTTGAAAGTTATGCTGTTGAGCGCGCATTTAGTGTGTTTGCTGATAGCTTACC
TATGGGTGCAAATACGATAGGGGTTTTAGATATTGGCCATACCATGACAACATTGTCTGTCATGCAAAACGGCAAAATTA
TTTATACCCGTGAGCAAGTTTTTGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGACGAA
GCAAGTCGAGCGAAAAAAGATCGTTCTTTACCGGATGACTATGAAATTGAAGTATTAGATCCGTTTCTAGATGCCGTAGT
TCAGCAAGCGGCTCGCTCTTTACAATTCTTCTTTTCTTCATCTCAGTTTAACGAAATAGACCATATTCTGCTTGCAGGCG
GAAATGCGAATATTCCAGGTCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACTATTGCCAATCCATTTTTA
CAGATGGGTTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCTTCTTTAATGGTGGCGTGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N8R2L8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	98.295	100	0.983
comM	Acinetobacter nosocomialis M2	98.011	100	0.98
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.373	100	0.426