Detailed information NTG0483642

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IC781_RS04410
• Genome accession: NZ_CP061626
• Coordinates: 937587..938705 (-)
• Length: 372 a.a.
• NCBI ID: WP_004703285.1
• Uniprot ID: N8S8R6
• Organism: Acinetobacter seifertii strain AS48
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 932587..943705

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC781_RS04380 (IC781_04380)	-	933417..934265 (+)	849	WP_019456827.1	class II glutamine amidotransferase	-
IC781_RS04385 (IC781_04385)	-	934516..934971 (+)	456	WP_004703295.1	bacteriohemerythrin	-
IC781_RS04390 (IC781_04390)	-	935124..936191 (+)	1068	WP_191000609.1	hypothetical protein	-
IC781_RS04395 (IC781_04395)	-	936191..936514 (+)	324	WP_191010922.1	RnfH family protein	-
IC781_RS04400 (IC781_04400)	-	936570..936968 (-)	399	WP_004703289.1	outer membrane protein assembly factor BamE	-
IC781_RS04405 (IC781_04405)	fur	937080..937517 (+)	438	WP_004703287.1	ferric iron uptake transcriptional regulator	-
IC781_RS04410 (IC781_04410)	pilU	937587..938705 (-)	1119	WP_004703285.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IC781_RS04415 (IC781_04415)	pilT	938733..939770 (-)	1038	WP_019456823.1	type IV pilus twitching motility protein PilT	Machinery gene
IC781_RS04420 (IC781_04420)	-	939897..940589 (+)	693	WP_191010923.1	YggS family pyridoxal phosphate-dependent enzyme	-
IC781_RS04425 (IC781_04425)	-	940696..941538 (+)	843	WP_191010924.1	undecaprenyl-diphosphate phosphatase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41966.15 Da
• Isoelectric Point: 6.6944

>NTDB_id=483642 IC781_RS04410 WP_004703285.1 937587..938705(-) (pilU) [Acinetobacter seifertii strain AS48]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPSVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKTGQITYKDALKHADSPNDLRLTIKLANEGPDQIAEEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=483642 IC781_RS04410 WP_004703285.1 937587..938705(-) (pilU) [Acinetobacter seifertii strain AS48]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCACCATC
TATGAAAATTAATGGTCAAATTGTTCCCATTTCAAAGAATAGCCTTTCTGGCGAAGTGATTGGACAGCTCTTACATTCGA
TTATGAGTGAGAAGCAGCGTAAAGAATTTGCGGACACTCGTGAATGTAACTTTGCCATTATGAATCGTGACAAAACGGCA
CGTTTTCGTGTGAGTGCTTTTCAGCAACGCGATATGCCAGGCATGGTTCTACGTCGAATTGAAACTAAAATTCCTTCGAT
TGATGACTTACAGTTACCCTCTGTGCTTAAAGATTTATCAATGACCAAACGCGGCATTATTATTTTTGTAGGCGCAACAG
GTACTGGTAAATCGACTTCGTTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGTCATATCATTACTATTGAA
GACCCGATTGAGTTTATTCACGAACATGCGGGCTGTATTATTACTCAGCGTGAGGTCGGTATTGATACTGACTCGTTCGA
AATTGCTTTGAAAAATACTTTACGACAAGCACCCGATGTCATCTTAATTGGTGAGATCCGCTCTCGTGAAGTAATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTCTTAGCCACAATGCACGCCAACAACGCTAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGACTTATCACTTAATTTAAAAGCTATGATTGCACA
GCAGCTTATTCCAACGCCAGATGGAAATTCACGCCGTGCGGCGATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACCTTTGATCAG
GCTTTATTTGACTTATATAAAACAGGTCAAATTACCTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTGACGATTAAGCTTGCAAATGAAGGTCCAGATCAAATTGCAGAAGAAAAACAGCATTTGACTTTTGATCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (36-270)

3D structure

• AlphaFold DB: N8S8R6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.478	98.925	0.677
pilU	Vibrio cholerae strain A1552	55.271	94.355	0.522
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.972	95.43	0.401