Detailed information NTG0483603

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IC782_RS04340
• Genome accession: NZ_CP061620
• Coordinates: 904184..905302 (-)
• Length: 372 a.a.
• NCBI ID: WP_004703285.1
• Uniprot ID: N8S8R6
• Organism: Acinetobacter seifertii strain AS49
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 899184..910302

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC782_RS04310 (IC782_04305)	-	900014..900862 (+)	849	WP_077170048.1	class II glutamine amidotransferase	-
IC782_RS04315 (IC782_04310)	-	901113..901568 (+)	456	WP_004703295.1	bacteriohemerythrin	-
IC782_RS04320 (IC782_04315)	-	901721..902788 (+)	1068	WP_143221137.1	hypothetical protein	-
IC782_RS04325 (IC782_04320)	-	902788..903111 (+)	324	WP_019456825.1	RnfH family protein	-
IC782_RS04330 (IC782_04325)	-	903167..903565 (-)	399	WP_004703289.1	outer membrane protein assembly factor BamE	-
IC782_RS04335 (IC782_04330)	fur	903677..904114 (+)	438	WP_004703287.1	ferric iron uptake transcriptional regulator	-
IC782_RS04340 (IC782_04335)	pilU	904184..905302 (-)	1119	WP_004703285.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IC782_RS04345 (IC782_04340)	pilT	905330..906367 (-)	1038	WP_019456823.1	type IV pilus twitching motility protein PilT	Machinery gene
IC782_RS04350 (IC782_04345)	-	906494..907186 (+)	693	WP_077170049.1	YggS family pyridoxal phosphate-dependent enzyme	-
IC782_RS04355 (IC782_04350)	-	907293..908135 (+)	843	WP_019456821.1	undecaprenyl-diphosphate phosphatase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41966.15 Da
• Isoelectric Point: 6.6944

>NTDB_id=483603 IC782_RS04340 WP_004703285.1 904184..905302(-) (pilU) [Acinetobacter seifertii strain AS49]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPSVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKTGQITYKDALKHADSPNDLRLTIKLANEGPDQIAEEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=483603 IC782_RS04340 WP_004703285.1 904184..905302(-) (pilU) [Acinetobacter seifertii strain AS49]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGTGTTGCACCATC
GATGAAAATTAATGGTCAAATTGTTCCCATTTCAAAGAATAGCCTTTCTGGCGAAGTAATTGGACAGCTCTTACATTCGA
TTATGAGTGAGAAGCAGCGTAAAGAATTCGCGGACACTCGTGAATGTAACTTTGCCATTATGAATCGTGACAAAACGGCA
CGTTTTCGTGTGAGTGCTTTTCAGCAGCGTGATATGCCGGGCATGGTTCTACGTCGAATTGAAACTAAAATTCCTTCGAT
TGATGACTTACAGTTACCTTCTGTGCTTAAAGATTTATCAATGACCAAACGTGGCATTATTATTTTTGTAGGCGCAACAG
GTACAGGTAAATCGACTTCGTTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGTCATATTATTACTATTGAA
GACCCTATTGAGTTTATTCACGAACATGCGGGCTGTATTATTACTCAGCGTGAGGTAGGTATTGATACTGACTCGTTCGA
AATTGCTTTAAAAAATACGTTACGACAAGCGCCCGATGTCATCTTAATTGGTGAGATCCGCTCTCGTGAAGTAATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCCACAATGCACGCCAACAACGCTAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTCTATATGGACTTATCTCTTAACTTAAAGGCCATGATTGCTCA
GCAACTTATTCCAACGCCAGATGGAAATTCACGCCGTGCAGCGATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTAATGAAACGCTCACGTGAACTCGGTATGCAAACCTTTGACCAA
GCTTTATTTGACTTATATAAAACAGGTCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTGACGATTAAGCTTGCAAATGAAGGTCCAGATCAAATTGCAGAAGAAAAACAGCATTTAACTTTTGATCGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (36-270)

3D structure

• AlphaFold DB: N8S8R6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.478	98.925	0.677
pilU	Vibrio cholerae strain A1552	55.271	94.355	0.522
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.972	95.43	0.401