Detailed information NTG0483502

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IC787_RS18355
• Genome accession: NZ_CP061598
• Coordinates: 3810850..3811908 (-)
• Length: 352 a.a.
• NCBI ID: WP_004705187.1
• Uniprot ID: N8R2L8
• Organism: Acinetobacter seifertii strain AS58
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3805850..3816908

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC787_RS18330 (IC787_18325)	aroK	3806162..3806731 (-)	570	WP_004705195.1	shikimate kinase AroK	-
IC787_RS18335 (IC787_18330)	pilQ	3806743..3808869 (-)	2127	WP_151792203.1	type IV pilus secretin PilQ family protein	Machinery gene
IC787_RS18340 (IC787_18335)	pilP	3808931..3809458 (-)	528	WP_077168924.1	pilus assembly protein PilP	Machinery gene
IC787_RS18345 (IC787_18340)	pilO	3809469..3810209 (-)	741	WP_077168923.1	type 4a pilus biogenesis protein PilO	Machinery gene
IC787_RS18350 (IC787_18345)	pilN	3810206..3810850 (-)	645	WP_004705190.1	PilN domain-containing protein	Machinery gene
IC787_RS18355 (IC787_18350)	pilM	3810850..3811908 (-)	1059	WP_004705187.1	pilus assembly protein PilM	Machinery gene
IC787_RS18360 (IC787_18355)	ponA	3812070..3814625 (+)	2556	WP_134262504.1	penicillin-binding protein PBP1a	-
IC787_RS18365 (IC787_18360)	-	3814643..3815467 (-)	825	WP_143221083.1	putative RNA methyltransferase	-
IC787_RS18370 (IC787_18365)	-	3815470..3815802 (-)	333	WP_077168921.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38980.49 Da
• Isoelectric Point: 4.4582

>NTDB_id=483502 IC787_RS18355 WP_004705187.1 3810850..3811908(-) (pilM) [Acinetobacter seifertii strain AS58]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAINLANPQTKNAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANSNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGVLDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLDE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=483502 IC787_RS18355 WP_004705187.1 3810850..3811908(-) (pilM) [Acinetobacter seifertii strain AS58]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTTGAAAGCTATGCTTTGATGCCTTTACCTGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTAGCGGAAGCATTGGAAAGGGCGATTAACTTAGCAAATCCCCAAACTAAAAATGCTGCAATT
GCTGTTCCTACATCGACGGTTATTCATAAAACTATCGAGATGGATGCGGATATGAGCGATGATGAACGTGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTGGATGAGGTGAGCCTAGATTTTGAGGTTCTACCTGACCGACTCG
CGAATTCAAATCGTGTAAATGTACTCTTAGTCGCAACAAGAACGGAAAACGTAGAAACGCGCGTTGAGGTTCTTGAGTTG
GCAGATTTAAATCCTAAATTGGCTGATGTTGAAAGTTATGCTGTTGAGCGCGCATTTAGTGTGTTTGCTGATAGCTTACC
TATGGGTGCAAATACGATAGGGGTTTTAGATATTGGCCATACCATGACAACATTGTCTGTCATGCAAAACGGCAAAATTA
TTTATACCCGTGAGCAAGTTTTTGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGACGAA
GCAAGTCGAGCGAAAAAAGATCGTTCTTTACCGGATGACTATGAAATTGAAGTATTAGATCCGTTTCTAGATGCCGTAGT
TCAGCAAGCGGCTCGCTCTTTACAATTCTTCTTTTCTTCATCTCAGTTTAACGAAATAGACCATATTCTGCTTGCAGGCG
GAAATGCGAATATTCCAGGTCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACTATTGCCAATCCATTTTTA
CAGATGGGTTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCTTCTTTAATGGTGGCGTGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N8R2L8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	98.295	100	0.983
comM	Acinetobacter nosocomialis M2	98.011	100	0.98
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.373	100	0.426