Detailed information NTG0483417

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IC789_RS18475
• Genome accession: NZ_CP061592
• Coordinates: 3849002..3850060 (-)
• Length: 352 a.a.
• NCBI ID: WP_004705187.1
• Uniprot ID: N8R2L8
• Organism: Acinetobacter seifertii strain AS60
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3844002..3855060

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC789_RS18450 (IC789_18445)	aroK	3844314..3844883 (-)	570	WP_004705195.1	shikimate kinase AroK	-
IC789_RS18455 (IC789_18450)	pilQ	3844895..3847021 (-)	2127	WP_151792203.1	type IV pilus secretin PilQ family protein	Machinery gene
IC789_RS18460 (IC789_18455)	pilP	3847083..3847610 (-)	528	WP_077168924.1	pilus assembly protein PilP	Machinery gene
IC789_RS18465 (IC789_18460)	pilO	3847621..3848361 (-)	741	WP_077168923.1	type 4a pilus biogenesis protein PilO	Machinery gene
IC789_RS18470 (IC789_18465)	pilN	3848358..3849002 (-)	645	WP_004705190.1	PilN domain-containing protein	Machinery gene
IC789_RS18475 (IC789_18470)	pilM	3849002..3850060 (-)	1059	WP_004705187.1	pilus assembly protein PilM	Machinery gene
IC789_RS18480 (IC789_18475)	ponA	3850222..3852777 (+)	2556	WP_134262504.1	penicillin-binding protein PBP1a	-
IC789_RS18485 (IC789_18480)	-	3852795..3853619 (-)	825	WP_143221083.1	putative RNA methyltransferase	-
IC789_RS18490 (IC789_18485)	-	3853622..3853954 (-)	333	WP_077168921.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38980.49 Da
• Isoelectric Point: 4.4582

>NTDB_id=483417 IC789_RS18475 WP_004705187.1 3849002..3850060(-) (pilM) [Acinetobacter seifertii strain AS60]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAINLANPQTKNAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANSNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGVLDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLDE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=483417 IC789_RS18475 WP_004705187.1 3849002..3850060(-) (pilM) [Acinetobacter seifertii strain AS60]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTTGAAAGCTATGCTTTGATGCCTTTACCTGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTAGCGGAAGCATTGGAAAGGGCGATTAACTTAGCAAATCCCCAAACTAAAAATGCTGCAATT
GCTGTTCCTACATCGACGGTTATTCATAAAACTATCGAGATGGATGCGGATATGAGCGATGATGAACGTGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTGGATGAGGTGAGCCTAGATTTTGAGGTTCTACCTGACCGACTCG
CGAATTCAAATCGTGTAAATGTACTCTTAGTCGCAACAAGAACGGAAAACGTAGAAACGCGCGTTGAGGTTCTTGAGTTG
GCAGATTTAAATCCTAAATTGGCTGATGTTGAAAGTTATGCTGTTGAGCGCGCATTTAGTGTGTTTGCTGATAGCTTACC
TATGGGTGCAAATACGATAGGGGTTTTAGATATTGGCCATACCATGACAACATTGTCTGTCATGCAAAACGGCAAAATTA
TTTATACCCGTGAGCAAGTTTTTGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGACGAA
GCAAGTCGAGCGAAAAAAGATCGTTCTTTACCGGATGACTATGAAATTGAAGTATTAGATCCGTTTCTAGATGCCGTAGT
TCAGCAAGCGGCTCGCTCTTTACAATTCTTCTTTTCTTCATCTCAGTTTAACGAAATAGACCATATTCTGCTTGCAGGCG
GAAATGCGAATATTCCAGGTCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACTATTGCCAATCCATTTTTA
CAGATGGGTTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCTTCTTTAATGGTGGCGTGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N8R2L8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	98.295	100	0.983
comM	Acinetobacter nosocomialis M2	98.011	100	0.98
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.373	100	0.426