Detailed information NTG0483287

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IC793_RS14255
• Genome accession: NZ_CP061571
• Coordinates: 2927150..2928268 (+)
• Length: 372 a.a.
• NCBI ID: WP_191102258.1
• Uniprot ID: -
• Organism: Acinetobacter seifertii strain AS70
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2922150..2933268

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC793_RS14240 (IC793_14235)	-	2924317..2925159 (-)	843	WP_019456821.1	undecaprenyl-diphosphate phosphatase	-
IC793_RS14245 (IC793_14240)	-	2925266..2925958 (-)	693	WP_151685379.1	YggS family pyridoxal phosphate-dependent enzyme	-
IC793_RS14250 (IC793_14245)	pilT	2926085..2927122 (+)	1038	WP_019456823.1	type IV pilus twitching motility protein PilT	Machinery gene
IC793_RS14255 (IC793_14250)	pilU	2927150..2928268 (+)	1119	WP_191102258.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IC793_RS14260 (IC793_14255)	fur	2928338..2928775 (-)	438	WP_004703287.1	ferric iron uptake transcriptional regulator	-
IC793_RS14265 (IC793_14260)	-	2928887..2929285 (+)	399	WP_004703289.1	outer membrane protein assembly factor BamE	-
IC793_RS14270 (IC793_14265)	-	2929341..2929664 (-)	324	WP_019456825.1	RnfH family protein	-
IC793_RS14275 (IC793_14270)	-	2929664..2930731 (-)	1068	WP_049063376.1	membrane protein	-
IC793_RS14280 (IC793_14275)	-	2930884..2931333 (-)	450	WP_191102554.1	bacteriohemerythrin	-
IC793_RS14285 (IC793_14280)	-	2931369..2932298 (-)	930	WP_057692912.1	IS5-like element ISAba10 family transposase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41948.12 Da
• Isoelectric Point: 6.6944

>NTDB_id=483287 IC793_RS14255 WP_191102258.1 2927150..2928268(+) (pilU) [Acinetobacter seifertii strain AS70]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPSVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGIQTFDQ
ALFDLYKTGQITYKDALKHADSPNDLRLTIKLANEGPDQIAEEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=483287 IC793_RS14255 WP_191102258.1 2927150..2928268(+) (pilU) [Acinetobacter seifertii strain AS70]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGTGTTGCACCATC
TATGAAAATTAATGGTCAAATTGTTCCCATTTCAAAGAATAGCCTTTCTGGCGAAGTGATTGGACAGCTCTTACATTCGA
TTATGAGTGAGAAGCAGCGTAAAGAATTTGCGGATACTCGTGAATGTAACTTTGCCATCATGAATCGTGACAAAACGGCA
CGTTTTCGTGTGAGTGCTTTTCAGCAACGCGATATGCCAGGCATGGTTCTACGTCGAATTGAAACTAAAATTCCTTCGAT
TGATGACTTACAGTTACCCTCTGTGCTTAAAGATTTATCAATGACCAAACGCGGCATTATTATTTTTGTAGGCGCAACAG
GTACTGGTAAATCGACTTCGTTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGTCATATCATTACTATTGAA
GACCCGATTGAGTTTATTCACGAACATGCGGGCTGTATTATTACTCAGCGTGAGGTCGGTATTGATACTGACTCATTCGA
AATTGCTTTGAAAAATACTTTACGACAAGCACCCGATGTCATCTTAATTGGTGAGATCCGTTCTCGTGAAGTAATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCACCTTGTATTAGCCACAATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCATTTCTTTGAAAGTGACCGCCACAGTCAGCTCTACATGGACTTATCTCTCAACTTAAAGGCCATGATTGCTCA
GCAGCTTATTCCAACGCCAGATGGAAATTCACGCCGTGCAGCGATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTAATGAAACGCTCACGTGAACTCGGTATTCAAACCTTTGACCAA
GCTTTATTTGACTTATATAAAACAGGTCAAATTACCTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTGACGATTAAGCTTGCAAATGAAGGTCCAGATCAAATTGCAGAAGAAAAACAGCATTTGACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (36-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	74.868	100	0.761
pilU	Pseudomonas stutzeri DSM 10701	68.207	98.925	0.675
pilU	Vibrio cholerae strain A1552	54.986	94.355	0.519
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.69	95.43	0.398