Detailed information NTG0483112

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IC798_RS01525
• Genome accession: NZ_CP061551
• Coordinates: 330192..331250 (+)
• Length: 352 a.a.
• NCBI ID: WP_004705187.1
• Uniprot ID: N8R2L8
• Organism: Acinetobacter seifertii strain AS78
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 325192..336250

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC798_RS01510 (IC798_01510)	-	326299..326631 (+)	333	WP_077163639.1	hypothetical protein	-
IC798_RS01515 (IC798_01515)	-	326634..327458 (+)	825	WP_191107329.1	putative RNA methyltransferase	-
IC798_RS01520 (IC798_01520)	ponA	327475..330030 (-)	2556	WP_191107330.1	penicillin-binding protein PBP1a	-
IC798_RS01525 (IC798_01525)	pilM	330192..331250 (+)	1059	WP_004705187.1	pilus assembly protein PilM	Machinery gene
IC798_RS01530 (IC798_01530)	pilN	331250..331894 (+)	645	WP_019457875.1	PilN domain-containing protein	Machinery gene
IC798_RS01535 (IC798_01535)	pilO	331891..332631 (+)	741	WP_191107331.1	type 4a pilus biogenesis protein PilO	Machinery gene
IC798_RS01540 (IC798_01540)	pilP	332642..333169 (+)	528	WP_004705193.1	pilus assembly protein PilP	Machinery gene
IC798_RS01545 (IC798_01545)	pilQ	333231..335357 (+)	2127	WP_191107332.1	type IV pilus secretin PilQ family protein	Machinery gene
IC798_RS01550 (IC798_01550)	aroK	335369..335938 (+)	570	WP_004705195.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38980.49 Da
• Isoelectric Point: 4.4582

>NTDB_id=483112 IC798_RS01525 WP_004705187.1 330192..331250(+) (pilM) [Acinetobacter seifertii strain AS78]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAINLANPQTKNAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANSNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGVLDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLDE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=483112 IC798_RS01525 WP_004705187.1 330192..331250(+) (pilM) [Acinetobacter seifertii strain AS78]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCTGAAAACAGTGTAGTTGAAAAAAATA
TCTTGAATCCAGAAGCAGTAGCAGAAGCATTGGAAAGGGCGATTAACTTAGCAAATCCCCAAACCAAAAATGCTGCAATT
GCTGTTCCTACATCGACGGTTATTCATAAAACTATCGAGATGGATGCTGATATGAGCGATGATGAACGTGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTGGATGAGGTGAGCCTAGATTTTGAGGTTCTACCTGACCGGCTCG
CGAATTCAAATCGTGTAAATGTACTCTTAGTCGCAACAAGAACGGAAAACGTAGAAACGCGCGTTGAGGTTCTTGAGTTG
GCAGATTTAAATCCTAAATTGGCTGATGTTGAAAGTTATGCTGTTGAGCGCGCATTTAGTGTGTTTGCTGATAGCTTACC
TATGGGTGCAAATACGATAGGGGTTTTAGATATTGGCCATACCATGACAACATTGTCTGTCATGCAAAACGGCAAAATTA
TTTATACCCGTGAGCAAGTTTTTGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGACGAA
GCAAGTCGAGCGAAAAAAGATCGCTCTTTACCAGATGACTATGAAATTGAAGTATTAGATCCGTTTCTAGATGCCGTAGT
TCAGCAAGCCGCTCGCTCTTTACAATTCTTCTTTTCCTCATCTCAGTTTAACGAAATAGACCATATTCTGCTTGCAGGCG
GAAATGCGAATATTCCAGGTCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACTATTGCCAATCCATTTTTA
CAGATGGGTTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCATCTTTAATGGTGGCGTGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	98.295	100	0.983
comM	Acinetobacter nosocomialis M2	98.011	100	0.98
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.373	100	0.426