Detailed information NTG0483045

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IC800_RS13825
• Genome accession: NZ_CP061543
• Coordinates: 2948646..2949764 (+)
• Length: 372 a.a.
• NCBI ID: WP_004703285.1
• Uniprot ID: N8S8R6
• Organism: Acinetobacter seifertii strain AS80
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2943646..2954764

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC800_RS13810 (IC800_13810)	-	2945813..2946655 (-)	843	WP_191106898.1	undecaprenyl-diphosphate phosphatase	-
IC800_RS13815 (IC800_13815)	-	2946762..2947454 (-)	693	WP_191106899.1	YggS family pyridoxal phosphate-dependent enzyme	-
IC800_RS13820 (IC800_13820)	pilT	2947581..2948618 (+)	1038	WP_019456823.1	type IV pilus twitching motility protein PilT	Machinery gene
IC800_RS13825 (IC800_13825)	pilU	2948646..2949764 (+)	1119	WP_004703285.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IC800_RS13830 (IC800_13830)	fur	2949834..2950271 (-)	438	WP_004703287.1	ferric iron uptake transcriptional regulator	-
IC800_RS13835 (IC800_13835)	-	2950383..2950781 (+)	399	WP_004703289.1	outer membrane protein assembly factor BamE	-
IC800_RS13840 (IC800_13840)	-	2950837..2951160 (-)	324	WP_019456825.1	RnfH family protein	-
IC800_RS13845 (IC800_13845)	-	2951160..2952227 (-)	1068	WP_049063376.1	membrane protein	-
IC800_RS13850 (IC800_13850)	-	2952380..2952835 (-)	456	WP_004703295.1	bacteriohemerythrin	-
IC800_RS13855 (IC800_13855)	-	2953086..2953934 (-)	849	WP_019456827.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41966.15 Da
• Isoelectric Point: 6.6944

>NTDB_id=483045 IC800_RS13825 WP_004703285.1 2948646..2949764(+) (pilU) [Acinetobacter seifertii strain AS80]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPSVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKTGQITYKDALKHADSPNDLRLTIKLANEGPDQIAEEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=483045 IC800_RS13825 WP_004703285.1 2948646..2949764(+) (pilU) [Acinetobacter seifertii strain AS80]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCACCATC
TATGAAAATTAATGGTCAAATTGTTCCCATTTCAAAGAATAGCCTTTCTGGCGAAGTGATTGGACAGCTCTTACATTCGA
TTATGAGTGAGAAGCAGCGTAAAGAATTTGCGGACACTCGTGAATGTAACTTTGCCATTATGAATCGTGACAAAACGGCA
CGTTTTCGTGTGAGTGCTTTTCAGCAACGTGACATGCCAGGCATGGTTCTACGTCGAATTGAAACTAAAATTCCTTCGAT
TGATGACTTACAGTTACCTTCTGTGCTTAAAGATTTATCAATGACCAAACGTGGCATTATTATTTTTGTAGGTGCAACAG
GTACTGGTAAATCGACTTCGTTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGTCATATCATTACTATTGAA
GACCCGATTGAGTTTATTCACGAACATGCGGGCTGTATTATTACTCAGCGTGAGGTCGGTATTGATACTGACTCGTTTGA
AATTGCTTTGAAAAATACTTTACGACAAGCACCCGATGTCATCTTAATTGGTGAGATCCGTTCTCGTGAAGTAATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCACCTTGTATTAGCGACCATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCATTTCTTTGAAAGTGACCGCCACAGTCAGCTCTATATGGACTTATCTCTCAACTTAAAGGCCATGATTGCTCA
GCAGCTTATTCCAACGCCAGATGGAAATTCACGCCGTGCAGCGATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACCTTTGACCAA
GCTTTATTTGACTTATATAAAACAGGTCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTGACGATTAAGCTTGCAAATGAAGGTCCAGATCAAATTGCAGAAGAAAAACAGCATTTGACTTTTGATCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (36-270)

3D structure

• AlphaFold DB: N8S8R6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.478	98.925	0.677
pilU	Vibrio cholerae strain A1552	55.271	94.355	0.522
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.972	95.43	0.401