Detailed information NTG0048302

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSTAA_RS20510
• Genome accession: NC_017532
• Coordinates: 4420316..4421461 (-)
• Length: 381 a.a.
• NCBI ID: WP_014598003.1
• Uniprot ID: A0A4S2B9W6
• Organism: Stutzerimonas stutzeri DSM 4166
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4415316..4426461

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSTAA_RS20485 (PSTAA_4109)	-	4415393..4416409 (+)	1017	WP_014597999.1	aspartate carbamoyltransferase catalytic subunit	-
PSTAA_RS20490 (PSTAA_4110)	-	4416409..4417683 (+)	1275	WP_014598000.1	dihydroorotase	-
PSTAA_RS20495 (PSTAA_4111)	-	4417741..4418136 (-)	396	WP_011914966.1	NINE protein	-
PSTAA_RS20500 (PSTAA_4112)	-	4418272..4419486 (+)	1215	WP_014598001.1	putative DNA modification/repair radical SAM protein	-
PSTAA_RS20505 (PSTAA_4113)	-	4419486..4420319 (+)	834	WP_014598002.1	TIGR03915 family putative DNA repair protein	-
PSTAA_RS20510 (PSTAA_4114)	pilU	4420316..4421461 (-)	1146	WP_014598003.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PSTAA_RS20515 (PSTAA_4115)	pilT	4421521..4422555 (-)	1035	WP_011914970.1	type IV pilus twitching motility protein PilT	Machinery gene
PSTAA_RS20520 (PSTAA_4116)	-	4422622..4423311 (+)	690	WP_043942282.1	YggS family pyridoxal phosphate-dependent enzyme	-
PSTAA_RS20525 (PSTAA_4117)	proC	4423322..4424146 (+)	825	WP_014598005.1	pyrroline-5-carboxylate reductase	-
PSTAA_RS20530 (PSTAA_4118)	-	4424156..4424746 (+)	591	WP_014598006.1	YggT family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42575.89 Da
• Isoelectric Point: 6.9322

>NTDB_id=48302 PSTAA_RS20510 WP_014598003.1 4420316..4421461(-) (pilU) [Stutzerimonas stutzeri DSM 4166]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTPMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNHVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=48302 PSTAA_RS20510 WP_014598003.1 4420316..4421461(-) (pilU) [Stutzerimonas stutzeri DSM 4166]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTCGAAAAAGGCGGCTCCGATCTGTTCATCACGGCTGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGAGCAAGACGCCCATGTCGCCGGAGATGACCCGGGAAACCGTGCACGGCG
TGATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAACCACGAATGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGTGTCAGCGCGTTCTATCAGCGAAACCTGGCCGGCATGGTGCTGCGTCGCATCGAGACCAACATCCCGACCATCGA
AGAGCTCAAGCTGCCGGACGTCCTGAAGAAGCTGTCGATGACCAAACGTGGTCTGGTGCTGTTCGTCGGTGCCACCGGTA
CCGGCAAGTCGACATCGCTGGCCTCGATGATCGGCTACCGCAACAAGAACTCCAGCGGGCACATCATCTCCATTGAAGAC
CCGATCGAATTCATCCACCAGCACCAGAATTGCATCGTCACCCAGCGCGAAGTGGGCATCGACACCAGCTCCTTCGAGGT
GGCCCTGAAGAACACCCTGCGCCAGGCGCCTGACGTGATCCTCATCGGCGAGATCCGTACCCGCGAAACCATGGATTACG
CGGTCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGCCGGACCGGCACAACCATGTCTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTTCCGACCCCGGACGGCAAGGGTCGTCGTGCGGTGATCGAGGTGCTGATCAATACGCCGCTGGCGGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAATCGCTGATGAAGCGCTCCACCGAGCTGGGTATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTATGTGCAGGGCGAGATCACCTACGAAGATGCCCTGCTGCATGCTGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGGGTTCGGAAACCGACGGTGAGCACCTGACCAGCGTATCCCAGGGCCTGAGCCTGGAGGTCAGCGACG
AGGACCCGGGCCGGCGCTTCCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

3D structure

• AlphaFold DB: A0A4S2B9W6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	94.751	100	0.948
pilU	Acinetobacter baylyi ADP1	65.826	93.701	0.617
pilU	Vibrio cholerae strain A1552	59.143	91.864	0.543
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.658	88.976	0.388