Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   ICJ55_RS10020 Genome accession   NZ_CP061280
Coordinates   2027282..2028595 (+) Length   437 a.a.
NCBI ID   WP_188156671.1    Uniprot ID   A0A7H1C262
Organism   Mannheimia bovis strain ZY190616     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2022282..2033595
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICJ55_RS09995 (ICJ55_09995) - 2022450..2024999 (-) 2550 WP_188156666.1 penicillin-binding protein 1A -
  ICJ55_RS10000 (ICJ55_10000) - 2025125..2025826 (+) 702 WP_188156667.1 competence protein ComA -
  ICJ55_RS10005 (ICJ55_10005) - 2025802..2026323 (+) 522 WP_188156668.1 hypothetical protein -
  ICJ55_RS10010 (ICJ55_10010) - 2026325..2026858 (+) 534 WP_188156669.1 hypothetical protein -
  ICJ55_RS10015 (ICJ55_10015) - 2026860..2027273 (+) 414 WP_188156670.1 hypothetical protein -
  ICJ55_RS10020 (ICJ55_10020) comE 2027282..2028595 (+) 1314 WP_188156671.1 type IV pilus secretin PilQ Machinery gene
  ICJ55_RS10025 (ICJ55_10025) nusB 2028675..2029088 (+) 414 WP_188156672.1 transcription antitermination factor NusB -
  ICJ55_RS10030 (ICJ55_10030) thiL 2029150..2030130 (+) 981 WP_188156673.1 thiamine-phosphate kinase -
  ICJ55_RS10035 (ICJ55_10035) - 2030136..2030609 (+) 474 WP_188156674.1 phosphatidylglycerophosphatase A -
  ICJ55_RS10040 (ICJ55_10040) - 2030615..2031241 (+) 627 WP_188156675.1 LysE family transporter -
  ICJ55_RS10045 (ICJ55_10045) ruvA 2031349..2031954 (+) 606 WP_025236421.1 Holliday junction branch migration protein RuvA -
  ICJ55_RS10050 (ICJ55_10050) ruvB 2031965..2032969 (+) 1005 WP_025236422.1 Holliday junction branch migration DNA helicase RuvB -

Sequence

Protein


Download         Length: 437 a.a.        Molecular weight: 48323.27 Da        Isoelectric Point: 7.2027

>NTDB_id=481473 ICJ55_RS10020 WP_188156671.1 2027282..2028595(+) (comE) [Mannheimia bovis strain ZY190616]
MFKIFLYLLLSSSLALADTISLSVKNAPTSLLLTYLAEETGKNIVLDDDIQTKSTLRLENKSIEEIFKTLSKVNKLSLTY
DGDIVYIHKKDEKVADLNTIPFDHLQNNGQNLPLVTTPKLITKTIKLNYAKASEVIESLTKGSGTFLSENGYIHFDERSN
SLIIKDSAKSLQNIERLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGMFSRGADHYKFGGRLEGNGLNNVANNLNVN
FPVTNGASAVLQVASINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLG
LEVVPHLSTQNQILLDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLAQKGEDKVPVLGSIPFIK
RLFSQTRDKISKRELVIFVTPYILQSNEKASGTKKSK

Nucleotide


Download         Length: 1314 bp        

>NTDB_id=481473 ICJ55_RS10020 WP_188156671.1 2027282..2028595(+) (comE) [Mannheimia bovis strain ZY190616]
ATGTTTAAAATTTTTCTTTATTTATTACTTAGCTCTTCATTAGCATTGGCTGATACTATTTCTCTTTCAGTAAAAAATGC
ACCAACTTCACTTCTGCTAACTTATTTAGCTGAAGAAACAGGAAAAAATATAGTATTAGATGATGACATTCAAACTAAAT
CCACTTTAAGATTAGAAAATAAATCTATTGAAGAGATATTTAAAACACTCAGCAAAGTGAATAAGTTATCGCTCACTTAT
GATGGAGATATCGTTTATATTCATAAAAAAGACGAAAAAGTAGCTGATTTAAATACAATACCGTTTGATCATTTGCAAAA
TAATGGGCAAAATCTACCGCTTGTAACTACACCAAAATTGATAACTAAAACGATAAAGCTCAATTATGCTAAAGCCTCAG
AAGTAATTGAATCTTTAACTAAAGGCAGTGGCACGTTTTTATCTGAGAATGGTTATATCCATTTTGATGAGCGAAGTAAT
AGCTTAATTATCAAAGATAGTGCGAAATCACTGCAAAATATTGAGCGATTAGTCAAACAACTCGACCAACCTACCGAACA
AATTGCGATTGAAGCTCGCATCGTTACTATCAGTAGTGAGCATTTGCAGGAACTAGGTGTGCGTTGGGGAATGTTTTCCA
GAGGAGCAGATCACTATAAATTTGGAGGGAGACTTGAAGGCAATGGATTAAATAATGTAGCCAATAATCTCAATGTGAAT
TTCCCCGTTACGAATGGAGCTTCCGCCGTTTTACAAGTGGCTTCCATTAATAGCCGTGTGCTAGATTTGGAACTCAGTGC
ATTAGAGCAGGAAAACAGTGTGGAAATTATTGCCAGCCCTCGTCTTTTAACCACGAATAAAAAACCGGCGAGCATCAAGC
AAGGTACAGAAATTCCTTATGTGATGTACAACACCAAATCAGAAGCCACTGATGTCGAATTTAAAGAAGCGGTGTTAGGG
TTGGAAGTTGTTCCTCATCTTTCTACTCAAAATCAAATTCTGCTGGATTTGATCGTAACCCAAAACTCACCGAACTCTCA
ATCAGGTAGCAGTGGCTTAATTACTATTGATAAACAAGAACTCAACACACAAGTTTTTGCTAAGCATGGAGAAACTATTG
TATTAGGTGGAATTTTCCAGCATTTAGCCCAAAAAGGGGAAGATAAAGTGCCAGTTTTAGGCTCAATTCCCTTTATTAAA
CGTTTATTCAGCCAAACGAGAGACAAAATCAGCAAACGAGAACTTGTGATTTTCGTTACTCCCTACATTCTTCAATCAAA
CGAAAAAGCAAGCGGTACGAAAAAATCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(267-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(122-187)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H1C262

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

63.51

99.085

0.629
  comE Haemophilus influenzae Rd KW20

49.669

100

0.515
  comE Haemophilus influenzae 86-028NP

49.007

100

0.508
  pilQ Vibrio campbellii strain DS40M4

39.72

97.941

0.389
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.905

96.339

0.384
  pilQ Vibrio cholerae strain A1552

39.905

96.339

0.384