Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IAU66_RS12510 Genome accession   NZ_CP061168
Coordinates   2595786..2596856 (-) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain T-5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2590786..2601856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IAU66_RS12460 sipW 2590845..2591429 (-) 585 WP_003153100.1 signal peptidase I SipW -
  IAU66_RS12465 tapA 2591401..2592072 (-) 672 WP_024085598.1 amyloid fiber anchoring/assembly protein TapA -
  IAU66_RS12470 - 2592332..2592661 (+) 330 WP_024085599.1 DUF3889 domain-containing protein -
  IAU66_RS12475 - 2592701..2592880 (-) 180 WP_003153093.1 YqzE family protein -
  IAU66_RS12480 comGG 2592937..2593314 (-) 378 WP_003153092.1 competence type IV pilus minor pilin ComGG Machinery gene
  IAU66_RS12485 comGF 2593315..2593815 (-) 501 WP_223203779.1 competence type IV pilus minor pilin ComGF -
  IAU66_RS12490 comGE 2593724..2594038 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  IAU66_RS12495 comGD 2594022..2594459 (-) 438 WP_024085600.1 competence type IV pilus minor pilin ComGD Machinery gene
  IAU66_RS12500 comGC 2594449..2594757 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  IAU66_RS12505 comGB 2594762..2595799 (-) 1038 WP_024085602.1 competence type IV pilus assembly protein ComGB Machinery gene
  IAU66_RS12510 comGA 2595786..2596856 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  IAU66_RS12515 - 2597048..2597998 (-) 951 WP_014305415.1 magnesium transporter CorA family protein -
  IAU66_RS12520 - 2598144..2599445 (+) 1302 WP_014305416.1 hemolysin family protein -
  IAU66_RS12525 - 2599483..2600313 (-) 831 WP_003153080.1 STAS domain-containing protein -
  IAU66_RS12535 - 2600519..2600899 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  IAU66_RS12540 - 2601136..2601381 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=481080 IAU66_RS12510 WP_003153083.1 2595786..2596856(-) (comGA) [Bacillus amyloliquefaciens strain T-5]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=481080 IAU66_RS12510 WP_003153083.1 2595786..2596856(-) (comGA) [Bacillus amyloliquefaciens strain T-5]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCACACGGCCTGCTTCTGTTTACCGGCCCGACCGGCTCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAAACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACCCGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACAACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374