Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   IAU66_RS12505 Genome accession   NZ_CP061168
Coordinates   2594762..2595799 (-) Length   345 a.a.
NCBI ID   WP_024085602.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain T-5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2589762..2600799
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IAU66_RS12455 tasA 2589996..2590781 (-) 786 WP_015388008.1 biofilm matrix protein TasA -
  IAU66_RS12460 sipW 2590845..2591429 (-) 585 WP_003153100.1 signal peptidase I SipW -
  IAU66_RS12465 tapA 2591401..2592072 (-) 672 WP_024085598.1 amyloid fiber anchoring/assembly protein TapA -
  IAU66_RS12470 - 2592332..2592661 (+) 330 WP_024085599.1 DUF3889 domain-containing protein -
  IAU66_RS12475 - 2592701..2592880 (-) 180 WP_003153093.1 YqzE family protein -
  IAU66_RS12480 comGG 2592937..2593314 (-) 378 WP_003153092.1 competence type IV pilus minor pilin ComGG Machinery gene
  IAU66_RS12485 comGF 2593315..2593815 (-) 501 WP_223203779.1 competence type IV pilus minor pilin ComGF -
  IAU66_RS12490 comGE 2593724..2594038 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  IAU66_RS12495 comGD 2594022..2594459 (-) 438 WP_024085600.1 competence type IV pilus minor pilin ComGD Machinery gene
  IAU66_RS12500 comGC 2594449..2594757 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  IAU66_RS12505 comGB 2594762..2595799 (-) 1038 WP_024085602.1 competence type IV pilus assembly protein ComGB Machinery gene
  IAU66_RS12510 comGA 2595786..2596856 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  IAU66_RS12515 - 2597048..2597998 (-) 951 WP_014305415.1 magnesium transporter CorA family protein -
  IAU66_RS12520 - 2598144..2599445 (+) 1302 WP_014305416.1 hemolysin family protein -
  IAU66_RS12525 - 2599483..2600313 (-) 831 WP_003153080.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39618.91 Da        Isoelectric Point: 9.5658

>NTDB_id=481079 IAU66_RS12505 WP_024085602.1 2594762..2595799(-) (comGB) [Bacillus amyloliquefaciens strain T-5]
MKRIKRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLKLQLHKRQLAELTDGIRRLREGDAFYEVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGELLQRKLMQTNQIKKMLRYPMFLISSVCVMFYILQSLIIPQFSGIYQSMNMNTSGATAFIFAFF
RHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPYYEKDLAAAVSHGQANGLLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=481079 IAU66_RS12505 WP_024085602.1 2594762..2595799(-) (comGB) [Bacillus amyloliquefaciens strain T-5]
ATGAAGCGGATTAAACGAACCTGGCCGTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACGGAAAAAGG
ATACAGTCTGATTGAAGGGCTCAGACTGCTGAAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGACGGACGGAATCC
GCCGGCTGAGGGAAGGTGATGCGTTTTACGAAGTGCTTGAGGCACTGTCTTTTCATAAAGAAGCCGTCTCTATCTGCTAT
TTCGCAGAGAAACACGGAGAATTGCCGGGGGCGATGAAACAAAGCGGCGAATTATTACAGAGAAAGCTCATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATTTCTTCCGTCTGTGTCATGTTTTATATCCTGCAAAGTTTAA
TTATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACATCCGGTGCGACTGCTTTCATCTTTGCATTTTTT
CGGCATTTTCATGAAGCCTGCGCACTGGCGCTGTCCGCCGCTTTCTGCTTGTTTTTGTACGTTTGGTTCTTATGTAAGAA
AAAATCCCCGCAAGATAAAATGCTCATTGTTGTGAAAATACCCCTCTTGGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTTTCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGTGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
CGGGCATCCGTATTATGAAAAAGATCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCTGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAGCGTCTTGAACAAAACGCGGCGAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

65.635

93.623

0.614