Detailed information    

insolico Bioinformatically predicted

Overview

Name   stkP   Type   Regulator
Locus tag   J5X95_RS17160 Genome accession   NZ_CP072120
Coordinates   3395998..3397941 (+) Length   647 a.a.
NCBI ID   WP_061046795.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain GKT04     
Function   require for competence development; phosphorylate ComE (predicted from homology)   
Competence regulation

Genomic Context

Location: 3390998..3402941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5X95_RS17135 (J5X95_17135) def 3391372..3391854 (+) 483 WP_007409747.1 peptide deformylase -
  J5X95_RS17140 (J5X95_17140) fmt 3391859..3392812 (+) 954 WP_061046797.1 methionyl-tRNA formyltransferase -
  J5X95_RS17145 (J5X95_17145) rsmB 3392799..3394142 (+) 1344 WP_094031702.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  J5X95_RS17150 (J5X95_17150) rlmN 3394146..3395237 (+) 1092 WP_003154339.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  J5X95_RS17155 (J5X95_17155) - 3395243..3396004 (+) 762 WP_007409750.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  J5X95_RS17160 (J5X95_17160) stkP 3395998..3397941 (+) 1944 WP_061046795.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  J5X95_RS17165 (J5X95_17165) rsgA 3397956..3398846 (+) 891 WP_061046794.1 ribosome small subunit-dependent GTPase A -
  J5X95_RS17170 (J5X95_17170) rpe 3398848..3399498 (+) 651 WP_012117536.1 ribulose-phosphate 3-epimerase -
  J5X95_RS17175 (J5X95_17175) - 3399567..3400211 (+) 645 WP_061046793.1 thiamine diphosphokinase -
  J5X95_RS17180 (J5X95_17180) spoVM 3400298..3400378 (+) 81 WP_003154329.1 stage V sporulation protein SpoVM -
  J5X95_RS17185 (J5X95_17185) rpmB 3400456..3400644 (-) 189 WP_003154328.1 50S ribosomal protein L28 -
  J5X95_RS17190 (J5X95_17190) - 3400903..3401265 (+) 363 WP_003154327.1 Asp23/Gls24 family envelope stress response protein -

Sequence

Protein


Download         Length: 647 a.a.        Molecular weight: 71665.24 Da        Isoelectric Point: 4.9835

>NTDB_id=480813 J5X95_RS17160 WP_061046795.1 3395998..3397941(+) (stkP) [Bacillus amyloliquefaciens strain GKT04]
MLTGKRISGRYHILRPIGGGGMANVFLAEDIILEREVAIKILRFDFVNDIDFIRRFRREAQSASSLDHPNIVSIYDIGEE
GDIYYIVMEYVEGMTLKEYINAHGPLHPKEALSVMEQIVSAIAHAHQNHIVHRDIKPHNILIDHLGHIKVTDFGIATALT
STTITHTNSVLGSVHYLSPEQARGGLATKKSDIYALGIVLFELLTGKIPFDGESAVSIALKHLQTETPSARKWNPSIPQS
VENIILKATAKDPFHRYESAEDMEADIRTAFDAGRLNEQKFSVQDDEEMTKAIPVITDGAKAAPQAETPGETDDKEQQPA
KKKKRKWPWVLLAVCFIFIMAAVLAVTVFPSLFMPKDVSVPDVRGMKYEKAEALLEKNGLRADPDITDIEDEKIEEELMV
KTDPKAGSTVKEGSSVKLYKSIGKPKTQLIDVKGRQIGDAKKALKEKGFKHVNVKEENDDGEAGTVIDQDPSAGTDMVAS
DDEVNLTVSLGPADVTLRDLKTYSKEAASGYLEDNGLQLVEKQAHSDDVPEGQVMKQEPAAGTAVKPGSNVEVTFSLGPE
EKPAKTVKEKISIPYEPEHEGDELEVQIAIDDKDHSISDTYDSFKIKEPTEKTIELKIDQGQKGYYQVMVDHKVVSYKTI
EYPKDND

Nucleotide


Download         Length: 1944 bp        

>NTDB_id=480813 J5X95_RS17160 WP_061046795.1 3395998..3397941(+) (stkP) [Bacillus amyloliquefaciens strain GKT04]
GTGCTAACCGGCAAGCGGATCAGCGGGCGTTATCATATCCTCCGGCCCATTGGCGGCGGCGGAATGGCAAACGTTTTTTT
AGCTGAGGACATCATTCTCGAACGTGAAGTCGCAATTAAAATTCTGCGGTTTGACTTTGTGAATGACATTGATTTTATCA
GGCGTTTCAGAAGAGAAGCACAGTCGGCATCAAGCCTCGATCATCCGAATATCGTCAGTATTTACGATATCGGGGAGGAA
GGCGATATTTATTACATTGTCATGGAATATGTGGAAGGCATGACATTAAAGGAATACATAAACGCACACGGACCGCTTCA
TCCGAAAGAAGCGCTGTCCGTTATGGAGCAGATCGTCTCGGCCATCGCCCATGCCCATCAAAACCATATCGTGCACCGGG
ATATTAAACCGCACAATATATTGATAGATCATTTAGGGCATATCAAGGTGACGGATTTCGGAATCGCCACAGCGCTTACG
TCTACGACAATCACACATACGAATTCTGTGCTGGGCTCGGTTCATTATTTGTCTCCGGAGCAGGCGAGAGGCGGTCTGGC
AACGAAAAAATCAGATATTTACGCGCTCGGCATCGTGCTGTTTGAGCTTTTAACAGGTAAAATTCCTTTTGACGGAGAAT
CAGCCGTCAGCATAGCGCTAAAGCATCTGCAAACAGAAACGCCTTCCGCCAGAAAGTGGAACCCTTCCATTCCGCAGAGC
GTCGAAAACATTATTTTAAAGGCAACCGCTAAAGACCCGTTTCACCGCTATGAAAGCGCCGAGGACATGGAAGCGGACAT
CAGAACGGCTTTTGACGCCGGCAGGCTGAATGAACAAAAATTTTCCGTGCAGGATGATGAGGAAATGACAAAAGCCATTC
CCGTTATTACTGACGGCGCAAAAGCCGCGCCTCAGGCGGAAACGCCGGGGGAGACGGATGATAAGGAACAGCAGCCAGCC
AAAAAGAAAAAACGGAAATGGCCGTGGGTTTTGCTGGCCGTCTGTTTCATTTTCATTATGGCGGCTGTTCTTGCGGTCAC
CGTGTTCCCGTCTTTATTTATGCCTAAAGATGTAAGTGTCCCCGACGTACGGGGCATGAAGTACGAAAAAGCGGAAGCCC
TGCTTGAAAAGAACGGGCTACGGGCGGATCCTGATATAACGGACATTGAAGATGAAAAAATAGAAGAGGAATTAATGGTC
AAAACGGACCCAAAAGCGGGGTCTACCGTTAAAGAAGGCTCTTCTGTAAAACTGTACAAAAGCATCGGAAAACCGAAAAC
CCAGCTGATCGACGTGAAAGGACGGCAGATCGGCGACGCAAAAAAAGCGCTGAAGGAAAAAGGCTTCAAACATGTCAATG
TCAAAGAAGAAAACGATGACGGCGAGGCCGGCACCGTAATTGATCAGGACCCGTCAGCCGGCACAGATATGGTCGCAAGC
GACGATGAAGTCAATCTAACGGTCAGCCTCGGCCCGGCGGACGTTACGCTGAGGGACTTAAAGACTTACAGCAAAGAAGC
GGCTTCAGGGTATCTTGAAGATAACGGCCTGCAGCTTGTTGAAAAACAAGCCCATTCAGATGACGTGCCGGAAGGACAAG
TCATGAAACAAGAGCCGGCAGCCGGCACTGCGGTTAAGCCGGGAAGTAATGTTGAAGTGACGTTCTCGCTCGGACCTGAA
GAAAAACCGGCGAAAACGGTAAAAGAAAAAATCAGCATTCCTTACGAACCCGAACATGAAGGGGACGAGCTTGAAGTGCA
AATCGCCATCGATGATAAGGACCACAGCATCTCTGATACGTATGACTCATTTAAAATAAAAGAGCCGACTGAAAAAACAA
TTGAACTGAAGATTGATCAGGGACAGAAGGGGTATTATCAAGTGATGGTCGATCATAAAGTCGTCAGCTACAAAACGATT
GAATACCCGAAAGATAATGACTGA

Domains

Predicted by InterProScan.

PASTA domain (PASTA)

(431-489)

PASTA domain (PASTA)

(495-556)

PASTA domain (PASTA)

(358-419)

Protein kinase domain (Pkinase)

(11-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  stkP Streptococcus pneumoniae TIGR4

38.636

95.209

0.368
  stkP Streptococcus pneumoniae D39

38.474

95.209

0.366
  stkP Streptococcus pneumoniae R6

38.474

95.209

0.366
  stkP Streptococcus pneumoniae Rx1

38.312

95.209

0.365
  pknB Streptococcus mutans UA159

40.838

88.563

0.362