Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NMBH4476_RS09750 Genome accession   NC_017516
Coordinates   1871055..1873388 (+) Length   777 a.a.
NCBI ID   WP_009348000.1    Uniprot ID   Q9ZHF3
Organism   Neisseria meningitidis H44/76     
Function   pseudopilus extrusion and retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1866055..1878388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMBH4476_RS09730 (NMBH4476_1754) pilM 1868108..1869223 (+) 1116 WP_002214517.1 type IV pilus biogenesis protein PilM -
  NMBH4476_RS09735 (NMBH4476_1755) pilN 1869226..1869825 (+) 600 WP_002214520.1 PilN domain-containing protein Machinery gene
  NMBH4476_RS09740 (NMBH4476_1756) - 1869826..1870473 (+) 648 WP_002217908.1 type 4a pilus biogenesis protein PilO -
  NMBH4476_RS09745 (NMBH4476_1757) pilP 1870491..1871036 (+) 546 WP_002214522.1 pilus assembly protein PilP Machinery gene
  NMBH4476_RS09750 (NMBH4476_1758) pilQ 1871055..1873388 (+) 2334 WP_009348000.1 type IV pilus secretin PilQ Machinery gene
  NMBH4476_RS09755 (NMBH4476_1759) - 1874290..1874802 (+) 513 WP_002222978.1 shikimate kinase -
  NMBH4476_RS09760 (NMBH4476_1760) aroB 1874882..1875961 (+) 1080 WP_002221481.1 3-dehydroquinate synthase -
  NMBH4476_RS09765 (NMBH4476_1761) - 1876928..1877770 (+) 843 WP_002226675.1 class II glutamine amidotransferase -
  NMBH4476_RS09770 (NMBH4476_1762) nrdR 1877800..1878264 (+) 465 WP_002217912.1 transcriptional regulator NrdR -

Sequence

Protein


Download         Length: 777 a.a.        Molecular weight: 83238.96 Da        Isoelectric Point: 10.1317

>NTDB_id=48070 NMBH4476_RS09750 WP_009348000.1 1871055..1873388(+) (pilQ) [Neisseria meningitidis H44/76]
MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKVSFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVL
EYADPLLSKISAAQNSSRARLVLNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
SVSEPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQAAAPAKQQAAAPAKQQAAAPAKQQAAAPAKQTNIDFRK
DGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKTPVQKVTLKRLNNDTQLIITTAGNWELVN
KSAAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWD
QALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKALLQAEKDIADLGALYSQNFQLKYKNVEEFRSILRLDNADTTGNRN
TLISGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAF
GWGVNSGFGGDDKWGAETKINLPITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
FTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTILCISTKNLNTQAMVENGGTLIVG
GIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFITPRIMGTAGNSLRY

Nucleotide


Download         Length: 2334 bp        

>NTDB_id=48070 NMBH4476_RS09750 WP_009348000.1 1871055..1873388(+) (pilQ) [Neisseria meningitidis H44/76]
ATGAATACCAAACTGACAAAAATCATTTCCGGTCTCTTTGTCGCAACCGCCGCCTTTCAGACAGCATCGGCAGGAAACAT
TACAGACATCAAAGTTTCCTCCCTGCCCAACAAACAGAAAATCGTCAAAGTCAGCTTTGACAAAGAGATTGTCAACCCGA
CCGGCTTCGTAACCTCCTCACCGGCCCGCATCGCCTTGGACTTTGAACAAACCGGCATTTCCATGGATCAACAGGTACTC
GAATATGCCGATCCTCTGTTGAGCAAAATCAGTGCCGCACAAAACAGCAGCCGTGCGCGTCTGGTTCTGAATCTGAACAA
ACCGGGCCAATACAATACCGAAGTACGCGGGAACAAAGTTTGGATATTCATTAACGAATCGGACGATACCGTGTCCGCCC
CCGCACGCCCCGCCGTAAAAGCCGCGCCTGCCGCACCGGCAAAACAACAGGCTGCCGCACCGTCTACCAAGTCCGCAGTA
TCCGTATCCGAACCCTTTACCCCGGCAAAACAACAGGCTGCCGCACCGTTTACCGAGTCCGTAGTATCCGTATCCGCACC
GTTCAGCCCGGCAAAACAACAGGCGGCGGCATCAGCAAAACAACAGGCGGCAGCACCAGCAAAACAACAGGCGGCAGCAC
CAGCAAAACAACAGGCGGCAGCACCAGCAAAACAACAGGCGGCAGCACCAGCAAAACAAACCAATATCGATTTCCGCAAA
GACGGCAAAAATGCCGGCATTATCGAATTGGCTGCATTGGGCTTTGCCGGGCAGCCCGACATCAGCCAACAGCACGACCA
CATCATCGTTACGCTGAAAAACCATACCCTGCCGACCACGCTCCAACGCAGTTTGGATGTGGCAGACTTTAAAACACCGG
TTCAAAAGGTTACGCTGAAACGCCTCAATAACGACACCCAGCTGATTATCACAACAGCCGGCAACTGGGAACTCGTCAAC
AAATCCGCCGCGCCCGGATACTTTACCTTCCAAGTCCTGCCGAAAAAACAAAACCTCGAGTCAGGCGGCGTGAACAATGC
GCCCAAAACCTTCACAGGCCGGAAAATCTCCCTTGACTTCCAAGATGTCGAAATCCGCACCATCCTGCAGATTTTGGCAA
AAGAATCCGGAATGAACATTGTTGCCAGCGACTCCGTCAACGGCAAAATGACCCTCTCCCTCAAGGATGTGCCTTGGGAT
CAGGCTTTGGATTTGGTTATGCAGGCGCGCAACCTCGATATGCGCCAGCAAGGGAATATCGTCAACATCGCGCCCCGCGA
CGAGCTGCTTGCCAAAGACAAAGCCCTCTTACAGGCAGAAAAAGACATTGCCGATTTGGGTGCGCTGTATTCCCAAAACT
TCCAGTTGAAATACAAAAATGTGGAAGAATTCCGCAGCATCCTGCGTTTGGACAATGCCGACACGACCGGAAACCGCAAC
ACGCTTATCAGCGGCAGGGGCAGCGTGCTGATCGATCCCGCCACCAACACCCTGATTGTTACCGACACCCGCAGCGTCAT
CGAAAAATTCCGCAAACTGATTGACGAATTGGACGTACCCGCGCAACAAGTGATGATTGAGGCGCGTATCGTCGAAGCGG
CAGACGGCTTCTCGCGCGATTTGGGCGTTAAATTCGGCGCGACAGGCAAGAAAAAGCTGAAAAATGATACAAGCGCATTC
GGCTGGGGGGTAAACTCCGGCTTCGGCGGCGACGATAAATGGGGGGCCGAAACCAAAATCAACCTGCCGATTACCGCTGC
CGCAAACAGCATTTCGCTGGTGCGCGCGATTTCCTCCGGTGCCTTGAATTTGGAATTGTCCGCATCCGAATCGCTTTCAA
AAACCAAAACGCTTGCCAATCCGCGCGTGCTGACCCAAAACCGCAAAGAGGCCAAAATCGAATCCGGTTACGAAATTCCT
TTCACCGTAACCTCAATCGCGAACGGCGGCAGCAGCACGAACACGGAACTCAAAAAAGCCGTCTTGGGGCTGACCGTTAC
GCCGAACATCACGCCCGACGGCCAAATCATTATGACCGTCAAAATCAACAAGGACTCGCCTGCGCAATGTGCCTCCGGTA
ATCAGACGATCCTGTGTATTTCGACCAAAAACCTGAATACGCAGGCTATGGTTGAAAACGGCGGCACATTGATTGTCGGC
GGTATTTATGAAGAAGACAACGGCAATACGCTGACCAAAGTCCCCCTGTTGGGCGACATCCCCGTTATCGGCAACCTCTT
TAAAACACGCGGGAAAAAAACCGACCGCCGCGAACTGCTGATTTTCATTACCCCGAGGATTATGGGTACGGCCGGCAACA
GCCTGCGCTATTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(609-768)

AMIN domain (AMIN)

(43-116)

Secretin and TonB N terminus short domain (STN)

(379-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(452-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9ZHF3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Neisseria meningitidis MC58

98.97

100

0.99
  pilQ Neisseria meningitidis M1080

97.169

100

0.972
  pilQ Neisseria gonorrhoeae strain FA1090

90.219

100

0.902

Multiple sequence alignment