Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NMBM04240196_RS00310 Genome accession   NC_017515
Coordinates   59846..60889 (-) Length   347 a.a.
NCBI ID   WP_002218487.1    Uniprot ID   O85741
Organism   Neisseria meningitidis M04-240196     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 54846..65889
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMBM04240196_RS00300 (NMBM04240196_0053) yccS 56021..58171 (-) 2151 WP_014581282.1 YccS family putative transporter -
  NMBM04240196_RS00305 (NMBM04240196_0054) - 58457..59683 (-) 1227 WP_002218483.1 PilT/PilU family type 4a pilus ATPase -
  NMBM04240196_RS00310 (NMBM04240196_0055) pilT 59846..60889 (-) 1044 WP_002218487.1 type IV pilus twitching motility protein PilT Machinery gene
  NMBM04240196_RS00315 (NMBM04240196_0056) - 61009..61713 (+) 705 WP_002234427.1 YggS family pyridoxal phosphate-dependent enzyme -
  NMBM04240196_RS00320 (NMBM04240196_0057) - 61695..62102 (+) 408 WP_025459756.1 hypothetical protein -
  NMBM04240196_RS00325 (NMBM04240196_0058) proC 62133..62942 (+) 810 WP_228476075.1 pyrroline-5-carboxylate reductase -
  NMBM04240196_RS00330 (NMBM04240196_0059) dksA 63089..63505 (+) 417 WP_002215267.1 RNA polymerase-binding protein DksA -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38049.53 Da        Isoelectric Point: 7.0893

>NTDB_id=47987 NMBM04240196_RS00310 WP_002218487.1 59846..60889(-) (pilT) [Neisseria meningitidis M04-240196]
MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSAEEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVAR
FRVNAFNIGRGPAAVFRTIPSTVLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAAETGHLVFGTLHTTGAAKTVDRI
VDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNGRVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQS
LQSLVRQGLIAPEVARRRAQNSESMSF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=47987 NMBM04240196_RS00310 WP_002218487.1 59846..60889(-) (pilT) [Neisseria meningitidis M04-240196]
ATGCAGATTACCGACTTACTCGCCTTCGGCGCTAAAAACAAAGCATCCGACCTTCACCTGAGTTCGGGCATATCCCCTAT
GATTCGGGTTCACGGCGATATGCGGCGCATCAACCTTCCCGAAATGAGCGCGGAAGAGGTCGGTAATATGGTAACTTCGG
TGATGAACGACCACCAGCGGAAAATCTACCAGCAAAACTTGGAAGTCGACTTCTCGTTCGAACTGCCCAACGTCGCCCGA
TTCCGCGTCAACGCCTTCAACATCGGCCGCGGTCCCGCCGCCGTATTCCGCACCATTCCCAGCACCGTCTTATCGCTGGA
AGAATTGAAAGCCCCGAGCATTTTCCAAAAAATCGCAGAATCGCCGCGCGGCATGGTTTTGGTTACCGGCCCTACCGGTT
CGGGCAAATCGACCACGCTTGCCGCAATGATCAACTACATCAACGAAACCCAGCCGGCACACATCCTGACCATCGAAGAC
CCGATCGAATTCGTCCACCAAAGCAAAAAATCCCTGATTAACCAGCGCGAGCTGCACCAGCACACCCTCAGCTTCGCCAA
CGCGCTGCGTTCCGCATTGCGCGAAGACCCCGACGTTATCCTTGTCGGCGAGATGCGCGACCCAGAAACCATCGGCTTGG
CGCTGACCGCCGCCGAAACCGGACACTTGGTTTTCGGCACGCTGCACACGACCGGCGCAGCAAAAACCGTCGACCGTATC
GTGGACGTATTCCCGGCGGGAGAAAAAGAAATGGTGCGCTCTATGCTGTCCGAATCGCTGACCGCCGTCATCTCCCAAAA
CCTGCTGAAAACGCACGACGGCAACGGCCGTGTCGCCTCGCACGAAATCCTGATTGCCAACCCCGCCGTCCGCAACCTCA
TCCGCGAAAACAAAATCACGCAGATTAACTCCGTCCTGCAAACCGGGCAGGCGAGCGGTATGCAGACAATGGACCAATCG
CTGCAATCGCTGGTGCGCCAAGGGCTGATCGCACCGGAAGTCGCACGCAGACGCGCGCAAAACAGCGAAAGTATGAGTTT
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O85741

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

100

100

1
  pilT Neisseria gonorrhoeae MS11

99.135

100

0.991
  pilT Acinetobacter baumannii D1279779

68.116

99.424

0.677
  pilT Acinetobacter baumannii strain A118

68.116

99.424

0.677
  pilT Acinetobacter nosocomialis M2

68.116

99.424

0.677
  pilT Acinetobacter baylyi ADP1

67.536

99.424

0.671
  pilT Pseudomonas stutzeri DSM 10701

67.246

99.424

0.669
  pilT Pseudomonas aeruginosa PAK

66.667

99.424

0.663
  pilT Legionella pneumophila strain Lp02

64.809

98.271

0.637
  pilT Legionella pneumophila strain ERS1305867

64.809

98.271

0.637
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.545

95.101

0.614
  pilT Vibrio cholerae strain A1552

64.545

95.101

0.614
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.644

98.847

0.53
  pilU Pseudomonas stutzeri DSM 10701

40.29

99.424

0.401
  pilU Vibrio cholerae strain A1552

38.551

99.424

0.383
  pilU Acinetobacter baylyi ADP1

38.439

99.712

0.383

Multiple sequence alignment