Detailed information NTG0479768

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: J5V74_RS08885
• Genome accession: NZ_CP071947
• Coordinates: 1940614..1941762 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 2020HL-00861
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1935614..1946762

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J5V74_RS08860 (J5V74_08860)	-	1936765..1937061 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
J5V74_RS08865 (J5V74_08865)	-	1937058..1937651 (-)	594	WP_003084544.1	YggT family protein	-
J5V74_RS08870 (J5V74_08870)	proC	1937662..1938483 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
J5V74_RS08875 (J5V74_08875)	-	1938495..1939187 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
J5V74_RS08880 (J5V74_08880)	pilT	1939402..1940436 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
J5V74_RS08885 (J5V74_08885)	pilU	1940614..1941762 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
J5V74_RS08890 (J5V74_08890)	czcD	1941770..1942669 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
J5V74_RS08895 (J5V74_08895)	-	1942823..1943227 (+)	405	WP_003084560.1	NINE protein	-
J5V74_RS08900 (J5V74_08900)	-	1943470..1944843 (+)	1374	WP_015502220.1	pyridoxal-phosphate dependent enzyme	-
J5V74_RS08905 (J5V74_08905)	-	1944840..1946024 (+)	1185	WP_023086800.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=479768 J5V74_RS08885 WP_003100959.1 1940614..1941762(+) (pilU) [Pseudomonas aeruginosa strain 2020HL-00861]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=479768 J5V74_RS08885 WP_003100959.1 1940614..1941762(+) (pilU) [Pseudomonas aeruginosa strain 2020HL-00861]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382