Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NMBM01240149_RS07515 Genome accession   NC_017514
Coordinates   1458309..1459439 (-) Length   376 a.a.
NCBI ID   WP_002214012.1    Uniprot ID   X5EQV8
Organism   Neisseria meningitidis M01-240149     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1453309..1464439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMBM01240149_RS07470 (NMBM01240149_1312) - 1453759..1454181 (-) 423 WP_002219529.1 protein Gly1ORF1 -
  NMBM01240149_RS07475 (NMBM01240149_1313) - 1454432..1454752 (-) 321 WP_002214001.1 DUF2322 family protein -
  NMBM01240149_RS07480 (NMBM01240149_1314) upp 1454775..1455401 (-) 627 WP_002219530.1 uracil phosphoribosyltransferase -
  NMBM01240149_RS07490 (NMBM01240149_1315) grxD 1455507..1455818 (-) 312 WP_002214004.1 Grx4 family monothiol glutaredoxin -
  NMBM01240149_RS07495 (NMBM01240149_1316) - 1455919..1456134 (-) 216 WP_002219531.1 DUF333 domain-containing protein -
  NMBM01240149_RS07500 (NMBM01240149_1317) - 1456160..1456939 (-) 780 WP_002219532.1 TatD family hydrolase -
  NMBM01240149_RS07505 (NMBM01240149_1318) - 1456944..1457294 (-) 351 WP_002214009.1 PilZ domain-containing protein -
  NMBM01240149_RS07510 (NMBM01240149_1319) - 1457298..1458275 (-) 978 WP_002223913.1 DNA polymerase III subunit delta' -
  NMBM01240149_RS07515 (NMBM01240149_1320) pilU 1458309..1459439 (-) 1131 WP_002214012.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NMBM01240149_RS07520 (NMBM01240149_1321) - 1459607..1460308 (+) 702 WP_002219535.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  NMBM01240149_RS07525 (NMBM01240149_1322) lepA 1460451..1462244 (+) 1794 WP_002214015.1 translation elongation factor 4 -
  NMBM01240149_RS07530 (NMBM01240149_1323) lepB 1462387..1463406 (+) 1020 WP_002221207.1 signal peptidase I -
  NMBM01240149_RS14790 - 1463625..1463774 (-) 150 WP_019273745.1 hypothetical protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42215.36 Da        Isoelectric Point: 6.4333

>NTDB_id=47966 NMBM01240149_RS07515 WP_002214012.1 1458309..1459439(-) (pilU) [Neisseria meningitidis M01-240149]
MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGDISLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=47966 NMBM01240149_RS07515 WP_002214012.1 1458309..1459439(-) (pilU) [Neisseria meningitidis M01-240149]
ATGTTTACCGATGAAAATATGACCGCAAAGGAAGAACTGTTCGCATGGCTGCGCCATATGAACAAAAACAAAGGTTCCGA
CCTGTTTGTGACGACCCATTTCCCGCCCGCAATGAAGCTGGACGGCAAAATCACCCGCATCACGGACGAACCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAATTTTCATCGACCAACGAGTGCAAC
TTCGCCATCAGCCTGCCGGACACCAGCCGCTTCCGCGTCAATGCGATGATACAGCGCGGCGCGACGGCGTTGGTATTCCG
TGCGATTACCAGCAAGATTCCCAAGTTTGAAAGCCTGAACCTGCCGCCGGTCTTGAAGGATGTCGCGCTGAAAAAACGCG
GGCTGGTTATTTTTGTCGGCGGCACCGGCTCGGGCAAATCGACTTCGCTTGCCTCGCTTATCGATTACCGCAATGAAAAT
TCGTTCGGACATATCATCACCATCGAAGACCCGATCGAGTTTGTCCACGAACACAAAAACTGCATCATCACCCAGCGCGA
GGTCGGCGTGGATACGGAAAACTGGATGGCGGCGTTGAAAAACACGCTGCGTCAGGCGCCGGATGTCATCCTTATCGGCG
AAATCCGCGACCGCGAAACGATGGACTACGCCATTGCCTTTGCCGAAACGGGGCATTTGTGTATGGCGACGCTGCACGCC
AACAGCACCAATCAGGCACTCGACCGCATCATCAACTTTTTCCCCGAGGAGCGGCGCGAACAATTGCTGACGGATTTGTC
GCTCAACCTTCAGGCGTTTATTTCGCAACGCCTCGTTCCGCGAGACGGCGGCAAGGGCAGGGTGGCGGCAGTCGAGGTGC
TGCTCAATTCGCCCCTGATTTCGGAGTTGATTCACAACGGCAACATCCATGAAATCAAAGAAGTGATGAAAAAATCCACT
ACCCTGGGTATGCAGACCTTCGATCAACACCTTTACCAATTGTATGAAAAAGGCGATATTTCCCTGCAAGAAGCATTGAA
AAATGCCGATTCCGCACACGATTTGCGTTTGGCGGTACAGTTGCGCAGCCGCCGCGCGCAAAGTTCCAGCCCCGATTTGG
AACTGCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB X5EQV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.375

93.617

0.556
  pilU Acinetobacter baylyi ADP1

57.434

91.223

0.524
  pilU Vibrio cholerae strain A1552

54.366

94.415

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.343

93.883

0.407
  pilT Legionella pneumophila strain Lp02

43.827

86.17

0.378
  pilT Legionella pneumophila strain ERS1305867

43.827

86.17

0.378
  pilT Acinetobacter baylyi ADP1

42.727

87.766

0.375
  pilT Pseudomonas aeruginosa PAK

42.683

87.234

0.372
  pilT Acinetobacter nosocomialis M2

42.901

86.17

0.37
  pilT Acinetobacter baumannii D1279779

42.901

86.17

0.37
  pilT Acinetobacter baumannii strain A118

42.901

86.17

0.37
  pilT Neisseria meningitidis 8013

40.896

89.096

0.364
  pilT Pseudomonas stutzeri DSM 10701

41.768

87.234

0.364

Multiple sequence alignment