Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   H8B22_RS09625 Genome accession   NZ_CP060820
Coordinates   2094940..2096013 (+) Length   357 a.a.
NCBI ID   WP_187711217.1    Uniprot ID   -
Organism   Agrilutibacter terrestris strain II4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2089940..2101013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8B22_RS09615 (H8B22_09615) - 2091125..2091784 (-) 660 WP_187711215.1 hypothetical protein -
  H8B22_RS09620 (H8B22_09620) - 2091974..2094493 (-) 2520 WP_187711216.1 penicillin-binding protein 1A -
  H8B22_RS09625 (H8B22_09625) pilM 2094940..2096013 (+) 1074 WP_187711217.1 pilus assembly protein PilM Machinery gene
  H8B22_RS09630 (H8B22_09630) - 2096013..2096702 (+) 690 WP_187711218.1 PilN domain-containing protein -
  H8B22_RS09635 (H8B22_09635) - 2096699..2097391 (+) 693 WP_187711219.1 type 4a pilus biogenesis protein PilO -
  H8B22_RS09640 (H8B22_09640) - 2097393..2097944 (+) 552 WP_187711220.1 pilus assembly protein PilP -
  H8B22_RS09645 (H8B22_09645) - 2097960..2099888 (+) 1929 WP_407060806.1 type IV pilus secretin PilQ -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 38236.82 Da        Isoelectric Point: 4.6830

>NTDB_id=478617 H8B22_RS09625 WP_187711217.1 2094940..2096013(+) (pilM) [Agrilutibacter terrestris strain II4]
MGRKIVGIVTKGQPTLVGVDISSTAVKLLQLSKAGDRYRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIKRAVNRSGTKVK
YAAAAVAGSSVITKVIPMPADLDEDDLESQVELEAVNYVPYPIEEVNLDFEVLGPMANNPGTLQVLLAASRSENVELRSS
ALEIGGLTPKVMDVEAFAIENAFALLANQLSGPRDGLTALIDSGATMTTLNVLRNGRSLYSREQVFGGKQLTDEVMRRYG
LSYEEAGLAKRQGGLPESYQAEVLEPFKEAMVQQVSRLLQFFYAGSEFNRVDQIVLAGGNAAIPGIAQMVEEHLGVPTLV
ANPLAHMTLGPRVQAHALAQDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=478617 H8B22_RS09625 WP_187711217.1 2094940..2096013(+) (pilM) [Agrilutibacter terrestris strain II4]
ATGGGGAGAAAAATCGTGGGGATCGTCACAAAAGGCCAGCCGACCCTCGTCGGCGTGGACATCAGTTCGACCGCGGTGAA
GCTGTTGCAGCTCTCGAAGGCTGGCGACCGCTACCGCGTCGAGCACTATGCGGTCGAGCCGCTGCCGCCGAATGCCGTGG
TCGAGAAGAACATCGTCGAGGTCGAGGCGGTGGGCGAGGCGATCAAGCGCGCGGTCAATCGCTCCGGCACCAAGGTCAAG
TACGCCGCTGCTGCGGTGGCGGGCTCGTCGGTCATCACCAAGGTGATCCCGATGCCGGCCGATCTCGACGAGGACGACCT
CGAATCGCAGGTCGAACTCGAGGCCGTCAACTACGTGCCGTACCCGATCGAGGAAGTGAACCTCGATTTCGAGGTGCTCG
GCCCGATGGCCAACAACCCGGGCACGCTGCAGGTGCTGCTCGCGGCGTCCCGTTCGGAGAACGTCGAACTGCGCTCCTCG
GCGCTGGAAATCGGCGGCCTCACCCCGAAGGTCATGGACGTGGAAGCGTTCGCGATCGAGAACGCGTTCGCGCTGCTCGC
CAACCAGCTGAGTGGCCCACGCGACGGCCTGACCGCGCTGATCGACTCCGGCGCGACGATGACCACGCTCAACGTCCTGC
GCAACGGCCGCAGCCTGTACAGCCGCGAACAGGTGTTCGGCGGCAAGCAGCTCACCGACGAAGTGATGCGCCGTTACGGA
CTCAGCTACGAGGAAGCGGGTCTGGCCAAGCGCCAGGGCGGCCTGCCGGAAAGCTACCAGGCCGAAGTGCTCGAGCCCTT
CAAGGAAGCCATGGTCCAGCAGGTGAGCCGCCTGCTGCAGTTCTTCTATGCCGGCAGCGAGTTCAACCGCGTCGACCAGA
TCGTGCTCGCCGGCGGCAACGCGGCCATTCCGGGCATCGCCCAGATGGTCGAGGAGCACCTCGGCGTGCCCACGCTCGTA
GCCAACCCGCTGGCGCACATGACGCTCGGTCCCCGCGTGCAGGCGCACGCACTGGCGCAGGATGCACCTGCGCTGATGAT
CGCCTGTGGCCTCGCCCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(18-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.754

95.798

0.496
  comM Acinetobacter nosocomialis M2

51.754

95.798

0.496
  comM Acinetobacter baylyi ADP1

50.585

95.798

0.485
  pilM Legionella pneumophila strain ERS1305867

45.058

96.359

0.434