Detailed information NTG0478535

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IAQ69_RS08305
• Genome accession: NZ_CP060811
• Coordinates: 1770593..1771771 (-)
• Length: 392 a.a.
• NCBI ID: WP_200228602.1
• Uniprot ID: A0A7T8AQQ5
• Organism: Acinetobacter variabilis strain XM9F202-2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1765593..1776771

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IAQ69_RS08285 (IAQ69_08280)	-	1766006..1767034 (+)	1029	WP_200228599.1	AraC family transcriptional regulator	-
IAQ69_RS08290 (IAQ69_08285)	gcvH	1767117..1767491 (+)	375	WP_104426083.1	glycine cleavage system protein GcvH	-
IAQ69_RS08295 (IAQ69_08290)	-	1767744..1768919 (+)	1176	WP_200228601.1	MFS transporter	-
IAQ69_RS08300 (IAQ69_08295)	pilU	1768993..1770189 (-)	1197	WP_125278619.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IAQ69_RS08305 (IAQ69_08300)	pilU	1770593..1771771 (-)	1179	WP_200228602.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IAQ69_RS08310 (IAQ69_08305)	-	1772027..1773076 (-)	1050	WP_159122908.1	3-deoxy-7-phosphoheptulonate synthase	-
IAQ69_RS08315 (IAQ69_08310)	-	1773615..1774139 (-)	525	WP_159122907.1	hypothetical protein	-
IAQ69_RS08320 (IAQ69_08315)	-	1774370..1775482 (-)	1113	WP_180053222.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-

Sequence

Protein

• Length: 392 a.a.
• Molecular weight: 44404.80 Da
• Isoelectric Point: 6.2880

>NTDB_id=478535 IAQ69_RS08305 WP_200228602.1 1770593..1771771(-) (pilU) [Acinetobacter variabilis strain XM9F202-2]
MYTTELLDEARKFMHHMLTKVVEYGSSDLFITADFPPSIKQQGLMKPFGQQKLTADKTRLFAYALMNERQIQEFESVMEC
NFSISIPGVSRFRVNVFQQQHNVGMVIRIITTDIPSFEQLGLPDSLQQVIMQKRGLVLVVGATGVGKSTTLAAMIDYRNT
HTEGHIITIEDPVEYVYQHKNSMITQREIGVDCVSWHNALINTMRQAPDVIVIGEIRDTDSMEHAIAFAESGHLCLATLH
SNNADQALDRIINFFPEERRNQLLLDLSANIKAVISQRLIRTEDGKGRRAAVEILQNTPLVSDLIRKGEFHELKTIMARS
RELGMQTFDQALFDLYNQGIISYEEALRNADSSNELRLQIKLKSSRINPVLQAENAQISLLEQSRSRALSPD

Nucleotide

• Length: 1179 bp

>NTDB_id=478535 IAQ69_RS08305 WP_200228602.1 1770593..1771771(-) (pilU) [Acinetobacter variabilis strain XM9F202-2]
ATGTATACAACTGAATTACTCGATGAAGCACGTAAATTTATGCACCATATGCTGACCAAAGTCGTGGAATATGGTAGTTC
CGACCTTTTTATCACTGCTGATTTCCCGCCCAGTATCAAACAGCAAGGGTTAATGAAACCGTTTGGCCAGCAGAAACTCA
CAGCCGATAAGACCCGTTTGTTTGCTTATGCCTTGATGAATGAGCGACAGATTCAGGAATTTGAATCGGTGATGGAATGC
AATTTTAGTATTAGCATCCCGGGCGTGTCGCGTTTCCGGGTTAATGTCTTTCAGCAACAGCACAATGTAGGCATGGTGAT
TCGAATCATTACGACAGATATTCCCAGCTTTGAGCAACTAGGCTTACCGGATTCATTGCAACAGGTCATCATGCAGAAAC
GTGGTTTGGTGCTGGTAGTTGGTGCAACGGGTGTCGGGAAATCGACTACATTGGCAGCCATGATTGATTACCGTAATACT
CATACTGAAGGGCATATTATTACGATCGAAGACCCGGTGGAATATGTCTATCAGCATAAAAACTCGATGATCACCCAGCG
TGAAATTGGTGTAGATTGCGTTTCCTGGCACAATGCACTGATTAATACCATGCGGCAGGCACCTGACGTCATCGTGATTG
GCGAAATCAGGGATACAGACAGTATGGAGCATGCAATTGCCTTTGCTGAATCTGGCCATTTATGTCTTGCAACACTCCAT
TCTAATAATGCCGATCAGGCACTGGACCGGATTATTAACTTCTTTCCAGAAGAACGCCGTAATCAGTTACTGCTAGATTT
ATCAGCTAATATCAAAGCCGTGATTTCTCAGCGGCTAATTCGCACTGAAGATGGTAAAGGTCGTCGGGCTGCGGTAGAAA
TTCTGCAAAATACTCCTTTAGTTTCTGACCTGATTCGTAAAGGTGAATTCCATGAACTTAAAACGATTATGGCTCGTTCG
CGGGAACTGGGCATGCAAACCTTTGATCAGGCATTATTTGATTTATATAACCAAGGCATTATTTCTTATGAAGAAGCCTT
GCGTAATGCAGACTCCAGCAATGAATTACGTTTGCAAATCAAGCTGAAGAGCAGTCGTATCAATCCTGTTTTACAGGCAG
AAAATGCCCAGATTTCACTTCTGGAACAGTCCAGATCGCGTGCACTTTCTCCTGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (52-282)

3D structure

• AlphaFold DB: A0A7T8AQQ5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	60.057	88.776	0.533
pilU	Acinetobacter baylyi ADP1	54.648	90.561	0.495
pilU	Vibrio cholerae strain A1552	51.831	90.561	0.469
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.353	86.735	0.367