Detailed information NTG0476640

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: HUG14_RS02885
• Genome accession: NZ_CP060580
• Coordinates: 580675..581121 (-)
• Length: 148 a.a.
• NCBI ID: WP_058203710.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain K_LL005
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 575675..586121

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUG14_RS02850 (HUG14_02850)	-	575792..576169 (-)	378	WP_033900715.1	pyridoxamine 5'-phosphate oxidase family protein	-
HUG14_RS02855 (HUG14_02855)	-	576374..577240 (+)	867	WP_033900713.1	RluA family pseudouridine synthase	-
HUG14_RS02860 (HUG14_02860)	-	577279..578088 (-)	810	WP_033900712.1	metal ABC transporter permease	-
HUG14_RS02865 (HUG14_02865)	-	578081..578818 (-)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
HUG14_RS02870 (HUG14_02870)	-	578995..579837 (-)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
HUG14_RS02875 (HUG14_02875)	-	579834..580271 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
HUG14_RS02880 (HUG14_02880)	comGG	580352..580636 (-)	285	WP_017865155.1	competence type IV pilus minor pilin ComGG	Machinery gene
HUG14_RS02885 (HUG14_02885)	comGF	580675..581121 (-)	447	WP_058203710.1	competence type IV pilus minor pilin ComGF	Machinery gene
HUG14_RS02890 (HUG14_02890)	comGE	581084..581380 (-)	297	WP_033900709.1	competence type IV pilus minor pilin ComGE	Machinery gene
HUG14_RS02895 (HUG14_02895)	comGD	581352..581750 (-)	399	WP_233610962.1	competence type IV pilus minor pilin ComGD	Machinery gene
HUG14_RS02900 (HUG14_02900)	comGC	581743..582126 (-)	384	WP_080735951.1	competence type IV pilus major pilin ComGC	Machinery gene
HUG14_RS02905 (HUG14_02905)	comGB	582140..583213 (-)	1074	WP_080735950.1	competence type IV pilus assembly protein ComGB	Machinery gene
HUG14_RS02910 (HUG14_02910)	comGA	583107..584045 (-)	939	WP_033900707.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17181.02 Da
• Isoelectric Point: 9.0871

>NTDB_id=476640 HUG14_RS02885 WP_058203710.1 580675..581121(-) (comGF) [Lactococcus lactis strain K_LL005]
MERKLCNLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQDFL
YVTKDKKLRFGFMRDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=476640 HUG14_RS02885 WP_058203710.1 580675..581121(-) (comGF) [Lactococcus lactis strain K_LL005]
ATGGAAAGGAAATTATGCAACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAGCAGGATTTTCTG
TATGTAACTAAAGACAAAAAACTAAGGTTTGGTTTTATGAGAGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAGAGAACATTTATTTATCAATTTCCGGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	75.54	93.919	0.709
comYF	Streptococcus mutans UA140	47.518	95.27	0.453
comYF	Streptococcus mutans UA159	46.809	95.27	0.446
comGF/cglF	Streptococcus mitis SK321	41.606	92.568	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385
comGF/cglF	Streptococcus pneumoniae Rx1	39.007	95.27	0.372
comGF/cglF	Streptococcus pneumoniae D39	39.007	95.27	0.372
comGF/cglF	Streptococcus pneumoniae R6	39.007	95.27	0.372
comGF/cglF	Streptococcus pneumoniae TIGR4	39.007	95.27	0.372