Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilB   Type   Machinery gene
Locus tag   H8Z70_RS05255 Genome accession   NZ_CP060460
Coordinates   1228308..1228427 (+) Length   39 a.a.
NCBI ID   WP_005915819.1    Uniprot ID   A0A7S6YV99
Organism   Xanthomonas citri pv. citri strain DAR84832     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1228656..1250293 1228308..1228427 flank 229


Gene organization within MGE regions


Location: 1228308..1250293
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8Z70_RS05255 (H8Z70_05255) pilB 1228308..1228427 (+) 120 WP_005915819.1 hypothetical protein Machinery gene
  H8Z70_RS05260 (H8Z70_05260) - 1228559..1228726 (+) 168 WP_015463523.1 hypothetical protein -
  H8Z70_RS05265 (H8Z70_05265) pilR 1228949..1230343 (-) 1395 WP_005930970.1 sigma-54 dependent transcriptional regulator Regulator
  H8Z70_RS05270 (H8Z70_05270) - 1230668..1232281 (-) 1614 WP_003488599.1 HAMP domain-containing sensor histidine kinase -
  H8Z70_RS05275 (H8Z70_05275) sucC 1232513..1233682 (+) 1170 WP_005915812.1 ADP-forming succinate--CoA ligase subunit beta -
  H8Z70_RS05280 (H8Z70_05280) sucD 1233707..1234582 (+) 876 WP_005921370.1 succinate--CoA ligase subunit alpha -
  H8Z70_RS23430 - 1234702..1234851 (-) 150 WP_157380096.1 hypothetical protein -
  H8Z70_RS05290 (H8Z70_05290) - 1235139..1236254 (-) 1116 WP_011052122.1 IS1595 family transposase -
  H8Z70_RS05295 (H8Z70_05295) - 1236482..1236766 (-) 285 WP_016849322.1 hypothetical protein -
  H8Z70_RS05305 (H8Z70_05305) xopAI 1238097..1238987 (-) 891 WP_076605129.1 type III secretion system effector XopAI -
  H8Z70_RS05310 (H8Z70_05310) - 1239121..1240223 (-) 1103 Protein_1039 DNA-binding protein -
  H8Z70_RS05315 (H8Z70_05315) xopE 1240554..1241630 (-) 1077 WP_041471283.1 XopE/AvrPphe family type III secretion system effector -
  H8Z70_RS05320 (H8Z70_05320) - 1241764..1242847 (-) 1084 Protein_1041 DNA-binding protein -
  H8Z70_RS05325 (H8Z70_05325) - 1243040..1244313 (+) 1274 Protein_1042 site-specific integrase -
  H8Z70_RS05330 (H8Z70_05330) - 1244322..1247312 (+) 2991 WP_015472512.1 Tn3 family transposase -
  H8Z70_RS05335 (H8Z70_05335) - 1247445..1248722 (+) 1278 WP_005919631.1 lytic murein transglycosylase -
  H8Z70_RS05340 (H8Z70_05340) avrXacE2 1248807..1249877 (+) 1071 WP_011052114.1 type III secretion system effector avirulence protein AvrXacE2 -

Sequence


Protein


Download         Length: 39 a.a.        Molecular weight: 4178.84 Da        Isoelectric Point: 9.0113

>NTDB_id=476246 H8Z70_RS05255 WP_005915819.1 1228308..1228427(+) (pilB) [Xanthomonas citri pv. citri strain DAR84832]
MQIAEAAQAIGIRDLRQSALMKAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 120 bp        

>NTDB_id=476246 H8Z70_RS05255 WP_005915819.1 1228308..1228427(+) (pilB) [Xanthomonas citri pv. citri strain DAR84832]
ATGCAGATCGCCGAGGCCGCACAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCGTTGATGAAGGCTGCGCACGGGGT
GACCAGCCTGGCGGAGATCAATCGTGTGACGAAGGACTAA

Domains



No domain identified.



Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A7S6YV99

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

51.282

100

0.513

  pilB Acinetobacter baumannii D1279779

48.718

100

0.487

  pilB Vibrio cholerae strain A1552

45.714

89.744

0.41

  pilB Legionella pneumophila strain ERS1305867

38.462

100

0.385